Verocytotoxin-Producing Escherichia Coli 02:h5 Isolated From Patients With Ulcerative Colitis

Wulffen, H. von; Rüssmann, Holger; Karch, Helge; Meyer, Thomas; Bitzan, Martin; Kohrt, T.C.; Aleksic, S.

doi:10.1016/s0140-6736(89)90151-7

Cited by 39 publications

(23 citation statements)

References 6 publications

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“…This fluorescence in situ hybridization-based study allowed detection of the presence of bacteria independently of their transcriptional activity, unlike the rRNA-based TTGE approach chosen in the present work. This could thus suggest that (19), and several Enterobacteriaceae (17,28). However, these studies did not take bacterial metabolic activity into account.…”

Section: Discussionmentioning

confidence: 99%

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Temperature Gradient Gel Electrophoresis of Fecal 16S rRNA Reveals Active Escherichia coli in the Microbiota of Patients with Ulcerative Colitis

Sokol¹,

Lepage²,

Seksik

et al. 2006

J Clin Microbiol

134

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Previous studies of the endogenous microbiota in patients with ulcerative colitis (UC) have not taken bacterial activity into account, yet bacteria with high transcriptional activity might have a more important pathophysiological role than inactive bacteria. We therefore analyzed the biodiversity of active bacteria in the fecal microbiota of UC patients, in comparison with that of healthy subjects. Feces were collected from nine patients with active UC and from nine healthy controls. Total DNA and RNA were extracted, and 16S ribosomal DNA and RNA were amplified by PCR and reverse transcription-PCR, respectively. Amplification products were compared by means of temporal temperature gradient gel electrophoresis (TTGE). Bands of interest were excised, sequenced, and identified by comparison with the GenBank database (NCBI). The dominant-species diversity based on RNA-derived TTGE profiles was significantly lower for UC patients than for healthy controls (P ‫؍‬ 0.01). The mean similarity index between the "present" and "active" microbiota was 74% ؎ 18% for UC patients. Comparison of the individual "active" microbiota identified a band that was present for eight UC patients and only two controls (89% versus 22%; P ‫؍‬ 0.008). The band was sequenced for 6 patients and always corresponded to Escherichia coli. The biodiversity of active bacteria in the dominant fecal microbiota of patients with UC is lower than that of healthy subjects. E. coli is more represented in the active microbiota of UC patients. The possible pathophysiological role of this difference remains to be determined.

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Section: Discussionmentioning

confidence: 99%

“…In particular, bacteria appear to be more abundant in the UC colonic mucosa (26). Escherichia coli strains with unusual adhesive or pathogenic properties have also been detected in some patients (28).…”

mentioning

confidence: 99%

Temperature Gradient Gel Electrophoresis of Fecal 16S rRNA Reveals Active Escherichia coli in the Microbiota of Patients with Ulcerative Colitis

Sokol¹,

Lepage²,

Seksik

et al. 2006

J Clin Microbiol

134

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“…Adhesive Escherichia coli has been implicated in the pathogenesis of UC (16). In two separate studies, verotoxinproducing E. coli was isolated from the stools and rectal biopsies of patients during UC relapse or remission (17,18). In another study, however, stools from 34 patients diagnosed with active UC were negative for the bacteria (19).…”

Section: Bacterial Agents In Ulcerative Colitismentioning

confidence: 99%

The Role of Bacterial Infection in the Pathogenesis of Inflammatory Bowel Disease

2004

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“…The adhesive properties were similar to those of pathogenic intestinal E coli, suggesting that virulent E coli strains might participate in the pathogenesis of ulcerative colitis [43,44]. Another study reported adherence of only E. coli subtypes to rectal mucosa, however, no differences in adhesion could be found between ulcerative colitis patients and controls [45,46]. Using a hybridisation in situ technique, a significantly higher number of bacteria was found within the mucus layer and not adherent to the surface of the epithelium in ulcerative colitis patients compared with controls, independently from the degree of inflammation.…”

Section: Commentmentioning

confidence: 82%

Etiological Role of Sulfate-Reducing Bacteria in the Development of Inflammatory Bowel Diseases and Ulcerative Colitis

Kushkevych¹

2014

AJIDM

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Inflammatory bowel disease and ulcerative colitis are complex multifactorial diseases of unknown etiology. The etiological role of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 in the development of these inflammatory diseases in rats was studied. Applying sulfate-reducing bacteria, ulcerative colitis in the animals was experimentally induced at the fist time. In total 45 animals in three groups were separated and used in this experiment. Animals in the first group received the standard diet containing a special certified feed for rats and were used as a control. The second group received the standard feed and a dose (1 ml per each day) of modified Kravtsov-Sorokin's liquid medium for the initiation of own potential animal intestinal microflora of sulfate-reducing bacteria. The third group of the animals received the standard feed and dose of a suspension with D. piger Vib-7 and Desulfomicrobium sp. Rod-9 (at a ratio of 1 to 1) in the medium. The changes in the colonic microbiota under these conditions were studied. The bacteria belonging to the normal colonic microbiota were associated with the etiology of inflammatory bowel disease and ulcerative colitis. The concentration of sulfide and acetate in feces from different sections of the large intestine of the rats was determined. The level of ulcerations in the second and third groups of sick animals under the specific conditions was demonstrated. The described experimental results could be particularly useful for the study of inflammatory bowel disease and ulcerative colitis and its therapeutic strategy. These data are also indispensable to apply into mechanistic details that will facilitate better preclinical drug/therapy design to target specific components involved in the disease pathogenesis. Keywords

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Verocytotoxin-Producing Escherichia Coli 02:h5 Isolated From Patients With Ulcerative Colitis

Cited by 39 publications

References 6 publications

Temperature Gradient Gel Electrophoresis of Fecal 16S rRNA Reveals Active Escherichia coli in the Microbiota of Patients with Ulcerative Colitis

Temperature Gradient Gel Electrophoresis of Fecal 16S rRNA Reveals Active Escherichia coli in the Microbiota of Patients with Ulcerative Colitis

The Role of Bacterial Infection in the Pathogenesis of Inflammatory Bowel Disease

Etiological Role of Sulfate-Reducing Bacteria in the Development of Inflammatory Bowel Diseases and Ulcerative Colitis

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