Vergleich von Methoden zur Aktivitätsbestimmung der Serumcholinesterasen (Acylcholin-acylhydrolase E. C. 3.1.1.8) und deren diagnostische Wertigkeit

Abstract

Zusammenfassung: Die Aktivitäten der Serumcholinesterasen wurden mit den Substraten Acetyl-, Butyryl-und Propionylthiocholinjodid parallel bei einem Normalkollektiv und Patienten mit akuter und chronischer Hepatitis, Lebercirrhose, Fettleber, Cholestase, Intoxikationen und malignen Tumoren bestimmt. Als Normbereiche ergaben sich mit den aufgeführten Substraten folgende Werte: Acetylthiocholinjodid:1,3 -3,7 kU/1 (Männer), 1,22-3,2 kU/1 (Frauen) Butyrylthiocholinjodid:2,27-7,40 kU/1 (Männer), 2,05-6,70 kU/1 (Frauen) Propionylthiocholinjodid: 3,10-7,5 kU/1 (Männer), 2,90-6,90 kU/1 (Frauen) Die Korrelation zwischen den verschiedenen Methoden, besonders zwischen Butyryl-und Propionylthiocholinjodid sind hoch signifikant. Gegenüber der Kontrollgruppe sind die Serumaktivitäten aller drei Methoden bei Patienten mit akuter und chronischer Hepatitis, Lebercirrhose, Intoxikationen und malignen Tumoren signifikant vermindert. Eine Veränderung der Spezifität der Serumcholinesterasen gegenüber Acetyl-, Butyryl-und Propionylthiocholinjodid bei Normalpersonen und Patienten mit schwerer akuter Hepatitis bzw. Lebercirrhose wurde nicht beobachtet. In allen Fällen kam es auch im Verlaufe eines exogenen oder endogenen Leberkomas zu einem parallelen Abfall der Serumenzymaktivitäten . Comparative methods for the determination of the activity of serumcholinesterases ( acylchotin-acyl-hydrofase E. C. 3.1. J. 8) and their diagnostical valueSummary: The activities of serum cholinesterases were determined in parallel with acetyl-, butyryl-and propionylthiocholiniodide in healthy persons and patients with acute and chronic hepatitis, cirrhosis of the liver, fatty liver, cholestasis, intoxication and malignant tumors.The following normal values were obtained: Acetylthiocholiniodide:1.3 -4.7 kU/1 (men), Butyrylthiocholiniodide:2.27-7.40 kU/1 (men), Propionylthiocholiniodide: 3.10-7.5 kU/1 (men),

Evaluation of the UV-340 Spectrophotometric Determination for Pseudocholinesterase Activity (EC 3.1.1.8) in Human Serum

“…The catalytic concentration was significantly lower in females than in males, äs determined by both methods (p < 0.0001). This sex difference was also observed by other investigators (4,9,14). The catalytic activity using the UV-340 method was significantly higher (p < 0.0001, Wilcoxon signed rank test) than that determined by the reference method (tab.…”

“…Several colorimetric methods for the determination of serum cholinesterase catalytic concentration have been develpped using iodide salts of butyiyl-, benzoyK acetyl· or propiöiiylchöline äs Substrates (4)(5)(6). Recently, Ashihara and coworkers developed a method with the new Substrate /j-hydroxybenzoylcholine and with p-hydroxybenzoate hydjoxylase äs a coupled ei&zyme (7).…”

Determination of acetylcholinesterase and butyrylcholinesterase activity without dilution of biological samples

“…BChE assay can be applied for e.g. acute hepatitis or liver cirrhosis diagnosis (Prellwitz et al, 1976;Kemkes-Matthes et al, 1987). Beside the *Corresponding author, e-mail: miroslav.pohanka@gmail.com Brought to you by | New York University Bobst Library Technical Services Authenticated Download Date | 10/9/15 5:04 PM diagnostic purposes, cholinesterases can be used in the construction of biosensors for neurotoxic compounds assay.…”

Comparison of Substrates for Measuring Serum Choline Esterase Activity in Hepato-Biliary Disease

“…With respect to the specificity of Substrates for measuring serum pseudocholine esterase for the ässessment of hepatic function, Prellwitz et al (14) have reported that in hepatic cirrhosis and also in chronic hepatitis the activities of serum choline esterase determined at 25 °C with acetyl-, butyryl-, or propionylthiocholine all showed similar decreases, and revealed no significant differences ja the specificities of substrates. Likewise, in the present study the decreased choline esterase activity in hepatic cirrhosis was observed with each of these Substrates.…”

“…On the other band, for assessing hepatic function no significant difference among acetyl-, butyryl-and propionyl-thiocholine was demonstrated by studying choline esterase activity at 25 °C (14). At present, no comparative study is available with respect to assay temperature for the evaluation of hepatic function.…”