Verne Grant and evolutionary studies of <i>Aquilegia</i>

Hodges, Scott A.; Fulton, Michelle; Yang, Jinshui; Whittall, Justen B.

doi:10.1046/j.1469-8137.2003.00950.x

Cited by 89 publications

(87 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Numerous historical genetic studies on species of Aquilegia suggest that some characters separating species are controlled, at least in part, by genes of large effect, while other characters are controlled by many genes of small effect (Hodges et al, 2003). However, with the exception of inheritance studies of flower orientation, flower color and spur length (Hodges et al, 2002), no data are available that show a direct association between quantitative traits and molecular markers.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Zhu¹,

Gao²,

Xu³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Species of the genus Aquilegia are exceptionally diverse in their floral morphology and color, commonly known as columbine. They are widely planted ornamentals and are highly attractive for hummingbirds. However, little is known about their genetic diversity. We examined the genetic diversity of the species and cultivars using amplified fragment length polymorphism (AFLP) markers. Sixteen EcoRI/MseI AFLP primer combinations produced 327 informative polymorphic bands, with a mean of 20.4 bands scored per primer. Jaccard's coefficient of similarity varied from 0.61 to 0.93, indicative of high levels of genetic variation. Cluster analysis using the unweighted pair group method with arithmetic mean algorithm placed the 64 accessions into two main clusters, each divided into two sub-clusters. The AFLP variability was significantly associated with the geographic origins, as the Asian species and the North American species grouped into two distinct clusters. The genetic diversity found among Aquilegia demonstrated the potential value of Chinese germplasm for cultivar improvement and for widening the genetic basis of breeding programs and breeding material selection. We concluded that AFLPs are informative and can provide significant insights for genetic diversity research in columbine species.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Zhu¹,

Gao²,

Xu³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The relationship between plants and pollinators -including co-evolution, pollinator sensory bias and associative learning (Schiestl and Johnson, 2013) -is particularly important for closely related floral species whose reproductive isolation is mediated by differential pollinator preference (Fulton and Hodges, 1999;Schemske and Bradshaw, 1999;Ramsey et al, 2003;Hodges et al, 2004;Aldridge and Campbell, 2007;Klahre et al, 2011). In many such cases the composition and class of volatiles in the scents overlap (Jürgens, 2004;Svensson et al, 2006;Waelti et al, 2008;Steiner et al, 2011).…”

Section: Research Articlementioning

confidence: 99%

Three floral volatiles contribute to differential pollinator attraction in monkeyflowers (Mimulus)

Byers

Bradshaw

Riffell

2013

Journal of Experimental Biology

121

118

View full text Add to dashboard Cite

Flowering plants employ a wide variety of signals, including scent, to attract the attention of pollinators. In this study we investigated the role of floral scent in mediating differential attraction between two species of monkeyflowers (Mimulus) reproductively isolated by pollinator preference. The emission rate and chemical identity of floral volatiles differ between the bumblebee-pollinated Mimulus lewisii and the hummingbird-pollinated M. cardinalis. Mimulus lewisii flowers produce an array of volatiles dominated by D-limonene, β-myrcene and E-β-ocimene. Of these three monoterpenes, M. cardinalis flowers produce only D-limonene, released at just 0.9% the rate of M. lewisii flowers. Using the Bombus vosnesenskii bumblebee, an important pollinator of M. lewisii, we conducted simultaneous gas chromatography with extracellular recordings in the bumblebee antennal lobe. Results from these experiments revealed that these three monoterpenes evoke significant neural responses, and that a synthetic mixture of the three volatiles evokes the same responses as the natural scent. Furthermore, the neural population shows enhanced responses to the M. lewisii scent over the scent of M. cardinalis. This neural response is reflected in behavior; in two-choice assays, bumblebees investigate artificial flowers scented with M. lewisii more frequently than ones scented with M. cardinalis, and in synthetic mixtures the three monoterpenes are necessary and sufficient to recapitulate responses to the natural scent of M. lewisii. In this system, floral scent alone is sufficient to elicit differential visitation by bumblebees, implying a strong role of scent in the maintenance of reproductive isolation between M. lewisii and M. cardinalis.

show abstract

“…In Aquilegia, punctuated evolution of longer spur lengths correlates with shifts to pollinators possessing longer tongues [5,6]. Early hypotheses suggested that these spurs are produced by the activity of 'meristematic knobs' flanking the petal attachment point [7,8].…”

Section: Introductionmentioning

confidence: 99%

Molecular basis for three-dimensional elaboration of theAquilegiapetal spur

et al. 2015

View full text Add to dashboard Cite

By enforcing specific pollinator interactions, Aquilegia petal nectar spurs maintain reproductive isolation between species. Spur development is the result of three-dimensional elaboration from a comparatively two-dimensional primordium. Initiated by localized, oriented cell divisions surrounding the incipient nectary, this process creates a pouch that is extended by anisotropic cell elongation. We hypothesized that the development of this evolutionary novelty could be promoted by non-mutually exclusive factors, including (i) prolonged, KNOX-dependent cell fate indeterminacy, (ii) localized organ sculpting and/or (iii) redeployment of hormone-signalling modules. Using cell division markers to guide transcriptome analysis of microdissected spur tissue, we present candidate mechanisms underlying spur outgrowth. We see dynamic expression of factors controlling cell proliferation and hormone signalling, but no evidence of contribution from indeterminacy factors. Transcriptome dynamics point to a novel recruitment event in which auxin-related factors that normally function at the organ margin were co-opted to this central structure. Functional perturbation of the transition between cell division and expansion reveals an unexpected asymmetric component of spur development. These findings indicate that the production of this three-dimensional form is an example of organ sculpting via localized cell division with novel contributions from hormone signalling, rather than a product of prolonged indeterminacy.

show abstract

Verne Grant and evolutionary studies of Aquilegia

Cited by 89 publications

References 51 publications

Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Three floral volatiles contribute to differential pollinator attraction in monkeyflowers (Mimulus)

Molecular basis for three-dimensional elaboration of theAquilegiapetal spur

Contact Info

Product

Resources

About