Vector Transmission of Grapevine Leafroll-Associated Viruses

Herrbach, Étienne; Alliaume, Antoine; Prator, Cecilia A.; Daane, Kent M.; Cooper, Monica L.; Almeida, Rodrigo P. P.

doi:10.1007/978-3-319-57706-7_24

Cited by 39 publications

(41 citation statements)

References 69 publications

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“…Taxonomically GLRaVs belong to the Closteroviridae family which is split in 3 genera: Ampelovirus (classifying GLRaV-1, -3, -4 and -13) Closterovirus (classifying GLRaV-2) and Velarivirus (classifying GLRaV-7) [3]. Leafroll viruses are phloem-restricted single-stranded RNA viruses, and ampeloviruses are transmitted by mealybugs or soft scale insects in a semi-persistent manner [23]. GLRaV-3 is recognized as the main etiological agent contributing to 1 3 GLD due to its high prevalence in many grapevine-growing areas in Europe, the Americas, Middle East, Northern and Southern parts of Africa, Asia and Oceania [38].…”

Section: Introductionmentioning

confidence: 99%

A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.

et al. 2018

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We have characterized the virome of a grapevine Pinot Noir accession (P70) that displayed, over the year, very stable and strong leafroll symptoms. For this, we have used two extraction methods (dsRNA and total RNA) coupled with the high throughput sequencing (HTS) Illumina technique. While a great disparity in viral sequences were observed, both approaches gave similar results, revealing a very complex infection status. Five virus and viroid isolates [Grapevine leafroll-associated viruse-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine rupestris stem pitting-associated virus (GRSPaV), Hop stunt viroid (HSVd) and Grapevine yellow speckle viroid 1 (GYSVd1)] were detected in P70 with a grand total of eleven variants being identified and de novo assembled. A comparison between both extraction methods regarding their power to detect viruses and the ease of genome assembly is also provided.

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Section: Introductionmentioning

confidence: 99%

A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.

et al. 2018

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Section: Introductionmentioning

confidence: 99%

“…Within the scale insects (Coccoidea), many species of mealybugs (Pseudococcidae) and soft scales (Coccidae) feeding on grapevine were shown to transmit GLD ampeloviruses in different grapevine‐growing regions in the world (Herrbach et al, ; Herrbach, Le Maguet, & Hommay, ) and represent at the local scale a non‐negligible way of virus dispersal (Almeida et al, ). Moreover, these piercing‐sucking insects are known to transmit three grapevine‐infecting viruses associated to “rugose wood complex”: Grapevine virus A (GVA), GVB and GVE (Herrbach et al, ), assigned to the genus Vitivirus . It has been demonstrated that, although scale species are unable to fly (except adult males which do not feed and therefore are not vectors), some mealybug species are able to rapidly spread the disease within plots and from infected plots to new plantations (Cabaleiro et al, ; Golino, Weber, Sim, & Rowhani, ; Le Maguet et al, , ).…”

Section: Introductionmentioning

confidence: 99%

Gone with the wind: Aerial dispersal of Parthenolecanium corni crawlers in a newly planted grapevine plot

Hommay¹,

Wiss²,

Maguet

et al. 2019

Annals of Applied Biology

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Soft scales nymphs are suspected to propagate grapevine leafroll viruses in the vineyard, while moving from vine to vine, but results of long distance dispersion, especially those due to wind, are poorly known. Thus, a net of sticky cylindrical traps was placed in a newly planted grapevine plot to evaluate aerial dispersal of Parthenolecanium corni (Hemiptera Coccidae) nymphs. The plot was surrounded by vine plots infested by this species. Number of crawlers collected on traps was generally higher on the trap side exposed downwind and depended on population density of neighbouring vines. Coloured glitters of size and weight similar to that of crawlers were used to simulate wind transport. The highest number of glitters was trapped at the prevailing wind sector. A majority of glitters was found on traps close to dispersion cups, and the most remote were collected up to 165 m away from glitter source. Wind dispersal of crawlers was confirmed by colonisation of the new vine plot by P. corni, 2 years after plantation. The settling of P. corni on the young plot displayed no significant structure, as expected from a wind dispersal. Although grapevine leafroll viruses and Grapevine virus A were detected by reverse transcription-polymerase chain reaction in several batches of trapped nymphs, these viruses were not found in vines colonised by P. corni in the new plot within the 4 years following plantation.

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“…Like all members of the family Closteroviridae, GLRaV-1 is a phloem-restricted virus that is grafttransmissible. GLRaV-1 and other leafroll ampeloviruses are naturally spread in vineyards by several mealybugs and soft scales [6]. Grapevine virus A (GVA, genus Vitivirus, family Betaflexiviridae) is associated with 'Kober stem grooving syndrome', one of the 'rugose wood complex' disorders, which is considered a secondary disease of grapevine [13].…”

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confidence: 99%

“…Grapevines with leafroll are frequently coinfected with phloem-limited vitiviruses, such as GVA [18], which are also transmissible by mealybugs and soft scales. Moreover, since vitiviruses are frequently transmitted along with GLRaVs, the hypothesis that the ampelovirus may assist the coinfecting vitivirus during transmission has been raised [6,7,14,21] and has generated an active debate within the grapevine entomology and virology community [6,18]. For this work, viral particles were purified from 80 g of P70 accession leaves sampled in greenhouses early in April 2014, following a previously described protocol [1].…”

mentioning

confidence: 99%

Virus preparations from the mixed-infected P70 Pinot Noir accession exhibit GLRaV-1/GVA ‘end-to-end’ particles

Alliaume¹,

Reinbold²,

Erhardt³

et al. 2018

Arch Virol

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P70 is a Pinot Noir grapevine accession that displays strong leafroll disease symptoms. A high-throughput sequencing (HTS)-based analysis established that P70 was mixed-infected by two variants of grapevine leafroll-associated virus 1 (GLRaV-1, genus Ampelovirus) and one of grapevine virus A (GVA, genus Vitivirus) as well as by two viroids (hop stunt viroid [HSVd] and grapevine yellow speckle viroid 1 [GYSVd1]) and four variants of grapevine rupestris stem pitting-associated virus (GRSPaV). Immunogold labelling using gold particles of two different diameters revealed the existence of 'hybrid' particles labelled at one end as GLRaV-1, with the rest labelled as GVA. In this work, we suggest that immunogold labelling can provide information about the biology of the viruses, going deeper than just genomic information provided by HTS, from which no recombinant or 'chimeric' GLRaV-1/GVA sequences had been identified in the dataset. Our observations suggest an unknown interaction between members of two different viral species that are often encountered together in a single grapevine, highlighting potential consequences in the vector biology and epidemiology of leafroll and rugose-wood diseases.

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Vector Transmission of Grapevine Leafroll-Associated Viruses

Cited by 39 publications

References 69 publications

A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.

A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.

Gone with the wind: Aerial dispersal of Parthenolecanium corni crawlers in a newly planted grapevine plot

Virus preparations from the mixed-infected P70 Pinot Noir accession exhibit GLRaV-1/GVA ‘end-to-end’ particles

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