Verifikasi molekuler metode sexing sperma sapi dengan kolom BSA (Bovine Serum Albumin)

Kaiin, Ekayanti Mulyawati; Gunawan, Muhammad; Octaviana, Senlie; Nuswantara, Sukma

doi:10.13057/psnmbi/m030214

Cited by 5 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…If the motility is <70%, it can still be used if the recovery rate is at least 50% (BSN, 2017). Production of frozen sexed semen using 5% and 10% BSA columns can only be performed if the motility percentage value is 60% to anticipate a drastic decrease in sperm quality due to the incubation treatment for 40–60 min longer than the usual freezing process [ 8 ]. In addition, the sperm was 1750 ± 100 × 10 6 cells/mL.…”

Section: Resultsmentioning

confidence: 99%

“…This method does not damage the acrosomal integrity of sperm or sexed sperm yield, which is one of the reasons why it is preferred. Bovine serum albumin column methods have a conception rate (CR) similar to that of conventional semen of more than 85% [ 8 ] or use egg white albumin [ 9 ]. This technique is expected to prevent a decline in the quality of spermatozoa after the sexing process, because the BSA gradient method does not excessively manipulate spermatozoa [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols

et al. 2023

View full text Add to dashboard Cite

Background and Aim: To enhance the reproductive potential and increase productivity and population of cows, spermatozoa sex-sorting technology is required. This study aimed to examine the effect of sexing sperm, separated using a bovine serum albumin (BSA) column with varying incubation durations and centrifugation methods, for successful artificial insemination. Materials and Methods: Six Simmental bulls and 30 cows (n = 30) as the recipients were selected for this study at Balai Pembibitaan Hijauan Pakan Ternak Sembawa Indonesia. The study parameters included sperm motility, viability, plasma membrane integrity, and conception rate (CR). The experiment was divided into three protocols to find out differences in some parameters: (1) BSA incubation time effect (P) with P1 (40 min), P2 (50 min), and P3 (60 min); (2) freezing time effect with before freezing and after-thawing treatments; and (3) CR determined by measuring the proportion of pregnant cows following insemination with non-sexed, X-bearing, and Y-bearing sperms without centrifugation (n = 15) (A0, A1, and A2) and with centrifugation (n = 15) (B0, B1, and B2) in the acquired data, which were counted using the Statistical Package for the Social Sciences version 21 program. Analysis of variance was utilized to evaluate all treatments at various levels. Results: The results demonstrated that centrifugation time influenced all sperm quality metrics for sperm containing X and Y (p < 0.05). The non-return rate (NRR) of non-sexed frozen semen, both centrifuged (A0) and not centrifuged (B0), was more significant than frozen semen produced by sexing X and Y spermatozoa. The NRR indicated a value of 80% based on the number of lactating cows. Conclusion: Bovine serum albumin incubation and centrifugation protocols influenced and decreased all sperm quality indicators throughout the sexing procedure and could still be used as a sexing protocol. Furthermore, regarding NRR and service per conception, non-sexual treatment is superior to sexing treatment. Keywords: bovine serum albumin, centrifugated, conception rate, incubation, sexing, sperm.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The frozen semen is processed for sexing spermatozoa using a 5-10% BSA gradient column for 45 min, as described by Kaiin and Gunawan (2017). Following the sexing process, the spermatozoa collected from each gradient of BSA column were then centrifuged in Brackett Oliphant (BO) medium for 10 min at 1800 rpm.…”

Section: Sperm Separation Using Bovine Serum Albumin (Bsa) Methodsmentioning

confidence: 99%

“…It has resulted in an 81% sex compatibility rate for dairy calves with a sex chromosome ratio (S/C) of 1.37 (Said et al, 2005) and the production of embryos through IVF, leading to the development of embryos to the morula and blastocyst stages (Kaiin et al, 2008). Notably, Gunawan et al (2015) achieved a sexing success rate of 87% for spermatozoa X and 89.5% for spermatozoa Y, while in Simmental cattle, the rate for spermatozoa Y reached 81.7% (Kaiin et al, 2008). In Bali cattle, a rate of 76.7% was observed for spermatozoa X (Gunawan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The Quality of Frozen Buffalo Sperm Following Sexing using Bovine Serum Albumin (BSA) Column and Swim-Up (SU) Methods

Masrizal,

Afriani,

Wahyudi

et al. 2024

BuletinPeternak

View full text Add to dashboard Cite

This study assesses the impact of two sperm sexing techniques, the Bovine Serum Albumin (BSA) column method, and the swim-up (SU) method, on frozen buffalo spermatozoa quality. A total of 50 straws of frozen buffalo semen were used in this study. Spermatozoa quality was evaluated before (post thawing) and after the spermatozoa sexing process. Spermatozoa trapped in BSA upper fraction, BSA lower fraction, SU upper fraction, and SU lower fraction were separately evaluated. The parameters measured consisted of spermatozoa motility, viability, intact plasma membrane, intact acrosome cap, and spermatozoa DNA integrity. The results indicated that the quality of post-thawing buffalo spermatozoa remained relatively high, with motility at 41%, viability at 64.48%, intact plasma membrane at 55.42%, intact acrosome cap at 47.12%, and sperm DNA integrity at 74.94%. However, the use of the BSA column method significantly (p<0.05) decreased spermatozoa quality in both the upper and lower fractions, resulting in motility levels of 34% and 32%, viability rates of 49.36% and 44.71%, intact plasma membrane percentages of 44.78% and 37.13%, intact acrosome cap figures of 37.58% and 33.27%, and sperm DNA integrity levels of 74.76% and 72.45%, respectively. In contrast, the application of the SU method proved effective in preserving post-thawing spermatozoa quality, yielding motility rates of 42% and 41%, viability levels of 63.62% and 62.78%, intact plasma membrane percentages of 54.42% and 54.74%, intact acrosome cap figures of 46.94% and 45.74%, and sperm DNA integrity values of 70.57% and 70.01%, respectively. In summary, after freezing, the SU method excel the BSA column method in maintaining the quality of buffalo spermatozoa post-thawing.

show abstract

“…Waktu inkubasi 45 menit adalah waktu optimum menghasilkan proporsi spermatozoa kromosom X-Y paling tinggi dibandingkan lama inkubasi 60 dan 75 menit dan kualitas semen terbaik pasca sexing, yaitu proporsi spermatozoa X sebesar 75,40%±3,20% dengan angka motilitas 75,89%±2,13% hasil ini diperoleh dari penelitian yang membandingkan lama waktu ingkubasi (45, 60 dan 75 menit) pada proses sexing spermatozoa kambing Peranakan Etawah menggunakan media sexing BSA (Solihati et al, 2017). Angka persentase motilitas 56,6% spermatozoa X, 57,3% spermatozoa Y sapi yang di sexing menggunakan medium BSA dengan lama inkubasi 45 menit (Kaiin et al, 2017). Persentase motilitas spermatozoa sapi hasil separasi kolom albumin dengan waktu inkubasi 60 menit diperoleh hasil yaitu 75% spermatozoa Y pada fraksi bawah, namun proporsi spermatozoa Y sangat rendah pada fraksi bawah yaitu 58,82 % (Afiati, 2004).…”

Section: Pendahuluanunclassified

Pengaruh medium dan lama inkubasi dalam proses sexing sperma terhadap kualitas semen kambing Boer

Anwar¹

2019

JIT

View full text Add to dashboard Cite

Penelitian ini bertujuan untuk mengetahui pengaruh medium dan waktu inkubasi pada sexing semen terhadap kualitas spermatozoa kambing Boer. Proses sexing menggunakan metode perbedaan densitas dengan menggunakan medium bovine serum albumin (BSA) dan SpermGrad (SG). Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan yaitu, BSA 40 menit (P1), BSA 50 menit (P2), BSA 60 menit (P3), SG 40 Menit (P4), SG 50 (P5) dan SG 60 menit (P6). Variabel yang diamati adalah kualitas spermatozoa (motilitas dan abnormalitas). Data dianalisis menggunakan analisis ragam diikuti uji lanjut berganda Duncan. Materi yang digunakan adalah semen kambing Boer. Hasil penelitian menunjukkan motilitas tertinggi terdapat pada fraksi atas (sperma X) dan fraksi bawah (sperma Y) terdapat pada perlakuan P1 (72,0 ±2,7% dan 72,0±2,7%) dan motilitas terendah pada perlakuan P6 (57,0±5,7% dan 58,0±5,7%). Persentase viabilitas sperma tertinggi fraksi atas terdapat pada P2 dan fraksi bawah P3 (74,4±1,9% dan 79,5±2,8%), yang terendah fraksi atas P1 dan fraksi bawah P6 (72,4±3,6% dan 74,1±3,2%). Selain itu, persentase abnormalitas sperma tertinggi pada fraksi atas pada perlakuan P1 dan fraksi bawah pada P6 (8,5±0,9% dan 5,4±1,2%). Hasil penelitian dapat disimpulkan bahwa tidak terdapat perbedaan yang nyata motilitas antara kedua medium, dan waktu inkubasi 50 menit optimum untuk mempertahankan kualitas semen.

show abstract

Verifikasi molekuler metode sexing sperma sapi dengan kolom BSA (Bovine Serum Albumin)

Cited by 5 publications

References 9 publications

The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols

The reproductive success of Simmental bovine after sex-sorting under various incubation and centrifugation protocols

The Quality of Frozen Buffalo Sperm Following Sexing using Bovine Serum Albumin (BSA) Column and Swim-Up (SU) Methods

Pengaruh medium dan lama inkubasi dalam proses sexing sperma terhadap kualitas semen kambing Boer

Contact Info

Product

Resources

About