Virus preparations from the mixed-infected P70 Pinot Noir accession exhibit GLRaV-1/GVA ‘end-to-end’ particles

Alliaume, Antoine; Reinbold, Catherine; Erhardt, Mathieu; Beuve, Monique; Hily, Jean-Michel; Lemaire, Olivier; Herrbach, Étienne

doi:10.1007/s00705-018-3995-3

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…The clearly visible phenotypic characteristics of infection were a darker area around the leaf veins and downward-twisted leaf edges (Martelli 1993, Andret-Link et al 2004, Meng et al 2017. Both symptoms are characteristic of red varieties infected with the most widespread and damaging viruses (Alliaume et al 2018, Sabella et al 2018 of the leafroll complex (Meng et al 2017). In the advanced phase of infection, the viruses of the leafroll complex cause anthocyanin accumulation.…”

Section: Sanitary Status Of Field-grown Plants Used As Explant Donorsmentioning

confidence: 99%

Somatic embryogenesis as a tool for virus elimination in Croatian indigenous grapevine cultivars

Malenica

Jagić

Pavletić

et al. 2020

Acta bot. Croat. (Online)

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Most Croatian indigenous grapevine cultivars and vineyards are infected with a few dominant viruses. The goal of this study was to establish somatic embryogenesis as an efficient method for virus elimination from valuable Croatian cultivars and creating a reliable source of healthy plants. Somatic embryogenesis was induced from immature anthers and somatic embryogenesis-derived plantlets for seven indigenous cultivars were successfully regenerated. This procedure led to the elimination of viruses GFLV, GLRaV-1, GLRaV-3 and GFkV that were initially detected in the field-grown cultivars ‘Plavac mali’ and ‘Babica’ with an elimination success of at least 30%. The described method has the potential for production of virus-free rooted plantlets for all economically important cultivars or superior cultivar clones and for the establishment of a steady source of certified virus-free planting material.

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Section: Sanitary Status Of Field-grown Plants Used As Explant Donorsmentioning

confidence: 99%

Somatic embryogenesis as a tool for virus elimination in Croatian indigenous grapevine cultivars

Malenica

Jagić

Pavletić

et al. 2020

Acta bot. Croat. (Online)

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“…Virus source grapevines were rooted cuttings from the following accessions of our reference collection of grapevine viruses [ 18 ]: P70 [ 19 , 20 ], Y199, Y217, Y252, Y258, Y318, and Cab119-66. Their virus content was checked by ELISA (see Section 2.5 below) and RT-PCR, as previously detailed in [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Transmission of Grapevine leafroll-associated virus-1 (Ampelovirus) and Grapevine virus A (Vitivirus) by the Cottony Grape Scale, Pulvinaria vitis (Hemiptera: Coccidae)

Hommay

Alliaume²,

Reinbold

et al. 2021

Viruses

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The cottony grape scale Pulvinaria vitis is a scale insect colonizing grapevine; however, its capacity as a vector of grapevine viruses is poorly known in comparison to other scale species that are vectors of viral species in the genera Ampelovirus and Vitivirus. The ability of P. vitis to transmit the ampeloviruses Grapevine leafroll-associated viruses [GLRaV]−1, −3, and −4, and the vitivirus Grapevine virus A (GVA), to healthy vine cuttings was assessed. The scale insects used originated from commercial vine plots located in Alsace, Eastern France. When nymphs sampled from leafroll-infected vineyard plants were transferred onto healthy cuttings, only one event of transmission was obtained. However, when laboratory-reared, non-viruliferous nymphs were allowed to acquire viruses under controlled conditions, both first and second instar nymphs derived from two vineyards were able to transmit GLRaV−1 and GVA. This is the first report of GLRaV−1 and GVA transmission from grapevine to grapevine by this species.

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“…ficus [9], Pseudococcus longispinus (Targioni Tozzetti) [10] and Parthenolecanium corni (Bouché) [11]. As these vitiviruses are frequently transmitted along with GLRaV 1 or 3, the hypothesis that ampeloviruses may assist vitiviruses during transmission has been raised [7,[11][12][13][14]. A semi-persistent transmission mode is believed to apply to soft scales, as already shown for mealybugs (e.g., [7,10,[15][16][17][18][19][20]).…”

Section: Introductionmentioning

confidence: 99%

Transmission of Grapevine Leafroll-Associated Viruses and Grapevine Virus A by Vineyard-Sampled Soft Scales (Parthenolecanium corni, Hemiptera: Coccidae)

Hommay

Beuve

Herrbach

2022

Viruses

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Grapevine-infecting ampelo- and vitiviruses are transmitted by scale insects belonging to several species, among which is the European fruit lecanium, Parthenolecanium corni (Bouché) (Hemiptera Coccidae). Our objective was to characterize the transmission biology of grapevine leafroll-associated viruses (GLRaV) and grapevine virus A (GVA) by this soft scale species in order to evaluate its ability to spread these viruses. In transmission experiments with nymphs sampled from different vineyards infected with GLRaV 1, 2, 3 and GVA, P. corni transmitted only GLRaV 1 and GVA to healthy vines. GVA was predominantly transmitted along with GLRaV 1, whereas the latter could be transmitted alone from single or co-infected vines. Vineyard-sampled second instar nymphs were more efficient than first instars at transmitting GLRaV 1, whereas both instars displayed similar transmission rates for GVA. Short virus inoculation access periods and the absence of virus in eggs of females living on infected grapevines fulfilled the criteria of non-circulative semi-persistent transmission mode.

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Virus preparations from the mixed-infected P70 Pinot Noir accession exhibit GLRaV-1/GVA ‘end-to-end’ particles

Cited by 5 publications

References 18 publications

Somatic embryogenesis as a tool for virus elimination in Croatian indigenous grapevine cultivars

Somatic embryogenesis as a tool for virus elimination in Croatian indigenous grapevine cultivars

Transmission of Grapevine leafroll-associated virus-1 (Ampelovirus) and Grapevine virus A (Vitivirus) by the Cottony Grape Scale, Pulvinaria vitis (Hemiptera: Coccidae)

Transmission of Grapevine Leafroll-Associated Viruses and Grapevine Virus A by Vineyard-Sampled Soft Scales (Parthenolecanium corni, Hemiptera: Coccidae)

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