Velocity control of nanoliter droplets using a pneumatic dispensing system

Lee, Sang‐Min; Choi, In Ho; Kim, Young Kwon; Kim, Joonwon

doi:10.1186/s40486-014-0005-8

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…This difference in viability may have occurred because the higher droplet velocity subjected dispensed cells to a larger shear stress than those experienced by cells in the pipetted control. For the current design of the dispenser, the measured droplet velocities were in range from 1.6 to 5.4 m/s while applied pressures were changed [24], which are higher than typical inkjet printing system such as piezoelectric or thermal bubble jet [25].…”

Section: Cell Viabilitymentioning

confidence: 99%

Feasibility study of a biocompatible pneumatic dispensing system using mouse 3T3-J2 fibroblasts

Lee

Kim

2017

Micro and Nano Syst Lett

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This paper presents results for dispensing living cells using a pneumatic dispensing system to verify the feasibility of using this system to fabricate biomaterials. Living cells (i.e., mouse 3T3-J2 fibroblast) were dispensed with different dispensing pressures in order to evaluate the effect of dispensing process on cell viability and proliferation. Based on the results of a live-dead assay, more than 80% of cell viability has been confirmed which was reasonably similar to that in the control group. Furthermore, measurement of cell metabolic activity after dispensing confirmed that the dispensed cell proliferated at a rate comparable to that of the control group. These results demonstrate that the pneumatic dispensing system is a promising tool for fabrication of biomaterials.

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Section: Cell Viabilitymentioning

confidence: 99%

Feasibility study of a biocompatible pneumatic dispensing system using mouse 3T3-J2 fibroblasts

Lee

Kim

2017

Micro and Nano Syst Lett

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“…In this study, we introduced a pneumatic dispenser [10][11][12] that can monitor the droplet ejection status in real-time using a capacitive-type sensor embedded on a flexible membrane using pneumatic pressure deformation. The difference between the membrane deformation under normal and abnormal operating conditions was…”

Section: Introductionmentioning

confidence: 99%

A 3D-printed pneumatic dispenser with monitoring droplet ejection

Kang

Park

Lee

2022

Micro and Nano Syst Lett

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In this study, a pneumatic dispenser driven by a flexible membrane with a capacitive-type sensor using an SLA-type 3D printer was fabricated. It was confirmed that a single droplet in the range of approximately 400–450 nL could be ejected from the current processed 200-μm-diameter nozzle. The deformation varied according to the magnitude and time of the positive pressure applied to the membrane sensor. In addition, the signals of the normal dispensing and abnormal states, in which the solution was not ejected when the inlet pressure was removed, were measured and compared. The base capacitance-to-digital converter (CDC) value decreased when the inlet pressure was removed. Thus, it was able to confirm the feasibility of monitoring the normal and abnormal ejection status of the pneumatic dispenser.

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“…Valve-based dispensing systems composed of a high-pressure chamber, pump, and valve controller are commonly used in noncontact-type systems. The liquid inside the chamber is subjected to a high pressure, and the valve is opened or closed in a short time by a piezoelectric device [15,16] or a solenoid [17,18] in order to dispense the droplets. This technology can dispense small volumes with precision; however, the dispensing process is affected by the experimental environment, including the temperature.…”

Section: Introductionmentioning

confidence: 99%