Versatile platform for performing protocols on a chip utilizing surface acoustic wave (SAW) driven mixing

Zhang, Y; Devendran, Citsabehsan; Lupton, Christopher J.; Marco, Alex de; Neild, Adrian

doi:10.1039/c8lc01117f

Cited by 28 publications

(27 citation statements)

References 57 publications

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“…Further, it is widely regarded as biocompatible when operated within the limits determined by the biological matter (Devendran et al 2019). Hence, this manipulation approach has been extensively utilised to mix fluids (Zhang et al 2019;Phan et al 2015) as well as trap (Fakhfouri et al 2016;, pattern and sort droplets (Sesen et al 2017(Sesen et al , 2015 and cells (Collins et al 2015;Ahmed et al 2016).…”

Section: Introductionmentioning

confidence: 99%

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

Devendran

Collins

Neild

2022

Microfluid Nanofluid

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Surface acoustic wave (SAW) micromanipulation offers modularity, easy integration into microfluidic devices and a high degree of flexibility. A major challenge for acoustic manipulation, however, is the existence of a lower limit on the minimum particle size that can be manipulated. As particle size reduces, the drag force resulting from acoustic streaming dominates over acoustic radiation forces; reducing this threshold is key to manipulating smaller specimens. To address this, we investigate a novel excitation configuration based on diffractive-acoustic SAW (DASAW) actuation and demonstrate a reduction in the critical minimum particle size which can be manipulated. DASAW exploits the inherent diffractive effects arising from a limited transducer area in a microchannel, requiring only a travelling SAW (TSAW) to generate time-averaged pressure gradients. We show that these acoustic fields focus particles at the channel walls, and further compare this excitation mode with more typical standing SAW (SSAW) actuation. Compared to SSAW, DASAW reduces acoustic streaming effects whilst generating a comparable pressure field. The result of these factors is a critical particle size with DASAW (1 $$\upmu$$ μ m) that is significantly smaller than that for SSAW actuation (1.85 $$\upmu$$ μ m), for polystyrene particles and a given $$\lambda _{\text {SAW}}$$ λ SAW = 200 $$\upmu$$ μ m. We further find that streaming magnitude can be tuned in a DASAW system by changing the channel height, noting optimum channel heights for particle collection as a function of the fluid wavelength at which streaming velocities are minimised in both DASAW and SSAW devices.

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Section: Introductionmentioning

confidence: 99%

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

Devendran

Collins

Neild

2022

Microfluid Nanofluid

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“…A major reason to use high frequency (30–600 MHz) ultrasound in a microfluidic system is that the wavelength is in the same order as that of a typical cell (i.e., 2–50 µm). This is a prerequisite for patterning of single cells, but has also been shown to provide the possibility of high sensitivity sorting, patterning using either standing waves or traveling waves and fluid mixing protocols . Excitation can be achieved using surface acoustic waves, these waves are substrate bound until they encounter a fluid body whereupon energy is efficiently coupled.…”

Section: System Principlesmentioning

confidence: 99%

“…Acoustofluidics is the use of acoustic forces to manipulate suspended matter within microfluidics, and has the advantage of uniquely combining ease of on‐chip integration and simple, yet dextrous establishment of force fields in a noncontact manner . As a direct result, it has been extensively used to capture, pattern, and sort particles, cell sonoporation, synthesize nanomaterials, as well as to mix fluids . Although acoustofluidics has been widely accepted as a biocompatible technique, substantiated with cell viability studies; there have been no extensive viability studies at elevated frequencies (30–600 MHz).…”

Section: Introductionmentioning

confidence: 99%

Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems

et al. 2019

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As the applications of this technology are focused within the clinical and life sciences, a thorough understanding of the associated biological impact imposed by these manipulation techniques is necessary.The desire to manipulate suspended matter within microfluidic systems has inspired a range of techniques both passive [5,19,20] and active. [21,22] Passive approaches rely heavily on the geometry of the microfluidic channels and inertial forces. Flow profiles are altered by the introduction of sudden expansions and contractions, weirs and pillars to impede and divert the flow into desired streamlines. This reliance on the resultant flow profile restricts the flexibility of the chip, being single task specific. In contrast, active methods are significantly more robust, capable of on-demand actuation and offer the ability to change functionality ad-hoc, leading to an increased selectivity. To this end, a range of active methods have been developed using magnetic, [23,24] optical, [25,26] electrical [16,27] and acoustic [28][29][30] excitation.Acoustofluidics is the use of acoustic forces to manipulate suspended matter within microfluidics, [31,32] and has the advantage of uniquely combining ease of on-chip integration and simple, yet dextrous establishment of force fields in a noncontact manner. [29,33,34] As a direct result, it has been extensively used to capture, [33,35] pattern, [36][37][38] and sort particles, [15,34,39] cell sonoporation, [40] synthesize nanomaterials, [41] as well as to mix fluids. [42,43] Although acoustofluidics has been widely accepted as a biocompatible technique, substantiated with cell viability studies; [44][45][46][47][48] there have been no extensive viability studies at elevated frequencies (30-600 MHz). Indeed, typically studies are corroborated with a single viability method, most commonly live/dead staining, [6,[49][50][51] or trypan blue exclusion [52,53] and in some instances proliferation studies (MTT). [36] In contrast to these singular approaches, in passive microfluidic systems in which cells are predominantly subjected to shear forces arising from the flow field, a wide range of multifaceted cell viability studies [54] have been conducted showing, for example, sheardependent regulation of the von Willebrand factor of human umbilical vein endothelial cells [55] and the potential for circulating tumor cell apoptosis at high shear levels. [56] This lack of biological knowledge may result in potential unrecognized adverse effects (i.e., "false positives"), or Acoustic fields are capable of manipulating biological samples contained within the enclosed and highly controlled environment of a microfluidic chip in a versatile manner. The use of acoustic streaming to alter fluid flows and radiation forces to control cell locations has important clinical and life science applications. While there have been significant advances in the fundamental implementation of these acoustic mechanisms, there is a considerable lack of understanding of the associated biological effects on cells. ...

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“…Acoustic tweezers are a versatile tool that can manipulate particles with sizes between nanometres and centimetres using acoustic waves with frequencies ranged from 1 kHz to 500 MHz [10]. Acoustic tweezers based on surface acoustic wave (SAW) devices have been developed to actuate nano-and micro-particles in the fields of two-dimensional [11] and three-dimensional cell manipulation [12], objects on water [13], protein crystallisation [14], reconfigurable manipulation of particles and cells [15], separation of circulating tumour cells [16] and extracellular vesicles [17][18][19], manipulation of nanoparticles [20], nanoscale confined fluids and particles [21] and droplets [22]. Acoustic tweezers have been integrated with dielectrophoresis [10] and optics [11] to perform delicate noncontact manipulation of various samples.…”

Section: Introductionmentioning

confidence: 99%

Gallium Nitride: A Versatile Compound Semiconductor as Novel Piezoelectric Film for Acoustic Tweezer in Manipulation of Cancer Cells

Sun

Wallis

et al. 2020

IEEE Trans. Electron Devices

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Versatile platform for performing protocols on a chip utilizing surface acoustic wave (SAW) driven mixing

Abstract: We present a dextrous microfluidic device which features a reaction chamber with volume flexibility and acoustic mixing.

Cited by 28 publications

References 57 publications

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices

Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems

Gallium Nitride: A Versatile Compound Semiconductor as Novel Piezoelectric Film for Acoustic Tweezer in Manipulation of Cancer Cells

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