VASOPRESSIN IN BLOOD: EFFECT OF HEMORRHAGE¹

Abstract

A method has been developed for the estimation of vasopressin in blood. By means of a few simple chemical and chromatographic procedures vasopressin contained in large quantities of blood may be extracted and obtained in small volumes of solution suitable for either bioassay determinations or further purification. These methods coupled with a bioassay procedure have been employed for the studj' of the time course of changes in the concentration of pressor material in the blood after hemorrhage. Evidence is presented that this pressor material is identical with vasopressin. From the results of these studies, an approximate rate and total discharge of vasopressin into blood of dogs subjected to hemorrhage was calculated.

The regulation of antidiuretic hormone release in man

“…The eluent is lyophilized and taken up in 1.0 ml of a solution containing 0.85% NaCl and 0.03% acetic acid. Weinstein et al (10) have demonstrated, and we have confirmed in isolated experiments, that the material contained in the eluent has the biological and chemical properties of vasopressin. It is soluble in 10% TCA, insoluble in ether, stable to the action of pepsin, and rapidly inactivated by trypsin and by 0.01 M thioglycollic acid.…”

Central Mechanism of Vasopressin‐induced Changes in Antidiuretic Hormone Release

“…In another set of experiments, in order to elicit the full antidiuretic effect of higher doses of intracerebroventricular (i.c.v. ) vasopressin, the urine flow rate was kept at a higher level by the administration of dextrose-saline (Bhargava, Kulshrestha & Srivastava, 1972;Bhargava, Kulshrestha, Santhakumari & Srivastava, 1973 Weinstein, Berne & Sachs (1960) as modified by Yoshida, Motohashi, Ibayashi & Okinaka (1963). This method of extraction was found to give a recovery of 80% ADH in controls and results were modified accordingly.…”

Stimulation-dependent uptake of an extracellular marker to subcellular fractions of isolated neurohypophysial tissue

“…Although fresh rat neurohypophyses can easily be obtained, a major problem in work with the gland from these animals is the minute amount of tissue (< 1 mg/neural lobe) and difficulties in separating pars intermedia from the neural lobe. Therefore, biochemical analyses and subfractionation studies have been carried out mainly with bovine neurohypophyses (150 mg/neural lobe), with the drawback that the hemorrhage during slaughtering stimulates neurohypophyses to release hormone 19 . Despite this fact, we have found an increased uptake of HRP into previously characterized subcellular fractions from bovine neurohypophyses 16 which have the advantage that they can be freed completely of tissue from the intermediate lobe.…”