Vegetative propagation of sandalwood plants through tissue culture

Abstract

Hypocotyl segments excised from seedlings of Santalum album (sandalwood tree) grown in vitro were cultured aseptically on various nutrient media. Regeneration of shoot buds on explants was observed on basal media supplemented with auxins like IAA, IBA, NAA, or NOA. Shoot bud formation was completely suppressed on 2,4-D or pCPA medium. Cytokinins stimulated and enhanced shoot bud formation better than auxins. Excised stem segments from adult plants did not respond to any hormonal treatment. The importance of this method in the propagation of the sandalwood tree is emphasized.

In vitro clonal propagation of Clerodendrum serratum (Linn.) Moon (barangi): a rare and threatened medicinal plant

“…For induction and multiplication of shoots from various explants of woody species, superiority of BAP over other cytokinins has already been established (Murashige 1974;Chaturvedi et al 2004). The usefulness of AdS for improved multiplication of shoots is well documented by many workers (Rao and Bapat 1978;Reuveni et al 1990). …”

Tissue culture of parasitic flowering plants: Methods and applications in agriculture and forestry

“…The tree is difficult to propagate by vegetative means, such as grafting and rooting cuttings, and trees over 3 yr old are prone to spike disease, a destructive untreatable disease caused by phytoplasma-like organisms (112). Seeds are viable for 1 yr, and seedlings typically have a high mortality rate.…”

In vitro plant regeneration from nodal segments of the spontaneous F1 hybrid Santalum yasi × S. album and its parents S. album and S. yasi

“…The optimum medium for shoot multiplication was MS medium supplemented with BA and NAA while embryogenic callus-mediated SE formation was an efficient means of producing SEs in S. yasi 9 S. album and S. album using a cell suspension culture. In previous S. album studies, stem nodal segments were optimal explants to induce adventitious shoots on MS medium supplemented with BA and NAA (Rao and Bapta 1978;Bapat and Rao 1984;Rao and Bapat 1993;Muthan et al 2006;Sanjaya et al 2006). High-frequency shoot proliferation from the internodes of S. album was possible on MS medium supplemented with 4.92 lM 2-isopentenyl adenine (2iP) and 10 % coconut milk (Janarthanam and Sumathi 2011).…”

“…For S. album, however, successful protocols exist for the induction of adventitious shoots from young shoots (Lakshmi Sita et al 1979;Rao and Raghava Ram 1983), nodal shoot segments Bapta 1978, 1993;Rugkhla and Jones 1998;Sanjaya et al 2006;Janarthanam andSumathi 2011), zygotic embryos (Ravishankar and, endosperm (Rangaswamy and Rao 1963), and leaves (Mujib 2005;Bele et al 2012;Singh et al 2013). Most of these studies used Murashige and Skoog (1962) (MS) basal medium to induce and proliferate shoots and an optimized combination of 6-benzyladenine (BA) and a-naphthaleneacetic acid (NAA) on MS medium resulted in the induction of adventitious shoots in S. album (Rao and Bapta 1978;Bapat and Rao 1984;Rao and Bapat 1993;Muthan et al 2006;Sanjaya et al 2006).…”