Velocity map imaging mass spectrometry

Kim, Myung Hwa; Leskiw, Brian D.; Shen, Lei; Suits, Arthur G.

doi:10.1016/j.ijms.2006.01.030

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…However, in microscope‐mode imaging the trajectories of the analyte ions have to be controlled, which severely limits the range of techniques that can be used to increase the mass resolution of an experiment. Methods common to microprobe‐mode MS, such as the use of reflectrons, or ion cyclotron resonance instrumentation, allow for mass resolution ( m /Δ m 50 % ) of the order of 10 5 , but have not been shown to be compatible with microscope‐mode imaging; however, Suits and co‐workers have shown well‐resolved velocity‐mapped images (those associated with the back‐focal plane of the imaging system) using an instrument with a single‐stage reflectron implying that spatial imaging should be possible . It has also been shown that electrostatic analysers (ESAs) can be used to improve focusing in the time domain while retaining spatial information in microscope mode.…”

mentioning

confidence: 99%

Modifications to a commercially available linear mass spectrometer for mass‐resolved microscopy with the pixel imaging mass spectrometry (PImMS) camera

Halford

Winter

Mills³

et al. 2014

Rapid Comm Mass Spectrometry

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mentioning

confidence: 99%

Modifications to a commercially available linear mass spectrometer for mass‐resolved microscopy with the pixel imaging mass spectrometry (PImMS) camera

Halford

Winter

Mills³

et al. 2014

Rapid Comm Mass Spectrometry

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“…The experimental apparatus used in this work was an adapted conventional velocity-map [7] ion imaging [8] instrument. The apparatus has been described in detail previously, both with [9] and without [10,11] the fast-framing camera, and only the most recent modifications to the apparatus shall be described here.…”

Section: Methodsmentioning

confidence: 99%

Application of fast sensors to microscope mode spatial imaging mass spectrometry

Brouard

Johnsen

Nomerotski³

et al. 2011

J. Inst.

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The application of fast detectors to microscope mode spatial imaging mass spectrometry, in which the two-dimensional distributions of particular ion masses over a sample are projected onto a two-dimensional detector, allows the imaging of all mass peaks desorbed from a sample on each time-of-flight cycle. Detecting all fragments per duty cycle means that fewer ionisation and acquisition cycles are required for a full set of data, leading to a reduction in overall detection time, amount of sample required, and sample degradation. Results from a proof-of-concept experiment, in which a fast frame transfer charge-coupled device camera (CCD) with 10 ns time resolution was coupled to a modified velocity-mapped imaging apparatus, are presented. KEYWORDS: Pixelated detectors and associated VLSI electronics; Instrumentation and methods for time-of-flight (TOF) spectroscopy; Mass spectrometers 3 Results and discussion 4 3.1 Results 4 3.2 Resolution determination and aberrations 4 4 Conclusions 6

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“…14,31 To enhance detection efficiency, mass resolving power and mass accuracy of MS, various technologies (in ionisation methods, mass analysers and detectors) have been developed for different applications and for use in multi-MS platforms. 14,[40][41][42][43] In plant metabolomic analyses, MS is the key platform for compound identification, an essential step in the workflow of any metabolomic analysis. The mass spectral data provides a pattern that is most often compound specific.…”

Section: And Weckwerth and Morgenthal 32mentioning

confidence: 99%

Plant metabolomics: A new frontier in phytochemical analysis

2013

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The primary and secondary metabolites found in plant cells are the final recipients of biological information flow. In turn, their levels can influence gene expression and protein stability. Qualitative and quantitative measurements of these metabolites reflect the cellular state under defined conditions, and yield critical insights into the cellular processes that control the biochemical phenotype of the cell, tissue or whole organism. Metabolomics differs from traditional targeted phytochemical analysis in various fundamental aspects; for example, it is a data-driven approach with predictive power that aims to assess all measurable metabolites without any pre-conception or pre-selection. As such, metabolomics is providing new dimensions in the study of systems biology, enabling the in-depth understanding of the intra-and extracellular interactions of plant cells. Metabolomics is also developing into a valuable tool that can be used to monitor and assess gene function, and to characterise post-genomic processes from a broad perspective. Here, we give an overview of the fundamental analytical technologies and subsequent multivariate data analyses involved in plant metabolomics as a research tool to study various aspects of plant biology.

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Velocity map imaging mass spectrometry

Cited by 12 publications

References 21 publications

Modifications to a commercially available linear mass spectrometer for mass‐resolved microscopy with the pixel imaging mass spectrometry (PImMS) camera

Modifications to a commercially available linear mass spectrometer for mass‐resolved microscopy with the pixel imaging mass spectrometry (PImMS) camera

Application of fast sensors to microscope mode spatial imaging mass spectrometry

Plant metabolomics: A new frontier in phytochemical analysis

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