Vasovasostomy in Rabbits using Fibrin Adhesive Prepared from a Single Human Source

Abstract

We were interested in determining whether fibrin glue derived from a single human source could be used effectively to produce a sutureless vasovasostomy in the rabbit model. Fifteen rabbits were divided into two groups (7 control, 8 experimental) and underwent vasal transection and reanastomosis by conventional microsurgical suture techniques (control group) and sutureless anastomosis using single source fibrin glue (experimental group). The patency and tensile strength of the anastomoses were compared at four weeks and the anastomotic sites underwent histologic examination. This study demonstrated that a vasovasal anastomosis in the rabbit could be achieved using human single source fibrin glue with patency equivalent to a standard sutured anastomosis. The tensile strength of the glued anastomosis at four weeks was greater than the tensile strength of the sutured anastomosis.

Delayed Vasovasostomy: Experimental Study Using Fibrin Glue

“…Recent ex perimental studies involving immediate vasectomy rever sal protocols have been performed in rabbits [8,9] and rats [10,11], These studies have shown that fibrin glue offered several advantages compared with the use of microsutures.…”

Vasal Reanastomosis Using Fibrin Glue Combined with Sutures: Which Combination of Sutures in a Delayed Protocol?

“…Recent experimental attempts to improve the technical demand and the time commitment of suture vasovasostomy include the use of fibrin glue combined either with stent [4, 5, 6] or sutures [1, 7, 8]. The protocol used in most of these studies was an immediate vasectomy reversal.…”

“…Only two experimental studies to date have investigated a delayed vasovasostomy protocol [1, 2]. The results are summarized in table 4 [1, 2, 4, 5, 6, 7, 8, 9]. …”

Vasovasostomy

“…Other studies have employed bio-glues (fibrin glue) and resorbable stents in animal experiments [60,[63][64][65][66][67]. Most investigators have concluded from these studies that bio-glues are as successful for anastomosis as conventional sutures [60,[64][65][66][67], except for the study by Borau Durá n and Ponce de Leon Roca [63].…”

“…Most investigators have concluded from these studies that bio-glues are as successful for anastomosis as conventional sutures [60,[64][65][66][67], except for the study by Borau Durá n and Ponce de Leon Roca [63].…”