Vegetative Compatibility Grouping of Colletotrichum kahawae in Kenya

Gichuru, E. K.; Várzea, Vítor; Rodrigues, Carlos; Masaba, D. M.

doi:10.1046/j.1439-0434.2000.00098.x

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…Complementation also occurred in some pairings between nit 1 and nit 3 mutants (Katan and Shabi, 1996). Mutants of the same phenotype (especially nit 1 mutants) were occasionally observed to complement both within and between isolates (intragenic complementation), as has been reported in similar studies (Correll et al., 1987; Katan and Shabi, 1996; Gichuru et al., 2000; Varzea et al., 2002).…”

Section: Resultssupporting

confidence: 64%

“…Vegetative compatibility (VC) is another effective approach for studying the genetic structure of C. gloeosporioides populations on yam, as has been demonstrated in Colletotrichum diseases of many crops (Correll et al., 1993; Katan and Shabi, 1996; Freeman et al., 1998; Gichuru et al., 2000; Varzea et al., 2002). Studies on the genetic control of VC in C. gloeosporioides indicate that VC is controlled by three to seven vic loci (Correll et al., 2000).…”

Section: Introductionmentioning

confidence: 99%

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Vegetative Compatibility Among Isolates of Colletotrichum gloeosporioides from Yam (Dioscorea spp.) in Nigeria

Abang¹,

Hoffmann²,

Winter³

et al. 2004

Journal of Phytopathology

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Isolates of Colletotrichum gloeosporioides obtained from yam‐based cropping systems in Nigeria, previously characterized on the basis of morphology, virulence and rDNA internal transcribed spacer (ITS) sequence variation were further compared for vegetative compatibility (VC). Chlorate‐resistant nitrate non‐utilizing (nit) mutants were generated from the isolates and used in complementation (heterokaryon) tests. Tests of VC between complementary mutants from different isolates indicated the presence of several genotypes within a single field, suggesting limited clonal spread. In some cases, isolates obtained from the same lesion were observed to belong to different vegetative compatibility groups (VCGs). No compatibility was observed between isolates of the highly virulent slow‐growing grey (SGG), the moderately virulent fast‐growing salmon (FGS) and the avirulent/weakly virulent fast‐growing grey (FGG) strains. Forty‐one C. gloeosporioides isolates belonged to 28 VCGs, giving a genotype diversity estimate of 0.68. This diversity confirmed the high variability of the pathogen population as revealed by previous characterization studies, however, a correlation between VCGs and isolate groupings based on morphology and virulence was not found. The finding that an isolate from weed was compatible with yam isolates indicated that transfer of important traits, such as virulence, may take place between isolates from yam and non‐yam hosts. The VCG diversity revealed by this study suggests that in addition to asexual reproduction, sexual reproduction may play an important role in the epidemiology of anthracnose on yam.

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Section: Resultssupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Vegetative Compatibility Among Isolates of Colletotrichum gloeosporioides from Yam (Dioscorea spp.) in Nigeria

Abang¹,

Hoffmann²,

Winter³

et al. 2004

Journal of Phytopathology

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“…The characterization of VCGs has been employed successfully in various species of Colletotrichum (Brooker et al, 1991;Skroch et al, 1992;Wasilwa et al, 1993;Correll et al, 1993;Chacko et al, 1994;Beynon et al, 1995;Gichuru et al, 2000;Nitzan et al, 2002;Varzea et al, 2002;Abang et al, 2004;Pereira, 2005), and nitrate non-utilizing mutants have been used to demonstrate heterokaryosis and parasexual segregation in C. lindemuthianum isolates (Castro-Prado et al, 2007). However, little could be inferred about vegetative compatibility in this species, because the nit mutants were obtained from only five isolates.…”

Section: Introductionmentioning

confidence: 99%

Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil

Barcelos¹,

Souza²,

Silva³

2011

Genet. Mol. Res.

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ABSTRACT. The causal agent of common bean anthracnose, Colletotrichum lindemuthianum, has considerable genetic and pathogenic variability, which makes the development of resistant cultivars difficult. We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibility groups (VCGs) and by RAPD analysis. Two hundred and ninety-five nit mutants were obtained from 47 isolates of various pathotypes of the fungus collected from different regions, host cultivars and years. In complementation tests, 45 VCGs were identified. Eighteen RAPD primers were employed in the molecular analyses, producing 111 polymorphic bands. Estimates of genetic similarities, determined from the Sorence-Dice coefficient, ranged from 0.42 to 0.97; the dendrogram obtained by cluster analysis revealed 18 groups of isolates. RAPD and VCG markers presented high genotypic diversity. The number of significant associations (P = 0.05) between RAPD, VCG and pathogenicity markers ranged from 0 (VCG) to 80% (pathogenicity). The test of multilocus association (r d ) for RAPD markers was significantly different from zero (P < 0.001), suggesting linkage disequilibrium. However, the results for VCG markers show the presence of recombination mechanisms. In conclusion, RAPD markers and VCGs were useful for detecting genetic variability among isolates of C. lindemuthianum. We found considerable diversity among isolates from the same geographic origin within a short interval; this suggests rapid evolution. There is a need for further studies to elucidate the population structure of this pathogen in agro-ecosystems.

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“…These two isolates also differed in their VCG reactions (Beynon et al., 1995). Gichuru et al. (2000) found that 44 East African isolates of C. kahawae (42 from Kenya, one from Ethiopia and one from Malawi) all belonged to the same VCG, except for one white sector mutant.…”

Section: Discussionmentioning

confidence: 97%

Variability of Colletotrichum kahawae in Relation to Other Colletotrichum Species from Tropical Perennial Crops and the Development of Diagnostic Techniques

Bridge¹,

Waller²,

Davies³

et al. 2008

Journal of Phytopathology

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Twenty-six isolates of Colletotrichum kahawae, the causal agent of coffee berry disease, from coffee in Africa, and 25 isolates, mostly of Colletotrichum gloeosporioides, from coffee and other tropical perennial crops, were examined for the ability to metabolize citrate and tartrate and their molecular genetic variability was assessed using restriction fragment length polymorphisms (RFLP) and variable number tandem repeats (VNTR). Twenty-four isolates of C. kahawae were also assessed using amplified fragment length polymorphisms (AFLP). Vegetative compatibility within a collection of nine isolates, including two of C. gloeosporioides was also assessed. All isolates of C. kahawae from across Africa failed to metabolize citrate or tartrate, but all other isolates metabolized one or both. Colletotrichum kahawae isolates also showed minimal variability using the molecular techniques with two isolates from Cameroon showing slightly different banding patterns in RFLP analysis. All other isolates had variable VNTR and RFLP banding patterns. AFLP analysis failed to detect variability within 12 isolates from Kenya, but did detect differences between isolates from other countries. Five isolates from Kenya were vegetatively compatible but differed from two from Cameroon and from two C. gloeosporioides isolates. Results demonstrate some geographic variability within C. kahawae isolates, although this is small, probably due to the relatively young age of C. kahawae populations. The biochemical and molecular techniques used showed clear differences from other Colletotrichum isolates, and can be used to distinguish the species. Lack of citrate and tartrate metabolism provides a readily applicable diagnostic method.

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Vegetative Compatibility Grouping of Colletotrichum kahawae in Kenya

Cited by 5 publications

References 18 publications

Vegetative Compatibility Among Isolates of Colletotrichum gloeosporioides from Yam (Dioscorea spp.) in Nigeria

Vegetative Compatibility Among Isolates of Colletotrichum gloeosporioides from Yam (Dioscorea spp.) in Nigeria

Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil

Variability of Colletotrichum kahawae in Relation to Other Colletotrichum Species from Tropical Perennial Crops and the Development of Diagnostic Techniques

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