Vertical Profiles of Community Abundance and Diversity of Anaerobic Methanotrophic Archaea (ANME) and Bacteria in a Simple Waste Landfill in North China

Dong, Jun; Ding, Linjie; Wang, Xu; Chi, Zifang; Lei, Junfeng

doi:10.1007/s12010-014-1456-3

Cited by 27 publications

(17 citation statements)

References 36 publications

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“…oxyfera -like bacteria have also been retrieved from sediments in the South China Sea, which confirmed that they are distributed in the continental shelves, slopes and abysses [ 23 ]. Further study has been conducted by investigating the vertical distribution of anaerobic methanotrophic archaea (ANME) and bacteria in a simple waste landfill in North China [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Fingerprint and Dominant Environmental Factors of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in Sediments from the Yellow River Estuary, China

Yan

Wei

et al. 2015

PLoS ONE

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Nitrite-dependent anaerobic methane oxidation (n-damo) is performed by “Candidatus Methylomirabilis oxyfera” (M. oxyfera), which connects the carbon and nitrogen global nutrient cycles. In the present study, M. oxyfera-like bacteria sequences were successfully recovered from Yellow River Estuary sediments using specific primers for 16S rRNA and pmoA genes. A M. oxyfera-like sequences analysis based on the 16S rRNA gene revealed greater diversity compared with the pmoA gene; the 16S rRNA gene sequences retrieved from the Yellow River Estuary sediments belong to groups A as well as B and were mainly found in freshwater habitats. Quantitative PCR showed that 16S rRNA gene abundance varied from 9.28±0.11×103 to 2.10±0.13×105 copies g-1 (dry weight), and the pmoA gene abundance ranged from 8.63±0.50×103 to 1.83±0.18×105 copies g-1 (dry weight). A correlation analysis showed that the total organic carbon (TOC) and ammonium (NH4 +) as well as the ratio of total phosphorus to total nitrogen (TP/TN) influenced the M. oxyfera-like bacteria distribution in the Yellow River Estuary sediments. These findings will aid in understanding the n-damo bacterial distribution pattern as well as their correlation with surrounding environmental factors in temperate estuarine ecosystems.

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Section: Introductionmentioning

confidence: 99%

Molecular Fingerprint and Dominant Environmental Factors of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in Sediments from the Yellow River Estuary, China

Yan

Wei

et al. 2015

PLoS ONE

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“…Researchers also discovered methanotroph from soil and sewage sediments of landfill sites [16,17] and some researchers found anaerobic archaea and germs [18,19] , which is rarely found in other conventional natural environments.…”

Section: Conventional Natural Environmentmentioning

confidence: 99%

“…Researchers demonstrate that the anaerobic oxidation of methane occurs based on two approaches [32,33] : on the one hand, it is conducted under the drive of methanotroph and the symbiont of sulfate reducting bacteria; on the other hand, it is carried out by using methanotroph and nitrate denitrification. Most anaerobic methanotroph is detected from ocean sediments and researchers have also detected anaerobic methanotroph from soil and sewage depositions of landfill sites [18,19] in recent years. Min Hang et al in Zhejiang University in China found the bacteria strain AMOB3 in rice fields which can cause the anaerobic oxidation of methane and suggest that the bacteria strain is a new species which differs from existing methanotroph.…”

Section: Distributions Of Anaerobic Methanotrophmentioning

confidence: 99%

Ecological Distribution and Application Research Status of Methanotroph B Ased on Gas Control of Coal Mines

Wu¹,

Zhang²,

Zhang³

et al. 2017

Topics in Civil, Environmental &Amp; Building Engineering (TICEBE)

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Gas disaster is considered as a severe problem faced by underground mines. It is seen as a feasible technological means to degrade gases in coal mines by using methanotroph, which is although subjected to a primary research stage in application. Researchers sampled, cultured and identified multiple effective methanotrophs in conventional and extreme natural environments as well as production environment of coal mines. However, the majority of methanotroph is aerobic while minority of them is anaerobic. The designed experimental conditions and scales in the research significantly differed from the actual conditions of coal seams so that it is hard to apply the research on site. This study analyzed the major problems of current researches and put forward the requirement in environmental conditions: the actual conditions of gases of coal mines are fully taken into consideration in the future researches. Moreover, the study pointed out that the extraction environment and cultured conditions of effective methanotroph and is expected to concentrate on improving research efficiency and application value.

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“…It was of interest to investigate whether or not the BHO1 strain contains which form of MMO. To answer this question, PCR-amplification of two marker genes which are essential in detecting MMO: mmoX (a gene encoding a subunit of soluble methane monooxygenase) and pmoA (a gene encoding a subunit of particulate methane monooxygenase) (Dong et al, 2015) was carried out using the specific primers. Amplification of pmoA with primers A189f/mb661r using the recommended PCR conditions (Holmes et al, 1995, Costello and Lidstrom, 1999) resulted in a product of about 500 bp length as expected (Fig.…”

Section: S Rrna Gene Sequence Analysis and Phylogenetic Treementioning

confidence: 99%

“…All methanotrophs contain either sMMO or pMMO while only some of them contain both forms. Two marker genes are essential in detecting MMO: mmoX (a gene encoding a subunit of soluble methane monooxygenase) and pmoA (a gene encoding a subunit of particulate methane monooxygenase) (Dong et al, 2015). The sMMO and pMMO play a major role in biotechnological application via their ability to oxidize many halogenated hydrocarbon.…”

Section: Introductionmentioning

confidence: 99%

Description of a methanotrophic strain BOH1, isolated from Al-Bohyriya well, Al-Ahsa City, Saudi Arabia

Almalki

Khalifa

2017

Saudi Journal of Biological Sciences

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Methanotrophic bacteria have a unique ability to utilize methane as their carbon and energy sources. Therefore, methanotrophs play a key role in suppressing methane emissions from different ecosystems and hence in alleviating the global climate change. Despite methanotrophs having many ecological, economical and biotechnological applications, little is known about this group of bacteria in Al-Ahsa. Therefore, the main objective of the current work was to expand our understanding of methane oxidizing bacteria in Al-Ahsa region. The specific aim was to describe a methanotrophic strain isolated from Al-Bohyriya well, Al-Ahsa using phenotypic, genotypic (such as 16S rRNA and pmoA gene sequencing) and phylogenetic characterization. The results indicated that the strain belongs to the genus Methylomonas that belongs to Gammaproteobacteria as revealed by the comparative sequence analysis of the 16S rRNA and pmoA genes. There is a general agreement in the profile of the phylogenetic trees based on the sequences of 16srRNA and pmoA genes of the strain BOH1 indicating that both genes are efficient taxonomic marker in methanotrophic phylogeny. The strain possesses the particulate but not the soluble methane monooxygenase as a key enzyme for methane metabolism. Further investigation such as DNA:DNA hybridization is needed to assign the strain as a novel species of the genus Methyomonas and this will open the door to explore the talents of the strain for its potential role in alleviating global warming and biotechnological applications in Saudi Arabia such as bioremediation of toxic by-products released in oil industry. In addition, the strain enhances our knowledge of methanotrophic bacteria and their adaptation to desert ecosystems.

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Vertical Profiles of Community Abundance and Diversity of Anaerobic Methanotrophic Archaea (ANME) and Bacteria in a Simple Waste Landfill in North China

Cited by 27 publications

References 36 publications

Molecular Fingerprint and Dominant Environmental Factors of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in Sediments from the Yellow River Estuary, China

Molecular Fingerprint and Dominant Environmental Factors of Nitrite-Dependent Anaerobic Methane-Oxidizing Bacteria in Sediments from the Yellow River Estuary, China

Ecological Distribution and Application Research Status of Methanotroph B Ased on Gas Control of Coal Mines

Description of a methanotrophic strain BOH1, isolated from Al-Bohyriya well, Al-Ahsa City, Saudi Arabia

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