Vertical hydrodynamic focusing in glass microchannels

Lin, Tony An-tong; Hosoi, A. E.; Ehrlich, D. J.

doi:10.1063/1.3055278

Cited by 6 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is particularly useful where the optical detection requires a narrow depthof-field. 38 In a HFF device, cells can for example be confined to a 'thin' flow stream layer, facilitating analysis of single cell events, for example when using fluorescence detection methods.…”

Section: Hydrodynamic Vertical Flow Focussing Device For Spectrophotomentioning

confidence: 99%

condiCharacterisation of an Adhesive-free Packaging System for Polymeric Microfluidic Biochemical Devices and Reactors

Reichen

Super

Davies

et al. 2014

Chem.Biochem.Eng.Q.

View full text Add to dashboard Cite

The development of microfluidic devices is an iterative process that involves series of improvements, which can be costly and time consuming. We present a packaging system which makes use of an accessible rapid prototyping method, and facilitates the rapid and reliable implementation of polymeric microfluidic device designs. The packaging system uses a modular design and is based on an adhesive-free connection of a reusable and stiff polymeric interface plate with a disposable, soft microfluidic chip under compression. We characterised the system by numerically and experimentally studying the effect of compression and key dimensions on burst pressure and flow rate. All parts are fabricated with readily available low-cost materials and micro-milling technology. The presented approach is both facilitating and systematising the fabrication of devices with different degrees of complexity; keeping assembly and interconnection simple and straightforward. Furthermore, minimising the time between a design and a finished working prototype yields rapid verification of microfluidic design concepts and testing of assays. Several chip designs were fabricated, then growth of stem cells and hydrodynamic vertical flow focusing in a microfluidic device were realised using our approach. Our approach minimises the need for re-development and re-testing of interface components; reducing cost and time requirements.

show abstract

Section: Hydrodynamic Vertical Flow Focussing Device For Spectrophotomentioning

confidence: 99%

condiCharacterisation of an Adhesive-free Packaging System for Polymeric Microfluidic Biochemical Devices and Reactors

Reichen

Super

Davies

et al. 2014

Chem.Biochem.Eng.Q.

View full text Add to dashboard Cite

show abstract

Section: Instrument Designmentioning

confidence: 99%

“…9 through two CFD simulation packages (Lin et al, 2009). Based on the resulting models, we constructed simple three-level and four-level vertical focusing devices and tested their performance via 3-D optical imaging in a confocal microscope (Lin et al, 2009). The models show that the profile created by combining two flows in isotropically etched channels progresses nonlinearly as a function of the flow-rate ratio of the several fluid streams.…”

Section: Instrument Designmentioning

confidence: 99%

See 1 more Smart Citation

Parallel Imaging Microfluidic Cytometer

Ehrlich

McKenna

Evans

et al. 2011

Methods in Cell Biology

Self Cite

View full text Add to dashboard Cite

By adding an additional degree of freedom from multichannel flow, the parallel microfluidic cytometer (PMC) combines some of the best features of flow cytometry (FACS) and microscopebased high-content screening (HCS). The PMC (i) lends itself to fast processing of large numbers of samples, (ii) adds a 1-D imaging capability for intracellular localization assays (HCS), (iii) has a high rare-cell sensitivity and, (iv) has an unusual capability for time-synchronized sampling. An inability to practically handle large sample numbers has restricted applications of conventional flow cytometers and microscopes in combinatorial cell assays, network biology, and drug discovery. The PMC promises to relieve a bottleneck in these previously constrained applications. The PMC may also be a powerful tool for finding rare primary cells in the clinic.The multichannel architecture of current PMC prototypes allows 384 unique samples for a cellbased screen to be read out in approximately 6-10 minutes, about 30-times the speed of most current FACS systems. In 1-D intracellular imaging, the PMC can obtain protein localization using HCS marker strategies at many times the sample throughput of CCD-based microscopes or CCD-based single-channel flow cytometers. The PMC also permits the signal integration time to be varied over a larger range than is practical in conventional flow cytometers. The signal-to-noise advantages are useful, for example, in counting rare positive cells in the most difficult early stages of genome-wide screening. We review the status of parallel microfluidic cytometry and discuss some of the directions the new technology may take.

show abstract

“…10,12,[40][41][42][43][44][45][46][47][48][49][50][51][52][53] In these approaches, the key is to develop microfluidic structures to focus particles/cells three-dimensionally. [54][55][56][57][58][59][60][61][62][63][64][65] To this end, we developed a three-dimensional (3D) hydrodynamic focusing technique called "microfluidic drifting." 10,13 By utilizing the Dean flow 65,66 in a curved microfluidic channel, "microfluidic drifting" enables 3D hydrodynamic focusing in a single-layer planar microfluidic device that can be readily fabricated via standard soft-lithography.…”

Section: Introductionmentioning

confidence: 99%

An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing

et al. 2012

View full text Add to dashboard Cite

In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a "microfluidic drifting" based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate onchip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a singlelayer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics. V C 2012 American Institute of Physics. [http://dx

show abstract

Vertical hydrodynamic focusing in glass microchannels

Cited by 6 publications

References 10 publications

condiCharacterisation of an Adhesive-free Packaging System for Polymeric Microfluidic Biochemical Devices and Reactors

condiCharacterisation of an Adhesive-free Packaging System for Polymeric Microfluidic Biochemical Devices and Reactors

Parallel Imaging Microfluidic Cytometer

An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing

Contact Info

Product

Resources

About