Versuche zur Polyphosphat-�berkompensation in Hefezellen nach Phosphatverarmung

Liss, E; Langen, P.

doi:10.1007/bf00422195

Cited by 61 publications

(35 citation statements)

References 9 publications

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“…PolyP kinase is involved in the synthesis of polyP but probably not in its degradation (29,30,48). The sequential hydrolysis of polyP to the smaller residues, and ultimately to Pi, occurs via polyphosphatase (10), and by alkaline phosphatase, vacuolar enzyme (21).…”

Section: Methodsmentioning

confidence: 99%

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Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.

Bostian¹,

Lemire²,

Halvorson³

1983

Mol. Cell. Biol.

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We have examined the regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [ Phosphorus metabolism in Saccharomyces cerevisiae involves five principal enzymes for the acquisition and metabolic integration of Pi.These enzymes regulate intracellular concentrations of Pi by a cyclic pathway of polyphosphate (polyP) synthesis and degradation (18, 47). The polyP is sequestered into vacuoles and forms the most abundant phosphate compounds in yeasts. The majority is an acid-insoluble polymer of 240,000 daltons, whereas the remainder consists of short-chain-length acid-soluble residues (42).Both a phosphate permease (39) and acid phosphatase (APase) (41) participate in phosphorus acquisition. APase (orthophosphoricmonoester phosphohydrolase [acid optimum], EC 3.1.3.2) is an exocellular enzyme active on a broad spectrum of phosphoester substrates (41).Incorporation of Pi as a monovalent anion occurs by an energy-dependent permease transport mechanism (9, 39). Intracellular Pi is converted via ATP to polyP by the unique enzyme, polyP kinase, which transfers the terminal phosphoryl group of ATP to polyP. Degradation of polyP, on the other hand, is catalyzed by several enzymes, including polyP kinase. However, the direct interconversion of polyP to ATP by polyP kinase does not occur in vivo. Rather, polyP is sequentially hydrolyzed to smaller-chain-length

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Section: Methodsmentioning

confidence: 99%

“…When yeast cells are starved for phosphate, internal reserves of polyP are depleted (49), and overall cellular metabolism becomes dependent upon exocellular APase (41,46 (30), and both APase and alkaline phosphatase are repressed.…”

mentioning

confidence: 99%

Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.

Bostian¹,

Lemire²,

Halvorson³

1983

Mol. Cell. Biol.

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“…In yeast, poly(P) can reach a chain length of more than 260 phosphate units [13]. However, more recently, it has become clear that a specific fraction of poly(P) represents a pool of poly(P) localized in various compartments of the cell [14]; very high molecular mass poly(P) seems to be located at the cell periphery, whereas short chain poly(P) from 4 to 20 residues, were found, at least partly, in the cytoplasm and the nucleus [14].…”

Section: Discussionmentioning

confidence: 99%

Polyphosphates as a source of high energy phosphates in yeast mitochondria: A ³¹P NMR study

et al. 1989

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Suspensions of purified yeast mitochondria were analyzed under bubbling oxygen by IIP NMR at 161.9 MHz. The recorded spectra indicate that polyphosphates (poly(P)) are present in mitochondrial preparations. These poly(P) further characterized by the NMR study of mitochondrial perchloric acid extracts have an average chain length of 14 +_ 1 residues per chain and correspond to 10% of the total content of cellular poly(P) detected by NMR. The stability of mitochondrial poly(P) was increased by the presence of oligomycin, suggesting that this compound may play a role in the energetic metabolism of yeast mitochondria.

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“…The term 'polyphosphate overplus ' is proposed to designate the accumulation of polyphosphate upon addition of orthophosphate to phosphate-starved organisms, in order to distinguish this from polyphosphate accumulation under other conditions of nutrient imbalance (Smith et al 1954). The term corresponds to 'PolyphosphatUberkompensation' as used by Liss & Langen (1962).…”

Section: Introductionmentioning

confidence: 99%

Enzymic and Genetic Control of Polyphosphate Accumulation in Aerobacter aerogenes

Harold

1964

Journal of General Microbiology

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SUMMARYAddition of orthophosphate to Aerobacter aerogezzes strain A 3( 0) organisms previously subjected to phosphate starvation induced accumulation of inorganic polyphosphate within the organisms. With resumption of growth the polyphosphate was degraded and served as a source of nucleic acid phosphorus. During phosphate starvation the specific activity of polyphosphate kinase and inorganic polyphosphatase increased five-to tenfold while the amount of alkaline phosphatase increased 50 times. The results suggest that synthesis of polyphosphate kinase and alkaline phosphatase was subject to repression by exogenous orthophosphate. Two mutant strains blocked in polyphosphate accumulation were found to carry defects in the synthesis of these enzymes. Mutants of class Pn-1 contained normal amounts of all three enzymes, but repression of their synthesis was not annulled by phosphate starvation. Mutants of class Pn-2 contained no polyphosphate kinase. It is suggested that synthesis of polyphosphate kinase is controlled by two genes, a structural gene and a regulator gene; the latter gene also appears to control the synthesis of alkaline phosphatase and perhaps polyphosphatase. The patterns of polyphosphate accumulation under various nutritional conditions are discussed in relation to the amounts and activities of the enzymes of polyphosphate synthesis and degradation.

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Versuche zur Polyphosphat-�berkompensation in Hefezellen nach Phosphatverarmung

Cited by 61 publications

References 9 publications

Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.

Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.

Polyphosphates as a source of high energy phosphates in yeast mitochondria: A ³¹P NMR study

Enzymic and Genetic Control of Polyphosphate Accumulation in Aerobacter aerogenes

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Versuche zur Polyphosphat-�berkompensation in Hefezellen nach Phosphatverarmung

Cited by 61 publications

References 9 publications

Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.

Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae.

Polyphosphates as a source of high energy phosphates in yeast mitochondria: A 31P NMR study

Enzymic and Genetic Control of Polyphosphate Accumulation in Aerobacter aerogenes

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Polyphosphates as a source of high energy phosphates in yeast mitochondria: A ³¹P NMR study