Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining

Hussein, Elawad A.; Hair-Bejo, M.; Omar, Abdul Rahman; Arshad, Siti Suri; Hani, Homayoun; Balakrishnan, Krishnan Nair; Yakubu, Yusuf; Saeed, Mohammed Ibrahim; Ideris, Aini

doi:10.1016/j.micpath.2019.02.017

Cited by 15 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The brain when affected by ND shows neurological symptoms that cause uncoordinated movements [ 14 ]. In both the lung and digestive tract, ND infection causes severe bleeding along with pneumonia [ 15 ] and necrosis of the intestine that inhibits nutrient absorption and manipulates the activation of intestinal intraepithelial natural killer cells (IEL-NK) [ 16 ]. Inside the lymphoid system, the ND virus destroys lymphocytes and inhibits antibody formation, leading to decreased success of vaccination [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

et al. 2020

View full text Add to dashboard Cite

Background and Aim: Newcastle disease (ND) is a viral infection that causes high mortality and economic loss in the poultry industry. The Office International des Epizooties (OIE) recommends several diagnostic methods for the detection of ND, including isolation and molecular tests. However, these detection methods are time-consuming and highly expensive. Therefore, this study was conducted to develop a coagglutination kit as a novel diagnostic tool for ND in the poultry industry. Materials and Methods: Two adult male New Zealand White rabbits weighing 2.5 kg were vaccinated using ND life vaccine intraperitoneally. The vaccination was conducted once a week for 4 weeks with multilevel doses. Rabbits' serum was collected at week 6 and inactivated at 56°C for 30 min. The serum was precipitated using ammonium sulfate and reacted with protein A of Staphylococcus aureus to produce the agglutination kit for detecting ND virus. A total of 25 chickens suspected with ND infection from a local poultry farm in Yogyakarta were used as the test samples. The chickens were necropsied, and the brain, spleen, lung, intestine, and feces were collected. Half of these organs were subjected to tests using the coagglutination kit and reverse transcription-polymerase chain reaction (RT-PCR). The other half was processed for histopathology. Data were analyzed qualitatively. Results: Of the 25 samples, 13 (52%) were positive for ND infection when tested using both the ND coagglutination kit and RT-PCR. The positive samples also exhibited several histopathological changes, including perivascular cuffing surrounding the cerebral blood-brain barrier, hemorrhagic pneumonia, splenitis, and necrotic hemorrhage enteritis. Conclusion: This study confirmed that the ND coagglutination kit could be used as a novel diagnostic tool for the detection of ND virus infection in the poultry industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The scalding, defeathering, evisceration, and chilling processes were identified as responsible for substantial cross-contamination during typically automated chicken slaughter (Chen et al, 2020). Furthermore, HE staining is a mature histopathological method used for visually observing the inner microstructural changes of tissues (Hussein et al, 2019). Mini punch is a tissue transplantation and biopsy sampling method widely used during medical skin surgery (Huang et al, 2012;Hirobe and Enami, 2018;Fofanov et al, 2019), and it can be combined with other analytical methods to realize the detection and analysis of trace samples.…”

Section: Introductionmentioning

confidence: 99%

Complex Internal Microstructure of Feather Follicles on Chicken Skin Promotes the Bacterial Cross-Contamination of Carcasses During the Slaughtering Process

Zhang

Peng

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

“…In the present study, we investigated the effects of NDV infection on the expression of PRRs, innate immune genes, enzymes involved in steroidogenesis, reproduction hormones levels, and histopathology of testes in SPF roosters. Many studies have attempted to explain the modulation of innate immune genes and histological structure in different organs and organ systems, including the digestive system, respiratory system, spleen, nervous system and female reproductive system [14,[24][25][26][27][28]. However, to the best of our knowledge, an attempt has never been made to study the fate of NDV infection in the testes of roosters.…”

Section: Discussionmentioning

confidence: 99%

“…In poultry, studies of NDV pathogenesis have mainly focused on the intestines, lungs, trachea, brain, spleen and feathers [14,[24][25][26][27][28][29]. Studies on the effects of NDV infection on histological lesions, innate immune responses, and steroidogenesis in the testes are rare.…”

Section: Introductionmentioning

confidence: 99%

Newcastle disease virus induces testicular damage and disrupts steroidogenesis in specific pathogen free roosters

Rehman

Ren

Yang

et al. 2020

Vet Res

View full text Add to dashboard Cite

Newcastle disease (ND), which is caused by Newcastle disease virus (NDV), can cause heavy economic losses to the poultry industry worldwide. It is characterised by extensive pathologies of the digestive, respiratory, and nervous systems and can cause severe damage to the reproductive system of egg-laying hens. However, it is unknown whether NDV replicates in the male reproductive system of chickens and induces any pathologies. In this study, we selected a representative strain (i.e. ZJ1) of the most common genotype (i.e. VII) of NDV to investigate whether NDV can induce histological, hormonal, and inflammatory responses in the testes of specific pathogen free (SPF) roosters. NDV infection increased the expression of toll like receptor TLR3, TLR7, MDA5, IFN-α, IFN-β, IFN-γ, IL-8, and CXCLi1 in the testes of NDV-infected roosters at 5 days post-infection (dpi). Severe histological changes, including decrease in the number of Sertoli cells and individualized, shrunken spermatogonia with pyknotic nuclei, were observed at 3 dpi. At 5 dpi, the spermatogenic columns were disorganized, and there were fewer cells, which were replaced by necrotic cells, lipid vacuoles, and proteinaceous homogenous material. A significant decrease in the plasma concentrations of testosterone and luteinizing hormone (LH) and the mRNA expression of their receptors in the testes, steroidogenic acute regulatory protein, cytochrome P450 side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase in the NDVinfected group was observed relative to those in the control group (P < 0.05). Collectively, these results indicate that NDV infection induces a severe inflammatory response and histological changes, which decrease the steroidogenesis.

show abstract

Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining

Cited by 15 publications

References 19 publications

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

Development of a coagglutination kit as a rapid test for diagnosing Newcastle disease in poultry

Complex Internal Microstructure of Feather Follicles on Chicken Skin Promotes the Bacterial Cross-Contamination of Carcasses During the Slaughtering Process

Newcastle disease virus induces testicular damage and disrupts steroidogenesis in specific pathogen free roosters

Contact Info

Product

Resources

About