Verapamil Increases the Nephrotoxic Potential of Gentamicin in Rats

Abstract

The effect of verapamil on the nephrotoxic potential of gentamicin was examined in rats. Rats were injected with one of the two or both drugs for 6 days and sacrificed 48 h after the last injection. In verapamil-treated rats (5 mg/kg/day), no histological or biochemical evidence of renal injury was detected. In all gentamicin-treated rats (100 mg/kg/day), a significant increase in kidney weight was observed (p < 0.001). The renal histopathologic lesions were more extensive in rats treated simultaneously with both gentamicin and verapamil. These rats exhibited a decline in body weight earlier (from day 4) than rats injected with gentamicin alone (from day 6). Serum urea nitrogen and creatinine in the latter group of rats were 24.9 ± 3.7 and 1.1 ± 0.1 versus 47.4 ± 8.6 and 1.6 ± 0.2 mg/dl, respectively, in rats treated with the combined therapy (p < 0.05). These results indicate that verapamil increases the severity of gentamicin nephrotoxicity. This interaction should be considered as a factor that can potentially increase the nephrotoxic risk associated with gentamicin administration.

Modulation of nephrotoxicity induced by gentamicin with bone marrow mesenchymal stem cells and Moringa oleifera extract

“…The aminoglycoside gentamicin (GN) is widely antibiotic used in the treatment of infection caused by gram negative bacteria [2]. It was defined to possess significant nephrotoxic action in man and experimental animals [3] due to its accumulation in proximal renal tubules which in turn leads to brush border network damage [4]. The nephrotoxicity involves renal free radical production and accumulation, which had been suggested as a causative agent of cell death in different pathological states including various models of renal diseases [5] such as consumption of antioxidant defense mechanisms, glomerular Egypt.J.Chem.…”