Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits

Robinson, Jacob T.; Jorgolli, Marsela; Shalek, Alex K.; Yoon, Myung‐Han; Gertner, Rona S.; Park, Hongkun

doi:10.1038/nnano.2011.249

Cited by 541 publications

(610 citation statements)

References 32 publications

Supporting

Mentioning

599

Contrasting

Unclassified

Order By: Relevance

“…Ti/TiN base electrodes with different diameters are covered by a sacrificial amorphous silicon (a-Si) layer. This layer is used as a template and structured via deep reactive ion etching (DRIE) with alternating fluxes of C 4 F 8 and SF 6 for passivation and etching respectively. Thus, a structure width of about 400 nm and an aspect ratio of about 1:7.5 can be achieved [11].…”

Section: Fabrication Processmentioning

confidence: 99%

“…If the electrodes are sufficiently small -i.e. if their diameter is in the order of 200 nm -they can penetrate the cell membrane and hence measure intracellular signals [6] [7]. This results in an improved electrical coupling and enables the detection of sub-threshold events [6] [7].…”

Section: Introductionmentioning

confidence: 99%

“…if their diameter is in the order of 200 nm -they can penetrate the cell membrane and hence measure intracellular signals [6] [7]. This results in an improved electrical coupling and enables the detection of sub-threshold events [6] [7]. By the integration of penetrating electrodes on a CMOS IC, the advantages of the sensitive intracellular contacts and the programmable recording and stimulation are combined.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication and electrochemical characterization of ruthenium nanoelectrodes

Allani

Jupe

Figge

et al. 2017

Current Directions in Biomedical Engineering

View full text Add to dashboard Cite

Abstract:The Fraunhofer IMS has recently developed a technique for producing nanoelectrodes that are generated by atomic layer deposition (ALD) in a via deep reactive ion etching (DRIE) structured sacrificial layer. This method enables the fabrication of CMOS-and biocompatible nanoelectrodes with suitable ALD-materials. Improvements of the established fabrication processes and the electrochemical characterization of such electrodes are presented. In the frame of the Fraunhofer-Max-Planckcooperation project ZellMOS different types of nanoelectrodes are studied. Their diameter is in the range of 200 nm and thereby sufficiently small to be taken up by living cells. In addition, the electrodes are mechanically enforced by an oxide layer at the nanoelectrodes' bottom.

show abstract

Section: Fabrication Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication and electrochemical characterization of ruthenium nanoelectrodes

Allani

Jupe

Figge

et al. 2017

Current Directions in Biomedical Engineering

View full text Add to dashboard Cite

show abstract

“…The proposed technique can be engaged along with the current state-of-the-art in monitoring intracellular potential recordings. 15,16 An indicative example would be the application with Utah electrode arrays, in which ITO deposition on the active electrode tips before parylene encapsulation would finally yield a 3-D chemical sensing array. 17 The fabricated sensor has been characterized with the aid of discrete devices based on the electrostatic coupling between the H þ and the insulating membrane.…”

Section: -mentioning

confidence: 99%

Sensing H+ with conventional neural probes

Trantidou

Prodromakis

Tsiligkiridis

et al. 2013

Applied Physics Letters

View full text Add to dashboard Cite

In this paper, we demonstrate a technique for transforming commercially available neural probes used for electrical recordings, into chemical sensing devices for detection of ionic concentrations in electrolytes, with particular emphasis to pH. This transformation requires a single post-processing step to incorporate a thin indium tin oxide membrane for sensing H+. Measured results indicate a chemical sensitivity of 28 mV/pH, and relatively low leakage currents (2–10 nA) and drifts (1–10 mV/h). The proposed sensing device demonstrates the possibility of a low-cost implementation that can be reusable and thus versatile, with potential applications in real-time extracellular but mainly intracellular chemical monitoring.

show abstract

“…In practice, this involves transiently rapturing the cell membrane by applying a zap voltage to a nanostructured electrode, thus allowing the electrodes to enter the cell and perform pseudo-intracellular recording until the raptured membrane reorganizes. 1,12,13 In this letter, we propose an alternative approach to increase the neuron-electrode seal for amplifying signals in extracellular recordings. This is achieved by covering neurons with an electrically resistive sheet.…”

mentioning

confidence: 99%

An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings

Matsumura

Yamamoto

Niwano

et al. 2016

Applied Physics Letters

View full text Add to dashboard Cite

Electrical signals of neuronal cells can be recorded non-invasively and with a high degree of temporal resolution using multielectrode arrays (MEAs). However, signals that are recorded with these devices are small, usually 0.01%-0.1% of intracellular recordings. Here, we show that the amplitude of neuronal signals recorded with MEA devices can be amplified by covering neuronal networks with an electrically resistive sheet. The resistive sheet used in this study is a monolayer of glial cells, supportive cells in the brain. The glial cells were grown on a collagen-gel film that is permeable to oxygen and other nutrients. The impedance of the glial sheet was measured by electrochemical impedance spectroscopy, and equivalent circuit simulations were performed to theoretically investigate the effect of covering the neurons with such a resistive sheet. Finally, the effect of the resistive glial sheet was confirmed experimentally, showing a 6-fold increase in neuronal signals. This technique feasibly amplifies signals of MEA recordings. V C 2016 AIP Publishing LLC. Microelectrode array (MEA) technology is widely used to record trains of action potentials from cultured neurons, cardiomyocytes, or brain slice preparations. [1][2][3][4] The major advantage of this method lies in its high temporal resolution (>10 kHz) and non-invasiveness. MEA recordings have been used both in fundamental studies, e.g., to analyse activity patterns of cultured neuronal networks, 5,6 and in pharmacological research, for screening lead compounds in vitro. 7,8 However, small signals that can be detected through extracellularly positioned microelectrodes inhibit MEAs from being used in further applications such as studies of subthreshold activity. The amplitude of extracellularly recorded signals is usually in the order of ten to a hundred microvolts, which is 3-4 orders of magnitude lower than the intracellular change of membrane potential in an action potential ($100 mV).Various attempts have been made to overcome this issue. One approach involves increasing the seal resistance between the cell and the electrode. This can be achieved by using nanostructured electrodes instead of planar electrodes that become engulfed by overlying cells. 1,9 A second approach is to decrease the electrode impedance. To record activity from single cells, the use of a smaller electrode is preferable, but this comes at the cost of increased electrode impedance, which decreases the signal. Wolfrum et al. overcame this dilemma by creating micropores that interface the cells to larger electrodes. 10,11 A third approach involves decreasing the membrane impedance at the cell-electrode junction. In practice, this involves transiently rapturing the cell membrane by applying a zap voltage to a nanostructured electrode, thus allowing the electrodes to enter the cell and perform pseudo-intracellular recording until the raptured membrane reorganizes. 1,12,13 In this letter, we propose an alternative approach to increase the neuron-electrode seal for amplifying signals in ex...

show abstract

Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits

Cited by 541 publications

References 32 publications

Fabrication and electrochemical characterization of ruthenium nanoelectrodes

Fabrication and electrochemical characterization of ruthenium nanoelectrodes

Sensing H+ with conventional neural probes

An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings

Contact Info

Product

Resources

About