Verification of WFA-Sialylated MUC1 as a Sensitive Biliary Biomarker for Human Biliary Tract Cancer

Abstract

Biliary WFA-sialylated MUC1 is a useful biomarker for the differentiation of biliary tract cancer. The sensitivity of WFA-sialylated MUC1 was clearly higher than that of biliary cytology. Further data collection is necessary to validate the clinical usefulness of this biomarker.

Wisteria floribunda Agglutinin and Its Reactive-Glycan-Carrying Prostate-Specific Antigen as a Novel Diagnostic and Prognostic Marker of Prostate Cancer

“…They stated that LacdiNAc in N -glycans significantly decreases during progression of human breast cancer [13,14]. In contrast, the enhanced expression of LacdiNAc has been shown to be associated with the progression of human prostate, ovarian, colon, and liver cancers [12,15,16,17]. Therefore, the quantification of LacdiNAc glycan carrying glycoproteins or tissue-specific expression of LacdiNAc glycan detected by the WFA has shown promise as cancer glycobiomarkers [17,18,19].…”

“…In contrast, the enhanced expression of LacdiNAc has been shown to be associated with the progression of human prostate, ovarian, colon, and liver cancers [12,15,16,17]. Therefore, the quantification of LacdiNAc glycan carrying glycoproteins or tissue-specific expression of LacdiNAc glycan detected by the WFA has shown promise as cancer glycobiomarkers [17,18,19]. In particular, regarding PCa, there are only three papers about LacdiNAc distribution in prostate biopsy (Pbx) and RP specimens using WFA [15,16,20], and they did not report the relation between WFA-reactivity in tissues and PCa prognosis.…”

Molecular Basis for Recognition of the Cancer Glycobiomarker, LacdiNAc (GalNAc[β1→4]GlcNAc), by Wisteria floribunda Agglutinin

“…Thus, its cross-reactive potential severely limits its use for cancer biomarker detection. WFA is currently considered the most prominent diagnostic lectin against cholangiocarcinoma when compared with other homologous lectins (26,35) and the most discriminatory against altered N-glycans on Mac2-binding protein, a secretory N-glycoprotein with elevated expression levels during viral hepatitis induced liver cirrhosis (36,37).…”

Polyporus squamosus Lectin 1a (PSL1a) Exhibits Cytotoxicity in Mammalian Cells by Disruption of Focal Adhesions, Inhibition of Protein Synthesis and Induction of Apoptosis

“…In many cancer cells, malignancy is correlated with overexpression of sialic acids on the cell surface [22]. It has further been shown that secreted sialylated mucins can be detected in the bloodstream of cancer patients [23], and lectins are valuable diagnostic tools [24–26]. Thus, sialic acid specific lectins are useful tools for the detection and characterization of sialylated glycoconjugates displayed on the surface of cancer cells and tissues [27, 28].…”