Verbascoside suppresses the migration and invasion of human glioblastoma cells via targeting c-Met-mediated epithelial-mesenchymal transition

Hei, Bo; Wang, Jia; Wu, Guangyong; Ouyang, Jia; Liu, Ruen

doi:10.1016/j.bbrc.2019.05.096

Cited by 16 publications

(14 citation statements)

References 20 publications

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“…Initial results in animal models are promising but need to be translated to clinical setting. Treatments utilising these compounds are likely to be well tolerated, as initial animal experimentation has demonstrated [43,50,65,85]. Nevertheless, initial investigation is encouraging in relation to the prevention and treatment of cancer.…”

Section: Resultsmentioning

confidence: 99%

“…Apoptosis promotion by verbascoside is associated with HIPK2, p53, HIF-1α and Rac-1 in colorectal cancer cell lines [83,84], in addition to downregulation of STAT3, epithelial-tomesenchymal transition (EMT) markers (vimentin, snail and zeb1) and c-Met in glioblastomas [85,86]. Hei et al [85] further demonstrated the downregulation of the EMT markers and c-Met in an orthotopic glioblastoma xenograft mouse model. EMT is a fundamental hallmark of metastatic tumourigenesis and has an essential role in glioma aggressiveness [87].…”

Section: Flavonoidsmentioning

confidence: 96%

“…EMT is a fundamental hallmark of metastatic tumourigenesis and has an essential role in glioma aggressiveness [87]. Hei et al [85] highlighted that verbascoside can bind directly to the c-Met protein, and that verbascoside causes c-Met protein degradation through the ubiquitination-proteasome pathway. Furthermore, verbascoside was able to suppress tumour growth and enhance survival in mice [85].…”

Section: Flavonoidsmentioning

confidence: 99%

“…Hei et al [85] highlighted that verbascoside can bind directly to the c-Met protein, and that verbascoside causes c-Met protein degradation through the ubiquitination-proteasome pathway. Furthermore, verbascoside was able to suppress tumour growth and enhance survival in mice [85]. This model demonstrated that verbascoside exerted effects via the same mechanisms in vivo as it did in vitro, by suppressing c-Met-mediated EMT and inducing cancer death.…”

Section: Flavonoidsmentioning

confidence: 99%

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The Anti-cancer Effect of Olea europaea L. Products: a Review

Antoniou

Hull

2021

Curr Nutr Rep

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Purpose of Review The olive tree (Olea europaea L.) has featured as a significant part of medicinal history, used to treat a variety of ailments within folk medicine. The Mediterranean diet, which is rich in olive products, is testament to Olea europaeas positive effects on health, associated with reduced incidences of cancer and cardiovascular disease. This review aims to summarise the current literature regarding the therapeutic potential of Olea europaea products in cancer, detailing the possible compounds responsible for its chemotherapeutic effects. Recent Findings Much of the existing research has focused on the use of cell culture models of disease, demonstrating Olea europaea extracts, and specific compounds within these extracts, have efficacy in a range of in vitro and in vivo cancer models. The source of Olea europaeas cytotoxicity is yet to be fully defined; however, compounds such as oleuropein and verbascoside have independent cytotoxic effects on animal models of cancer. Summary Initial results from animal models are promising but need to be translated to a clinical setting. Treatments utilising these compounds are likely to be well tolerated and represent a promising direction for future research.

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Section: Resultsmentioning

confidence: 99%

Section: Flavonoidsmentioning

confidence: 96%

Section: Flavonoidsmentioning

confidence: 99%

Section: Flavonoidsmentioning

confidence: 99%

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The Anti-cancer Effect of Olea europaea L. Products: a Review

Antoniou

Hull

2021

Curr Nutr Rep

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“…Previously, Hei et al has demonstrated that verbascoside suppressed the migration and invasion of human glioblastoma cells via targeting the c-Met-mediated EMT. 32 Here, we showed that verbascoside inhibited EMT through HMGB1/RAGE axis and the subsequent TGF-β/Smad signaling (Figures 5 and 6). Our data demonstrated that verbascoside may be a beneficial supplement to attenuate the progression of prostate cancer through inhibition of TGF-β-associated EMT by HMGB1/ RAGE axis.…”

Section: Discussionmentioning

confidence: 70%

Verbascoside inhibits the epithelial‐mesenchymal transition of prostate cancer cells through high‐mobility group box 1/receptor for advanced glycation end‐products/TGF‐β pathway

Chen

Hsieh

et al. 2021

Environmental Toxicology

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Introduction: Prostate cancer has significant mortality and metastasis rate in the male. Unfortunately, effective treatment for patients with advanced prostate cancer is still lacking. Verbascoside, a phenylethanoid glycoside, displays various pharmacological properties, such as the anti-cancer activities. The present study aimed to evaluate the effects of purified verbascoside on human prostate cancer and the associated molecular mechanisms. Materials and Methods:The human prostate cancer cell lines, Du-145 and PC-3, were treated with various concentrations of verbascoside (0.1, 1, 10 μM) for 24 h followed by the examination of cell viability using MTT and trypan blue exclusion assays. Cell migration and invasion capacities were assessed by wound healing assay and transwell system. Western blot and immunofluorescence staining were used to detect the expression of epithelial-mesenchymal transition (EMT)-associated factors, components of transforming growth factor (TGF-β)/Smad signaling, and high-mobility group box (HMGB)/receptor for advanced glycation end-products (RAGE) axis.Results: Verbascoside treatment significantly inhibited cell proliferation, migration, and invasion abilities of Du-145 and PC-3 cells. We showed that verbascoside decreased the expression of EMT promotors, Snail and Slug, and increased the expression of E-cadherin. Moreover, the expression level of alpha-smooth muscle actin was downregulated by verbascoside as well. Besides, we found that the TGF-β pathway was suppressed, which was demonstrated by the diminished expression of type I and II TGF-β receptors and phosphorylated Smad2/3 along with the upregulated Smad7. Our data suggested that this downregulation of TGF-β signaling was mediated by repression of HMGB 1 (HMGB1)/RAGE axis. Conclusion:Verbascoside mitigated the cell proliferation and aggressiveness of prostate cancer via downregulation of TGF-β-associated EMT progression through Chun-Hsien Wu and Chung-Hsien Chen contributed equally to this study.

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Verbascoside enhances radiosensitivity of hepatocellular carcinoma cells through regulating miR‐101‐3p/Wee1 axis

Sun

Cai

Zhou

et al. 2022

Drug Development Research

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Verbascoside is a kind of phenylpropanoid glycoside derived from multiple medicinal plants, exerting anti-tumor effects in diverse human malignancies. However, the function of Verbascoside on the radiosensitivity of hepatocellular carcinoma (HCC) cells remains unknown. Human Huh7 and HepG2 cell lines were treated with Verbascosideis, and cell viability was detected with cell counting kit-8 (CCK-8) assay. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect miR-101-3p expression, and Western blot was used to quantify the expression of WEE1 G2 checkpoint kinase (WEE1). Then, CCK-8 and flow cytometry assays were used to detect the proliferation and apoptosis of HCC cells after Verbascoside and X-ray combined treatment, and the expressions of WEE1 and apoptosis-related proteins Bax and Bcl-2 were detected by Western blot. Verbascoside could improve the radiosensitivity of HCC cells in a dosedependent manner. Verbascoside increased the expression of miR-101-3p but reduced WEE1 expression in HCC cells. Additionally, WEE1 was identified as a target of miR-101-3p. MiR-101-3p inhibition or WEE1 overexpression could reverse the effect of Verbascoside on the viability and apoptosis of HCC cells. Verbascoside increases the radiosensitivity of hepatocellular carcinoma cells via modulating miR-101-3p/WEE1 axis.

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Verbascoside suppresses the migration and invasion of human glioblastoma cells via targeting c-Met-mediated epithelial-mesenchymal transition

Cited by 16 publications

References 20 publications

The Anti-cancer Effect of Olea europaea L. Products: a Review

The Anti-cancer Effect of Olea europaea L. Products: a Review

Verbascoside inhibits the epithelial‐mesenchymal transition of prostate cancer cells through high‐mobility group box 1/receptor for advanced glycation end‐products/TGF‐β pathway

Verbascoside enhances radiosensitivity of hepatocellular carcinoma cells through regulating miR‐101‐3p/Wee1 axis

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