Vero cytotoxin-producing strains of Escherichia coli

Smith, H. R.; Scotland, Sylvia M.

doi:10.1099/00222615-26-2-77

Cited by 91 publications

(68 citation statements)

References 35 publications

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“…For example, Salmonella infections have gone up 20-fold in some countries in Europe since 1980 (3). Similar increases are reported for haemorhagic E. coli 0157 food poisoning: between 1986 and 1996, the frequency of infection increased 10-fold in England and Wales, and 100-fold in Scotland (4).…”

Section: Ha6e Infectious Diseases and Drug Resistance Gone Up Recently?supporting

confidence: 75%

“…In September the same year, the US Environmental Protection Agency (EPA) has authorized a biotech company to sell 500000 lb of a genetically engineered Rhizobium melitoti strain, for coating alfalfa seeds before planting. The GE Rhizobium 'contains an extra copy of the gene controlling nitrogenase production, genes to increase internal energy production, a gene conferring resistance to the antibiotics streptomycin and spectinomycin, and some uncharacterized DNA 4 '. The antibiotics are currently used in treating TB, the top killer in the world to-day.…”

Section: Safety Regulations Are Grossly Inadequate and Further Relaxmentioning

confidence: 99%

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Gene Technology and Gene Ecology of Infectious Diseases

1998

Microbial Ecology in Health and Disease

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Section: Ha6e Infectious Diseases and Drug Resistance Gone Up Recently?supporting

confidence: 75%

Section: Safety Regulations Are Grossly Inadequate and Further Relaxmentioning

confidence: 99%

Gene Technology and Gene Ecology of Infectious Diseases

1998

Microbial Ecology in Health and Disease

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“…Of V'TEC 0 serogroups previously found in Sri Lanka [31], 0 86, 0 128, 0 84, 0 103, 0 111, and 0 153 have been associated with human diseases [7,. However, the H types of the strains isolated from Sri Lankan cattle were different from those associated with human disease in the temperate world [41]. There is little information on the role of VTEC in human disease in Sri Lanka.…”

Section: Discussionmentioning

confidence: 98%

A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka

Tokhi

Peiris

Scotland³

et al. 1993

Epidemiol. Infect.

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SUMIMIMARYTwo cohorts of 10 and 16 calves were followed at weekly or fortnightly intervals from 4-28 and 1-9 weeks respectively to determine whether natural infection by and an untvpable strain (0 ?.H21) were identified. All strains belonging to the samne serotype had identical profiles of reactivity with DNA probes to toxins VT1 or 2, LTI or II and a probe (CVD419) derived from a plasmid carried by enterohaemorrhagic Escherichia coli 0 157 . H7. Four of these serotypes were found in the faecal flora of the calves, taken as a group, throughout the 4-month study period.Sixty-percent of the strains hybridized with the probe for VT1, 4 % with the probe for V'T2, and 360% with both probes. Faecal V'TEC were significantly associated with overt diarrhoeal illness in animals < 10 weeks of age. but no characteristic profile of markers (serotype or hybridization pattern) in E. coli isolates was associated with diarrhoea. A serological response to VT1 wAas detected in some animnals, but faecal V-T1 V'TEC exeretion persisted in spite of seroconversion. VT1 seroconversion wNas not associated with diarrhoea. A serological response to NVT2 wras not detected even in those animals excreting VrT2 V'TEC in the faeces.

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“…Cytotoxicity assays were done essentially as described by Smith and Scotland [31]. Vero and Y-1 adrenal cells were grown in 96-well tissue culture plates (Costar, Cambridge, MA, USA) respectively in Eagle's Minimum Essential Medium (MEM; Sigma) supplemented with fetal calf serum (FCS) 5% v/v and Ham F-10 medium (Sigma) supplemented with L-glutamine 0.5% w/v and FCS 10% v/v.…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

“…The supernates were filtered through 0.45-,urn membrane filters (Millipore, Bedford, MA,USA) and stored at 4°C. Sterile culture supernates were then tested in the Y-1 cell assay for LT [32] and in the Vero cell assay for VT [31].…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

Enteropathogenicity markers in Escherichia coli isolated from infants with acute diarrhoea and healthy controls in Rio de Janeiro, Brazil

Rosa

Mariano

Pereira

et al. 1998

Journal of Medical Microbiology

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BACTERIAL PATHOGENICITY AND CHARACTERISATIONEn t ero pat h og en i ci ty m a r kers i n Escrt erichia coli isolated from infants with acute diarrhoea and healthy controls in Rio de Janeiro, Brazil Sa'o Paulo, Brazil Faeces from urban children < 2 years old with acute diarrhoeal illness and from nondiarrhoeal infants (controls) were examined for Escherichia coli and other enteropathogens. A total of 990 E. coli isolates from 100 patients and 50 controls was tested for enteropathogenic E. coli (EPEC) serotype (O:H), adherence to HEp-2 cells after incubation for 3 and 6 h, fluorescent actin staining (FAS), DNA hybridisation with EAF, eaeA, STh, STp and EAggEC probes and production of heat-labile enterotoxin (LT) and verocytotoxin (VT) with Y1 and Vero cells. EPEC were the most prevalent enteropathogens in patients (32.7%; and 14% in controls). Enteroinvasive E. coli (EIEC) and Vero cytotoxin-producing E. coli (VTEC) were not detected. The rate of isolation of enterotoxigenic E. coli (ETEC) was identical in both groups. Among the EPEC isolates the prevalent serotypes were Olll:H2, 055:NM and 01 19:H6. Localised adherence (LA) was found significantly more frequently in isolates from patients (19.6%) than controls (2.1%). All LA-positive EPEC isolates were FASf and eaeAf, but only 75.2% of them hybridised with the EAF probe. Diffusely adhering E. coli (DAEC) and enteroaggregative E. coli (EAggEC) were found with equal frequency in patients and controls. Twenty-seven E. coli isolates were negative for EAF but positive for eaeA and FAS and produced LA in 6-h adherence tests. These EAF-/eaeA+ strains were the only putative enteropathogen identified in seven patients and were not found in controls. The ability of these strains to elicit ultrastructural cell alterations and cell-signalling events was evaluated in Caco-2 cells (human colon carcinoma cell line) by the gentamicin invasion assay and by transmission electron microscopy. The numbers of intracellular bacteria in cell invasion tests varied from 0.4% to 1.6% of the cellassociated bacteria after a 6-h incubation period. Tyrosine phosphorylation of host cell proteins was assessed in HEp-2 cells by immunofluorescence microscopy and all strains gave positive results. EAF-/eaeA+ E. coli strains express most of the virulence properties found among true EPEC strains and can be a relevant cause of infant diarrhoea in developing countries.

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Vero cytotoxin-producing strains of Escherichia coli

Cited by 91 publications

References 35 publications

Gene Technology and Gene Ecology of Infectious Diseases

Gene Technology and Gene Ecology of Infectious Diseases

A longitudinal study of Vero cytotoxin producing Escherichia coli in cattle calves in Sri Lanka

Enteropathogenicity markers in Escherichia coli isolated from infants with acute diarrhoea and healthy controls in Rio de Janeiro, Brazil

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