Versatile Functionalization of Nanoelectrodes by Oligonucleotides via Pyrrole Electrochemistry

Abstract

Surface modification at the nanometer scale is a challenge for the future of molecular electronics. In particular, the precise anchoring and electrical addressing of biological scaffolds such as complex DNA nanonetworks is of importance for generating bio-directed assemblies of nano-objects for nanocircuit purposes. Herein, we consider the individual modification of nanoelectrodes with different oligonucleotide sequences by an electrochemically driven co-polymerization process of pyrrole and modified oligonucleotide sequences bearing pyrrole monomers. We demonstrate that this one-step technique presents the advantages of simplicity, localization of surface modification, mechanical, biological and chemical stability of the coatings, and high lateral resolution.

Functionalization of optical nanotip arrays with an electrochemical microcantilever for multiplexed DNA detection

“…The ODN-pyrrole immobilization was performed according to a procedure developed by Livache and coworkers. 48,52 The pyrrole-ODN used in this work presents the sequences:…”

A Review of Patterned Organic Bioelectronic Materials and their Biomedical Applications

“…The inherent characteristics of the materials are well embedded into the sensor designs for various schemes, including chemical conjugation of biorecognition molecules, enhanced sensitivity and specificity, low‐cost, portability, and disposability. As for the detection of DNA hybridization, CPs seem to offer more benefits over CNTs due to their capability to directly conjugate the complementary ssDNA . As discussed above, chemical and protein sensing is evolving toward label‐free detection schemes based on amperometry or SPR.…”