Vascularization in tissue engineering

Rouwkema, Jeroen; Rivron, Nicolas; Blitterswijk, Clemens A. van

doi:10.1016/j.tibtech.2008.04.009

Cited by 1,067 publications

(811 citation statements)

References 62 publications

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“…However, such constructs have often failed to produce the desired results because of issues such as the poor biocompatibility and immunogenicity of the biomaterials used, and cell necrosis at the bulk of the scaffold related to deficient oxygen and nutrient diffusion. [3][4][5][6][7] Oxygen and nutrient supply is a critical issue when creating thick-engineered tissues such as the bone. 1,2 The consequence of this problem is that successful production of tissue-engineered products is virtually limited to thin tissues such as the skin.…”

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confidence: 99%

“…1,2 The consequence of this problem is that successful production of tissue-engineered products is virtually limited to thin tissues such as the skin. 7 This scenario illustrates how vascularization is a major hurdle of bone tissue engineering. An approach that has been increasingly studied to overcome this issue is the in vitro prevascularization of the constructs.…”

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confidence: 99%

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Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets

Pirraco

Iwata

Yoshida

et al. 2014

Laboratory Investigation

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Addressing the problem of vascularization is of vital importance when engineering three-dimensional (3D) tissues. Endothelial cells are increasingly used in tissue-engineered constructs to obtain prevascularization and to enhance in vivo neovascularization. Rat bone marrow stromal cells were cultured in thermoresponsive dishes under osteogenic conditions with human umbilical vein endothelial cells (HUVECs) to obtain homotypic or heterotypic cell sheets (CSs). Cells were retrieved as sheets from the dishes after incubation at 20 1C. Monoculture osteogenic CSs were stacked on top of homotypic or heterotypic CSs, and subcutaneously implanted in the dorsal flap of nude mice for 7 days. The implants showed mineralized tissue formation under both conditions. Transplanted osteogenic cells were found at the new tissue site, demonstrating CS bone-inductive effect. Perfused vessels, positive for human CD31, confirmed the contribution of HUVECs for the neovascularization of coculture CS constructs. Furthermore, calcium quantification and expression of osteocalcin and osterix genes were higher for the CS constructs, with HUVECs demonstrating the more robust osteogenic potential of these constructs. This work demonstrates the potential of using endothelial cells, combined with osteogenic CSs, to increase the in vivo vascularization of CS-based 3D constructs for bone tissue engineering purposes.

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confidence: 99%

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confidence: 99%

Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets

Pirraco

Iwata

Yoshida

et al. 2014

Laboratory Investigation

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“…It has been estimated that a distance of less than 200 μM from the supplying capillary is the critical distance for diffusion of oxygen and nutrients to any new tissue introduced into the body. Because of this, the survival of any three-dimensional tissue graft relies on rapid development of new blood vessels to supply not only the center but also the margins of the graft [73].…”

Section: Tissue Engineering Approaches To Design Novel Materials To Bmentioning

confidence: 99%

Synthetic Materials Used in the Surgical Treatment of Pelvic Organ Prolapse: Problems of Currently Used Material and Designing the Ideal Material

Mangır¹,

Chapple²,

MacNeil³

2018

Pelvic Floor Disorders

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Synthetic materials have long been used to provide structural support when surgically repairing pelvic organ prolapse (POP). The most widely used synthetic material is a mesh made of polypropylene (PPL). The use of mesh is intended to improve cure rates and prevent recurrences after POP surgery -however as more mesh materials have been implanted, it has become apparent that serious complications can occur in up to 30% of women, particularly when the mesh is implanted transvaginally. Over the years many different mesh kits have been marketed and used in the treatment of POP however polypropylene mesh was never designed or tested for use in pelvic floor. Instead it was approved for clinical use based on its biocompatibility and success in abdominal hernia repairs. It is now known that PPL meshes are neither compliant with the mechanical forces in the pelvic floor nor do they integrate well into paravaginal tissues. Better materials developed specifically for use in pelvic floor are urgently needed. The aim of this chapter is to define the requirements of an ideal mesh in terms of its material properties and to summarize the ongoing research on developing the next generation pelvic floor repair materials.

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“…The classification of above mentioned porosity was based on literatures and dimensions: macroporosity (≥ 100μm, the least required size for cell survivability [29,30]), microporosity (1 ~ 100μm, allowing in-growth of human micro-vessels or capillaries [31]) and nanoporosity (< 1μm, significantly promoting osteoinductivity through enhancing osteogenic differentiation [13,14]). …”

Section: Particle Size Of Porogenmentioning

confidence: 99%

Fabrication and characterization of 3D complex hydroxyapatite scaffolds with hierarchical porosity of different features for optimal bioactive performance

Chen

Zhang

Yang

et al. 2017

Ceramics International

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Vascularization in tissue engineering

Cited by 1,067 publications

References 62 publications

Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets

Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets

Synthetic Materials Used in the Surgical Treatment of Pelvic Organ Prolapse: Problems of Currently Used Material and Designing the Ideal Material

Fabrication and characterization of 3D complex hydroxyapatite scaffolds with hierarchical porosity of different features for optimal bioactive performance

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