Vergleichende Untersuchungen �ber den Gehalt verschiedener Tierhypophysen an Melanophoren- und Erythrophorenhormon

Jores, A.; Hotop, Hans

doi:10.1007/bf00339160

Cited by 7 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Koller and Rodewald [1933] claimed that extracts of pituitaries from frogs maintained in complete darkness have a lower melanophoreexpanding potency than similar extracts prepared from frogs maintained under constant illumination. Jores Jores and Hoeltz, 1936] confirmed this result using both saline and weak acid for extraction, but claimed that the difference between the two extracts was eliminated by the use of caustic soda extraction. He interpreted this in the following way.…”

Section: The Response Of Xenopus To Various B-containingmentioning

confidence: 83%

“…Activity. (a) claimed that there was a migration of pigment after the instillation of B-containing extracts into the human eye, and the vision of the treated eye was thought to show more rapid dark adaptation than the control [Jores and Hotop, 1934]. Their findings were not confirmed by Buschke [1934] using material supplied to him by Jores.…”

Section: Potential Effect8 Due To the Fact That B Control8 Melanophorementioning

confidence: 99%

See 1 more Smart Citation

Intermediate Lobe Pituitary Hormone

Landgrebe¹,

Waring²

1941

Exp Physiol

View full text Add to dashboard Cite

1. The optimum source for the manufacture of melanophore hormone (B hormone) is the posterior lobe of the pituitary, provided that the pressor and oxytocic autacoids can be removed without recourse to caustic soda treatment. 2. Previous methods of assaying melanophore activity are criticised, and the advantages of hypophysectomised Xenopus lævis as a test animal are emphasised. 3. A provisional unit of melanophore activity is proposed. 4. A new and rapid method is described for the preparation of B extracts which are substantially free from pressor substance (less than 1 unit of pressor activity in 4000 melanophore units) and oxytocin (less than 1 unit in 40,000 melanophore units). The final product in powder form keeps indefinitely and may be standardised on a weight basis with fair accuracy. 5. The preparation of B‐containing pituitary extracts is examined in detail. The oxytocic, pressor, and melanophore principles from posterior‐lobe extract are adsorbed by active charcoal. In 1 hour 25 p. c. of the melanophore activity can be eluted from the charcoal with pure water‐free liquid phenol. In this time little or no vasopressin and oxytocin are eluted. 6. The rôle of caustic soda, hitherto widely used to remove pressor and oxytocic properties from B‐containing extracts, is explored. Caustic soda treatment of general extracts modifies the melanophoric properties in at least two ways: (a) the melanophore‐expanding potency is increased; (b) there is an increased duration of the response when sub‐maximal doses are injected. 7. Differences in the response curves of hypophysectomisedXenopus to caustic treated and untreated whole pituitary extracts are not primarily due to the destruction of the pressor activity. In crude posterior‐lobe extracts there is a substance other than pressor autacoid or B‐precursor which, after treatment with caustic, modifies the melanophore response evoked by B hormone in the two ways noted. This substance is adsorbed on to charcoal from untreated extracts but is not eluted in 1 hour by phenol. After treatment of the general extract with caustic soda this substance, presumably modified by caustic, is adsorbed by charcoal, and is now eluted, together with melanophore‐dispersing hormone, by phenol. This substance may be oxytocin. 8. Since the alterations brought about by caustic treatment (para‐graph 6 above) in the melanophoric properties of an extract depend on the effect of the alkali on some pituitary constituent other than B, quantitative estimations of the B content of pituitary substance or of body fluids (e. g. blood) cannot furnish reliable data if caustic soda is used in the preparation of the extracts injected into the test animal. 9. Evidence is presented to substantiate the view that melanophore‐dispersing hormone is not passed by the kidney but is destroyed in the tissues. We were unable to detect the presence of B hormone in the urine of normal human beings. 10. Recent findings on the physiological role or pharmacodynamic effect of B hormone, other than the co‐ordination of chro...

show abstract

Section: The Response Of Xenopus To Various B-containingmentioning

confidence: 83%

Section: Potential Effect8 Due To the Fact That B Control8 Melanophorementioning

confidence: 99%

Intermediate Lobe Pituitary Hormone

Landgrebe¹,

Waring²

1941

Exp Physiol

View full text Add to dashboard Cite

show abstract

“…The same -may h'old'good in' man, for whe;n Jores (1936) injected the hormoine intraven6usly into normal subjec'ts he-found that it rapidly 'disappeared from't'he circulation. If this IS true, and if secretion from the pituitary is intermittent, " positive-" ';results might be obtained in a subject atb-one time and " negative" results at another.…”

Section: Melanophore-expanding Substance In Urine Ementioning

confidence: 89%

“…Thus when Jores and Caesar (1935) treated a' frog's eye with a soltition of B-hormone the retinal,pigment migrated to -the dark position more rapidly. After instilling extracts containing B-hormone into the human eye, Jores (1933) Jores and Hotop-(1934) and Bastile (1939) found that -dark adaptation occurred more rapidly in the treated than in the untreated eye, and also more rapidly than in a normal eye.…”

Section: The Roie Of B-hormone In Visionmentioning

confidence: 99%

“…-In this particular case no 441 JOSEPH R. MUTCH-AND DONALD MACKAY reason -(e.g., ingestion of drugs, intercurrent disease) could be found to explain the positive result. Jores (1936) found that treatment with. charcoal did not invariably abolish the urinary reaction which he therefore did not -consider specific for B-hormone.…”

Section: Melanophore-expanding Substance In Urine Ementioning

confidence: 99%

See 1 more Smart Citation