Venom gland transcriptome from Heloderma horridum horridum by high-throughput sequencing

Lino-López, Gisela Jareth; Valdez-Velázquez, Laura Leticia; Corzo, Gerardo; Romero-Gutiérrez, Teresa; Jiménez-Vargas, Juana María; Rodríguez-Vázquez, Armando; Vázquez‐Vuelvas, Oscar F.; Gonzalez-Carrillo, Gabino

doi:10.1016/j.toxicon.2020.04.003

Cited by 8 publications

(20 citation statements)

References 97 publications

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“…In the present study, we obtained 882,273 CCSs, identified 746,317 FLNC, and then yielded 212,964 corrected isoforms with an average read length of 4,153 bp. Compared with short-read sequencing (e.g., Illumina sequencing), the mean length of SMRT-sequenced transcripts was greater than 3 kb, which far exceeded the value reported in previous studies analyzing Heloderma horridum horridum [32], Gekko japonicas [33], Palaemon serratus [34], and Henosepilachna vigintioctopunctata [35]. Furthermore, 117,888 high-quality unique full-length transcripts were generated based on the high competence of PacBio SMRT sequencing, and 111,372 transcripts were successfully annotated with 116,913 ORFs.…”

Section: Plos Onementioning

confidence: 53%

SMRT sequencing of the full-length transcriptome of Gekko gecko

et al. 2022

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Tokay Gecko (Gekko gecko) is a rare and endangered medicinal animal in China. Its dry body has been used as an anti-asthmatic agent for two thousand years. To date, the genome and transcriptome of this species remain poorly understood. Here, we adopted single molecule real-time (SMRT) sequencing to obtain full-length transcriptome data and characterized the transcriptome structure. We identified 882,273 circular consensus (CCS) reads, including 746,317 full-length nonchimeric (FLNC) reads. The transcript cluster analysis revealed 212,964 consensus sequences, including 203,994 high-quality isoforms. In total, 111,372 of 117,888 transcripts were successfully annotated against eight databases (Nr, eggNOG, Swiss-Prot, GO, COG, KOG, Pfam and KEGG). Furthermore, 23,877 alternative splicing events, 169,128 simple sequence repeats (SSRs), 10,437 lncRNAs and 7,932 transcription factors were predicted across all transcripts. To our knowledge, this report is the first to document the G. gecko transcriptome using SMRT sequencing. The full-length transcript data might accelerate transcriptome research and lay the foundation for further research on G. gecko.

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Section: Plos Onementioning

confidence: 53%

SMRT sequencing of the full-length transcriptome of Gekko gecko

et al. 2022

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“…Transcriptomic analyses using Sanger cDNA libraries have been reported, 7,8 and recently our group reported the RNA-seq transcriptome from Heloderma horridum horridum venom glands, identifying 199 transcripts categorized into five protein groups: enzymes, host defense proteins, proteins inhibitors, toxins, and other component proteins, being exendins the component with the major expression in relative abundance. 9 Also, we found novel transcripts for translationally controlled tumor proteins, venom factors, vespryns, waprins, lectins, cystatins, serine protease inhibitors, and others. 9 Relatedly, proteomic approaches were performed by Koludarov et al 10 and Sanggaard et al 11 in Helodermatidae species (H. horridum and H. suspectrum) focusing on 2-DE.…”

Section: ■ Introductionmentioning

confidence: 83%

Proteomic Analysis of Heloderma horridum horridum Venom: Assessment to Its Transcriptome and Newfound Proteins

Lino-López,

Ruiz-May,

Elizalde-Contreras

et al. 2024

J. Proteome Res.

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Heloderma horridum horridum, a venomous reptile native to America, has a venom with potential applications in treating type II diabetes. In this work, H. h. horridum venom was extracted, lyophilized, and characterized using enzymatic assays for hyaluronidase, phospholipase, and protease. Proteomic analysis of the venom was conducted employing bottom-up/shotgun approaches, SDS-PAGE, high-pH reversedphase chromatography, and fractionation of tryptic peptides using nano-LC-MS/MS. The proteins found in H. h. horridum venom were reviewed according to the classification of the transcriptome previously reported. The proteomic approach identified 101 enzymes, 36 other proteins, 15 protein inhibitors, 11 host defense proteins, and 1 toxin, including novel venom components such as calcium-binding proteins, phospholipase A2 inhibitors, serpins, cathepsin, subtilases, carboxypeptidase-like, aminopeptidases, glycoside hydrolases, thioredoxin transferases, acid ceramidase-like, enolase, multicopper oxidases, phosphoglucose isomerase (PGI), fructose-1,6-bisphosphatase class 1, pentraxin-related, peptidylglycine α-hydroxylating monooxygenase/peptidyl-hydroxyglycine α-amidating lyase, carbonic anhydrase, acetylcholinesterase, dipeptidylpeptidase, and lysozymes. These findings contribute to understanding the venomous nature of H. h. horridum and highlight its potential as a source of bioactive compounds. Data are available via PRoteomeXchange with the identifier PXD052417.

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“…Recent works allowed access to different classes of proteins, and also new biological activities from Heloderma venom. The venom gland transcriptome analysis from H. horridum horridum revealed the presence of metalloproteases, lipases, vespryns, waspryns, lectins, cystatins and serine peptidase inhibitors, but none of these proteins were actually isolated from the venom [71]. Furthermore, Heloderma contains neurotoxins in its venom, and these toxins are able to bind sodium and calcium channels [72].…”

Section: Lizardsmentioning

confidence: 99%

Neglected Venomous Animals and Toxins: Underrated Biotechnological Tools in Drug Development

Coelho

Silva

Beraldo-Neto

et al. 2021

Toxins

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Among the vast repertoire of animal toxins and venoms selected by nature and evolution, mankind opted to devote its scientific attention—during the last century—to a restricted group of animals, leaving a myriad of toxic creatures aside. There are several underlying and justifiable reasons for this, which include dealing with the public health problems caused by envenoming by such animals. However, these studies became saturated and gave rise to a whole group of animals that become neglected regarding their venoms and secretions. This repertoire of unexplored toxins and venoms bears biotechnological potential, including the development of new technologies, therapeutic agents and diagnostic tools and must, therefore, be assessed. In this review, we will approach such topics through an interconnected historical and scientific perspective that will bring up the major discoveries and innovations in toxinology, achieved by researchers from the Butantan Institute and others, and describe some of the major research outcomes from the study of these neglected animals.

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Venom gland transcriptome from Heloderma horridum horridum by high-throughput sequencing

Cited by 8 publications

References 97 publications

SMRT sequencing of the full-length transcriptome of Gekko gecko

SMRT sequencing of the full-length transcriptome of Gekko gecko

Proteomic Analysis of Heloderma horridum horridum Venom: Assessment to Its Transcriptome and Newfound Proteins

Neglected Venomous Animals and Toxins: Underrated Biotechnological Tools in Drug Development

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