Venous Antithrombotic and Anticoagulant Activities of a Fucoïdan Fraction

Mauray, Sandrine; Sternberg, C.; Theveniaux, Jocelyne; Millet, J.; Sinquin, Corinne; Tapon‐Bretaudière, Jacqueline; Fischer, Anne‐Marie

doi:10.1055/s-0038-1649927

Cited by 100 publications

(66 citation statements)

References 17 publications

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“…Algal fucans have anticoagulant/antithrombotic (5,8,9), anticomplement (10), antiproliferative (11), antiviral (12), and antiadhesive activities (13). However, the relationship between structure and biological activity of fucans has not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

Silva

Alves

Queiroz

et al. 2005

Braz J Med Biol Res

131

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The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-Dglucuronic acid 1→ or 4-ß-D-glucuronic acid 1 →, substituted at C-2 with α-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-α-L-fucose 1→ units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-Osulfation at C-3 of 4-α-L-fucose 1→ units is responsible for the anticoagulant activity of the polymer. Correspondence

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Section: Introductionmentioning

confidence: 99%

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

Silva

Alves

Queiroz

et al. 2005

Braz J Med Biol Res

131

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“…Mechanisms related to both antithrombin and heparin cofactor II-mediated activity have been described for algal-sulfated fucans from different species (2,4,6,23). Direct anticoagulant activity by an antithrombin-independent pathway has also been reported for some preparations of algal fucans (24).…”

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confidence: 99%

“…Algal-sulfated fucans have anticoagulant activity as measured in several different assays (2)(3)(4)(5) and have also venous antithrombotic activity (6). These polysaccharides are inhibitors of native (7) and recombinant (8) human immunodeficiency virus reverse transcriptase activity in vitro.…”

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confidence: 99%

Structure and Anticoagulant Activity of Sulfated Fucans

Pereira

Mulloy

Mourāo

1999

Journal of Biological Chemistry

349

132

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Sulfated fucans are among the most widely studied of all the sulfated polysaccharides of non-mammalian origin that exhibit biological activities in mammalian systems. Examples of these polysaccharides extracted from echinoderms have simple structures, composed of oligosaccharide repeating units within which the residues differ by specific patterns of sulfation among different species. In contrast the algal fucans may have some regular repeating structure but are clearly more heterogeneous when compared with the echinoderm fucans. The structures of the sulfated fucans from brown algae also vary from species to species. We compared the anticoagulant activity of the regular and repetitive fucans from echinoderms with that of the more heterogeneous fucans from three species of brown algae. Our results indicate that different structural features determine not only the anticoagulant potency of the sulfated fucans but also the mechanism by which they exert this activity. Thus, the branched fucans from brown algae are direct inhibitors of thrombin, whereas the linear fucans from echinoderms require the presence of antithrombin or heparin cofactor II for inhibition of thrombin, as reported for mammalian glycosaminoglycans. The linear sulfated fucans from echinoderms have an anticoagulant action resembling that of mammalian dermatan sulfate and a modest action through antithrombin. A single difference of one sulfate ester per tetrasaccharide repeating unit modifies the anticoagulant activity of the polysaccharide markedly. Possibly the spatial arrangements of sulfate esters in the repeating tetrasaccharide unit of the echinoderm fucan mimics the site in dermatan sulfate with high affinity for heparin cofactor II.Since the description of high amounts of sulfated fucans in marine brown algae over 50 years ago (1), these polysaccharides have been widely tested for biological activities in different mammalian systems. Algal-sulfated fucans have anticoagulant activity as measured in several different assays (2-5) and have also venous antithrombotic activity (6). These polysaccharides are inhibitors of native (7) and recombinant (8) human immunodeficiency virus reverse transcriptase activity in vitro.

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“…Fucoidans possess exceptional pharmaceutical activities such as anticoagulant [19], antibacterial and antiviral [20,21,22], antithrombotic [23], antitumoral [24], antiinflammatory [25], antiproliferative [17], and antifertilizing [18]. They are also successfully used for prevention and treatment of such widespread diseases as high blood pressure, arthritis, diabetes and obesity.…”

Section: Figmentioning

confidence: 99%

Studies on Fucoidan and Its Production From Vietnamese Brown Seaweeds

Lý¹,

Buu²,

Nhut³

et al. 2017

AJSTD

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Sulfated fucans are among the most widely studied of all the sulfated polysaccharides of plant origin that exhibit biological activities in mammalian systems. In this report fucoidans from some Vietnamese Sargassum species such as S. polycystum, S. oligocystum, S. mcclurei, S. swartzii and denticaprum were extracted and fractionated on a DEAE-Sephadex A-25 column.On the basis of chemical and spectral analyses, the fucoidan fractions obtained were found to be the sulfated fucogalactans containing sulfate ester groups and uronic acid, and composed essentially of fucose and galactose, as well as a minor amount of other sugars. The polysaccharide fractions were tested for anticancer activity. The primarily obtained results showed that all fucoidan fractions isolated from S. swartzii demonstrate bioactivity effects against cancer cells, while fraction F5 with a highest sulfate content exhibits the strongest antiinvasion activity. This indicates that sulfate content plays an important role in the anticancer activity of the brown algal fucoidans.A laboratory scale pilot for fucoidan production from Vietnamese brown seaweeds has been set with a capacity of 500 g of crude fucoidan per day.

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Venous Antithrombotic and Anticoagulant Activities of a Fucoïdan Fraction

Cited by 100 publications

References 17 publications

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

Structure and Anticoagulant Activity of Sulfated Fucans

Studies on Fucoidan and Its Production From Vietnamese Brown Seaweeds

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