Vertical electric field induced bacterial growth inactivation on amorphous carbon electrodes

Jain, Shilpee; Sharma, Ashutosh; Basu, Bikramjit

doi:10.1016/j.carbon.2014.09.048

Cited by 19 publications

(19 citation statements)

References 36 publications

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“…In another study, a similar observation was made in that cathodic current caused a decline in bacterial attachment on ITO coated glass electrodes while anodic currents promoted bacterial inactivation without affecting adhesion behavior of Pseudomonas . Recent work from our own research group illustrates the antibacterial effect triggered by electric field strength of 2.5 V/cm against S.aureus and E.coli on amorphous carbon substrates . In another work, we demonstrated the synergistic anti‐biofilm action of hydroxyapatite‐zinc oxide (HA‐ZnO) composites and direct current electric field of 1V/cm [Figure (C)], mediated by the production of reactive oxygen species and bacterial membrane depolarization .…”

Section: Strategies Against Prosthetic Infectionsupporting

confidence: 54%

“…115 Recent work from our own research group illustrates the antibacterial effect triggered by electric field strength of 2.5 V/cm against S.aureus and E.coli on amorphous carbon substrates. 116 In another work, we demonstrated the synergistic anti-biofilm action of hydroxyapatite-zinc oxide (HA-ZnO) composites and direct current electric field of 1V/cm [ Figure 8(C)], mediated by the production of reactive oxygen species and bacterial membrane depolarization. 117 The generation of free radicals, reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and reactive nitrogen species (RNS) at the cathode is another experimentally proven mechanism for the bactericidal action of low strength electric field/current.…”

Section: Biophysical Stimulation Methodsmentioning

confidence: 99%

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Engineered biomaterial and biophysical stimulation as combinatorial strategies to address prosthetic infection by pathogenic bacteria

Boda

Basu

2016

J. Biomed. Mater. Res.

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A plethora of antimicrobial strategies are being developed to address prosthetic infection. The currently available methods for implant infection treatment include the use of antibiotics and revision surgery. Among the bacterial strains, Staphylococcus species pose significant challenges particularly, with regard to hospital acquired infections. In order to combat such life threatening infectious diseases, researchers have developed implantable biomaterials incorporating nanoparticles, antimicrobial reinforcements, surface coatings, slippery/non-adhesive and contact killing surfaces. This review discusses a few of the biomaterial and biophysical antimicrobial strategies, which are in the developmental stage and actively being pursued by several research groups. The clinical efficacy of biophysical stimulation methods such as ultrasound, electric and magnetic field treatments against prosthetic infection depends critically on the stimulation protocol and parameters of the treatment modality. A common thread among the three biophysical stimulation methods is the mechanism of bactericidal action, which is centered on biophysical rupture of bacterial membranes, the generation of reactive oxygen species (ROS) and bacterial membrane depolarization evoked by the interference of essential ion-transport. Although the extent of antimicrobial effect, normally achieved through biophysical stimulation protocol is insufficient to warrant therapeutic application, a combination of antibiotic/ROS inducing agents and biophysical stimulation methods can elicit a clinically relevant reduction in viable bacterial numbers. In this review, we present a detailed account of both the biomaterial and biophysical approaches for achieving maximum bacterial inactivation. Summarizing, the biophysical stimulation methods in a combinatorial manner with material based strategies can be a more potent solution to control bacterial infections. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2174-2190, 2017.

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Section: Strategies Against Prosthetic Infectionsupporting

confidence: 54%

Section: Biophysical Stimulation Methodsmentioning

confidence: 99%

Engineered biomaterial and biophysical stimulation as combinatorial strategies to address prosthetic infection by pathogenic bacteria

Boda

Basu

2016

J. Biomed. Mater. Res.

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“…β-Galactosidase is a large, tetrameric protein [ 39 ]. Because of its high molecular weight, presence of extracellular β-galactosidase is generally associated with a loss of cell membrane integrity [ 51 ]. Thus, the activity of extracellular β-galactosidase produced by MEF-treated and untreated cultures was quantified to indicate cell membrane alterations over time.…”

Section: Resultsmentioning

confidence: 99%

“…Intact cell morphology and cytology are essential for the normal growth and multiplication of microorganisms, thus alterations in microbial morphology and cytology reflect cell viability [ 5 , 40 , 51 ]. Microscopic observations of cultures treated for different incubation periods were conducted using SEM and TEM.…”

Section: Resultsmentioning

confidence: 99%

Adaptation response of Pseudomonas fragi on refrigerated solid matrix to a moderate electric field

Chen

Zhang

et al. 2017

BMC Microbiol

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BackgroundModerate electric field (MEF) technology is a promising food preservation strategy since it relies on physical properties—rather than chemical additives—to preserve solid cellular foods during storage. However, the effectiveness of long-term MEF exposure on the psychrotrophic microorganisms responsible for the food spoilage at cool temperatures remains unclear.ResultsThe spoilage-associated psychrotroph Pseudomonas fragi MC16 was obtained from pork samples stored at 7 °C. Continuous MEF treatment attenuated growth and resulted in subsequent adaptation of M16 cultured on nutrient agar plates at 7 °C, compared to the control cultures, as determined by biomass analysis and plating procedures. Moreover, intracellular dehydrogenase activity and ATP levels also indicated an initial effect of MEF treatment followed by cellular recovery, and extracellular β-galactosidase activity assays indicated no obvious changes in cell membrane permeability. Furthermore, microscopic observations using scanning and transmission electron microscopy revealed that MEF induced sublethal cellular injury during early treatment stages, but no notable changes in morphology or cytology on subsequent days.ConclusionOur study provides direct evidence that psychrotrophic P. fragi MC16 cultured on nutrient agar plates at 7 °C are capable of adapting to MEF treatment.

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“…In recent years, research to counteract microbial proliferation in aqueous media has shifted to non‐lethal solutions [Giladi et al, 2008, 2010; Zituni et al, 2014; Jain et al, 2015; Abo‐Neima et al, 2016]. Less penalizing, in terms of energy consumption and pollution, than traditional lethal methods, these new approaches aim to limit or even stop microbial growth by influencing the metabolism of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Distribution of AC Electric Field‐Induced Transmembrane Voltage in Escherichia coli Cell Wall Layers

Rauly

Vindret

Chamberod

et al. 2020

Bioelectromagnetics

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On the basis of Gram-negative bacterium Escherichia coli models previously published in the literature, the transmembrane voltage induced by the application of an alternating current (AC) electric field on a bacterial suspension is calculated using COMSOL Multiphysics software, in the range 1-20 MHz, for longitudinal and transverse field orientations. The voltages developed on each of the three layers of the cell wall are then calculated using an electrical equivalent circuit. This study shows that the overall voltage on the cell wall, whose order of magnitude is a few tens of µV, is mainly distributed on inner and outer layers, while a near-zero voltage is found on the periplasm, due to its much higher electrical conductivity compared with the other layers. Although the outer membrane electrical conductivity taken in the model is a thousand times higher than that of the inner membrane, the voltage there is about half of that on the inner membrane, due to capacitive effects. It follows that the expression of protein complexes anchored in the inner membrane could potentially be disrupted, inducing in particular a possible perturbation of biological processes related to cellular respiration and proton cycle, and leading to growth inhibition as a consequence. Protein complexes anchored in the outer membrane or constituting a bridge between the three layers of the cell wall, such as some porins, may also undergo the same action, which would add another growth inhibition factor, as a result of deficiency in porin filtration function when the external environment contains biocides. Bioelectromagnetics. 2020;41:279-288.

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Vertical electric field induced bacterial growth inactivation on amorphous carbon electrodes

Cited by 19 publications

References 36 publications

Engineered biomaterial and biophysical stimulation as combinatorial strategies to address prosthetic infection by pathogenic bacteria

Engineered biomaterial and biophysical stimulation as combinatorial strategies to address prosthetic infection by pathogenic bacteria

Adaptation response of Pseudomonas fragi on refrigerated solid matrix to a moderate electric field

Distribution of AC Electric Field‐Induced Transmembrane Voltage in Escherichia coli Cell Wall Layers

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