Vascularization of Hepatocellular Carcinoma Tissue Depends on Its Differentiation Degree

Щеголев, А. И.; Дубова, Е. А.; Туманова, У.Н.

doi:10.1007/s10517-012-1749-x

Cited by 6 publications

(5 citation statements)

References 12 publications

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“…The above changes confirm the data above about the change of immunophenotype of endothelial cells during hepatocarcinogenesis. According to our previous studies, the degree of HCC vascularization depends on the degree of its histological differentiation: with a decrease in the degree of HCC differentiation, there is a progressive decline in the number of blood vessels [9].…”

Section: Discussionmentioning

confidence: 90%

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Computed Tomography Assessment of the Vascularization Degree of Hepatocellular Carcinoma in the Noncirrhotic Liver

Туманова¹

2015

AJCEM

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An important CT-characteristic at a diagnostic of liver tumors is the determination of its vascularity. To study the characteristics of blood tissue of hepatocellular carcinoma (HCC) with different degrees of differentiation by CT. The work is based on the clinical and morphological comparisons performed in 55 patients (34 men and 21 women aged 15-83 years) with hepatocellular carcinoma (HCC) without accompanying liver cirrhosis. In the preoperative stage all patients was carried spiral computed tomography (CT) with bolus contrast enhancement (on the four phases of the study). Was evaluated parameters of CT density and degree of nodes HCC vascularization. Morphologic study of histological preparations of operation material from 16 patients was diagnosed high-differentiated, 33-moderately differentiated, and 6-poorly differentiated HCC. At the immunohistochemical preparations with reaction to CD34 was determined by the number and the total cross-sectional area of vessels in sight of HCC tissue. The maximum values of CT density in the arterial phase were observed at the nodes moderately differentiated HCC. In the venous phase the greatest values of density as well as the venous increase was registered in highdifferentiated HCC. The most vascularised (hypervascular) tumor is high-differentiated HCC, and the least vascularizedpoorly differentiated HCC by CT. At morphometric study of the maximum values of the number and the total cross-sectional area of the blood vessels are installed in high-differentiated HCC tissues, the minimum-in the tissue of low-grade HCC. CT with bolus contrast enhancement allows studying the characteristics of blood supply of HCC varying degrees of differentiation. Determine the true measure of total CT-vascularization of the tumor tissue must be done with consideration of the increase concentration difference coefficient (ICDC).

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Section: Discussionmentioning

confidence: 90%

“…Therefore, the identification of HCC development, progression, and correction processes associated with angiogenesis is an important task [7,8]. We have previously demonstrated that the morphological characteristics of HCC vascularization depend on the degree o histological differentiation [9].…”

Section: Introductionmentioning

confidence: 99%

Computed Tomography Assessment of the Vascularization Degree of Hepatocellular Carcinoma in the Noncirrhotic Liver

Туманова¹

2015

AJCEM

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“…The angiogenic activity of HCC correlates with the risk of metastasis and prognostic significance [27]. Previous research reported that anti-angiogenesis might be highly efficient for the treatment of HCC by inhibiting angiogenesis in tumor tissues [28e30].…”

Section: Discussionmentioning

confidence: 99%

Regulation of tumorigenesis and metastasis of hepatocellular carcinoma tumor endothelial cells by microRNA-3178 and underlying mechanism

Shen

et al. 2015

Biochemical and Biophysical Research Communications

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“…The growth and metastasis of malignant tumors relies on angiogenesis ( 1 , 2 ). When the tumor volume exceeds 2–3 mm 3 , due to the ensuing ischemia and hypoxia, new capillaries are generated to maintain tumor growth ( 3 , 4 ).…”

Section: Introductionmentioning

confidence: 99%

“…An abundance of blood vessels may provide enough nutrients to the tumor tissue and remove various degradation products, and tumor cells can also undergo hematogenous metastasis after they enter the circulation via the blood vessels; otherwise, the tumor may undergo degenerative necrosis ( 5 ). The proliferation, invasion and metastasis of malignant tumors are closely related to tumor-induced angiogenesis ( 1 , 2 ). Neovascularization provides sufficient oxygen and nutrients for the proliferation, infiltration and metastasis of tumor cells ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

Fisetin inhibits vascular endothelial growth factor‑induced angiogenesis in retinoblastoma cells

2020

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Fisetin is a small phytochemical molecule with antitumor activity. Angiogenesis is a basic process that occurs during tumor growth and metastasis. The vascular endothelial growth factor (VEGF)/VEGF receptor (VEGFR) pathway is a key regulator of angiogenesis. The aim of the present study was to evaluate whether fisetin affects angiogenesis through the VEGFR pathway. In the present study, Y79 cells were treated with 100 ng/ml VEGF in the presence of fisetin at concentrations of 0, 25, 50 and 100 µM. A Cell Counting Kit-8 assay was used to detect proliferation and the Transwell and Matrigel assays were used to assess cell migration and invasion, respectively. Reverse transcription-quantitative polymerase chain reaction analysis was applied to measure the expression level of VEGFR mRNA and western blot analysis was used to measure the protein expression of VEGFR. An immunofluorescence assay was used to detect the expression of VEGFR. Angiogenesis in vitro was assessed by a tube formation assay. The results demonstrated that fisetin significantly inhibited the proliferation of Y79 cells in a time- and dose-dependent manner. Fisetin also inhibited the migration and invasion of Y79 cells in a dose-dependent manner. Furthermore, fisetin inhibited the expression of VEGFR in Y79 cells in a dose-dependent manner and tumor angiogenesis in vitro . Thus, fisetin was found to inhibit angiogenesis via inhibition of the VEGF/VEGFR signaling pathway, and could be used as a candidate drug to inhibit angiogenesis in retinoblastoma.

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Vascularization of Hepatocellular Carcinoma Tissue Depends on Its Differentiation Degree

Cited by 6 publications

References 12 publications

Computed Tomography Assessment of the Vascularization Degree of Hepatocellular Carcinoma in the Noncirrhotic Liver

Computed Tomography Assessment of the Vascularization Degree of Hepatocellular Carcinoma in the Noncirrhotic Liver

Regulation of tumorigenesis and metastasis of hepatocellular carcinoma tumor endothelial cells by microRNA-3178 and underlying mechanism

Fisetin inhibits vascular endothelial growth factor‑induced angiogenesis in retinoblastoma cells

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