Versatile multispectral microscope based on light emitting diodes

Brydegaard, Mikkel; Merdasa, Aboma; Jayaweera, H. H. E.; Ålebring, Jens; Svanberg, Sune

doi:10.1063/1.3660810

Cited by 35 publications

(34 citation statements)

References 48 publications

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“…Furthermore, the green and red LEDs could be easily replaced with additional excitation bands (e.g., at 445 nm) to evaluate the effects of tissue oxygenation over the UV-visible spectrum 38 and/or acquire multiexcitation fluorescence in order to form a two-dimensional excitation emission matrix. 39 It has the potential to improve cancer diagnostics by providing absorption and fluorescence characteristics of normal and abnormal tissues. 39,40 Point measurements are usually time consuming.…”

Section: Discussionmentioning

confidence: 99%

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Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Xie

Mousavi

et al. 2014

J. Biomed. Opt

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Abstract. Optical techniques for tissue diagnostics currently are experiencing tremendous growth in biomedical applications, mainly due to their noninvasive, inexpensive, and real-time functionality. Here, we demonstrate a hand-held fiber optic probe instrument based on fluorescence/reflectance spectroscopy for precise tumor delineation. It is mainly aimed for brain tumor resection guidance with clinical adaptation to minimize the disruption of the standard surgical workflow and is meant as a complement to the state-of-the-art fluorescence surgical microscopy technique. Multiple light sources with fast pulse modulation and detection enable precise quantification of protoporphyrin IX (PpIX), tissue optical properties, and ambient light suppression. Laboratory measurements show the system is insensitive to strong ambient light. Validation measurements of tissue phantoms using nonlinear least squares support vector machines (LS-SVM) regression analysis demonstrate an error of <5% for PpIX concentration ranging from 400 to 1000 nM, even in the presence of large variations in phantom optical properties. The mean error is 3% for reduced scattering coefficient and 5% for blood concentration. Diagnostic precision of 100% was obtained by LS-SVM classification for in vivo skin tumors with topically applied 5-aminolevulinic acid during photodynamic therapy. The probe could easily be generalized to other tissue types and fluorophores for therapy guidance and monitoring.

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Section: Discussionmentioning

confidence: 99%

“…39 It has the potential to improve cancer diagnostics by providing absorption and fluorescence characteristics of normal and abnormal tissues. 39,40 Point measurements are usually time consuming. However, the probe presented in this work is not intended for tumor delineation for the entire tumor border, especially in open brain surgery.…”

Section: Discussionmentioning

confidence: 99%

Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Xie

Mousavi

et al. 2014

J. Biomed. Opt

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“…Multispectral images of the thin blood smear slides were acquired in transmission mode, using Multispectral Light Emitting Diodes Imaging Microscope system (MSLEDIM), which employed nine light emitting diodes (LEDs), as implemented in Brydegaard et al (Brydegaard, Merdasa, Jayaweera, Ålebring, & Svanberg, 2011). The nine LEDs emit a total of thirteen spectral bands for the system.…”

Section: Multispectral Imagingmentioning

confidence: 99%

Wavelength Markers for Malaria (<em>Plasmodium Falciparum</em>) Infected and Uninfected Red Blood Cells for Ring and Trophozoite Stages

Opoku-Ansah¹,

Eghan

Anderson³

et al. 2014

APR

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Malaria parasite, Plasmodium falciparum, uses haemoglobin in host red blood cells (RBCs) as a major source of nutrient in ring and trophozoite stages. This brings about changes in the morphology and functional characteristics of the RBCs. We investigate malaria infected RBCs and uninfected RBCs-ring and trophozoite stages using multispectral imaging technique. Four spectral bands were found to be markers for identifying infected and uninfected RBCs: 435 nm and 660 nm were common markers for the two stages whiles 590 nm and 625 nm were markers for the ring and the trophozoite stages respectively. These four spectral bands may offer potential diagnostic markers for identifying infected and uninfected RBCs, as well as distinguishing ring and trophozoite stages.

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“…30. In this system, 13 LEDs were used to selectively illuminate the sample at 13 different wavelengths ranging from UV to near infrared (NIR) (380 to 935 nm).…”

Section: Imaging Systemmentioning

confidence: 99%

Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy

et al. 2013

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Abstract. We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for automated imaging, identification, and counting of malaria-infected erythrocytes for real-time and cost-effective parasitaemia diagnosis as an effective alternative to the manual screening of stained blood smears, now considered to be the gold standard in malaria diagnosis. We evaluate the performance of our algorithm against manual estimations of an expert and show a spectrally resolved increased scattering from malaria-infected blood cells. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Versatile multispectral microscope based on light emitting diodes

Cited by 35 publications

References 48 publications

Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Wavelength Markers for Malaria (<em>Plasmodium Falciparum</em>) Infected and Uninfected Red Blood Cells for Ring and Trophozoite Stages

Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy

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