Vertical toxoplasmosis in a murine model. Protection after immunization with antigens of Toxoplasma gondii incorporated into liposomes

Elsaid, Mohamed M. A.; Martins, Marta; Frézard, Frédéric; Braga, Érika Martins; Vítor, Ricardo Wagner Almeida

doi:10.1590/s0074-02762001000100011

Cited by 29 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tachyzoite antigen -Soluble tachyzoite antigens (STAg) were obtained as previously described (Elsaid et al 2001). Tachyzoites of the RH strain were sonicated using five periods of 30 sec at 40 hertz at 1 min intervals.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-³ and IL-10

Brandão¹,

Melo²,

Gazzinelli

et al. 2009

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

“…Sera were tested individually for determination of IgA, IgM and IgG (total IgG, IgG1 and IgG2a) by ELISA, according to Elsaid et al (2001). Microplates were coated with STAg (0,5 μg/well).…”

Section: Enzyme-linked Immunosorbent Assay (Elisa) -mentioning

confidence: 99%

Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-³ and IL-10

Brandão¹,

Melo²,

Gazzinelli

et al. 2009

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…Furthermore, other experimental studies document congenital transmission in mice (e.g. Elsaid et al 2001 ;Stahl, Sekiguchi & Kaneda, 2002). However, the frequency of vertical transmission in natural populations of mammals has only been addressed in one study to date.…”

Section: Introductionmentioning

confidence: 99%

Detection of high levels of congenital transmission of Toxoplasma gondii in natural urban populations of Mus domesticus

et al. 2004

View full text Add to dashboard Cite

The relative importance of different transmission routes of Toxoplasma gondii has been a matter for debate. This ubiquitous parasite is generally thought to be transmitted by infective oocysts excreted by the definitive host, the cat. Ingestion of undercooked meat has also been considered an important route of transmission in many mammals while congenital transmission has generally been considered relatively rare. Experimental studies demonstrate the ability of T. gondii to be transmitted congenitally, but few studies have investigated the frequency of this transmission route in natural populations. We use PCR amplification of the SAG1 gene to investigate the frequency of congenital transmission in a wild population of mice (Mus domesticus) and show that congenital transmission is occurring in 75% of pregnancies in this population. Furthermore, for infected pregnant mice, transmission occurs to at least one foetus in 100% of cases while variable penetrance of congenital infection is observed. These high levels of congenital transmission in this wild population of mice, taken together with other recent data on congenital transmission in sheep, suggests that this phenomenon might be more widespread than previously thought.

show abstract

“…Thirty days after inoculation, each surviving mouse was bled via retro-orbital plexus. The blood (0.1 ml) was centrifuged, and the plasma was used to perform enzyme-linked immunosorbent assay (ELISA) for anti-T. gondii IgG antibodies (15). Animals that died before 30 days postinoculation were examined for the presence of tachyzoites in the peritoneum or cysts in the brain.…”

mentioning

confidence: 99%

Genetic Characterization of Toxoplasma gondii Revealed Highly Diverse Genotypes for Isolates from Newborns with Congenital Toxoplasmosis in Southeastern Brazil

et al. 2013

View full text Add to dashboard Cite

e Recent studies of Toxoplasma gondii isolates from animals in Brazil have revealed high genetic diversity. Many of these isolates are virulent to mice. It is speculated that these isolates may also be virulent to humans. However, there is very limited data regarding T. gondii strains from human infection. Therefore, it is not clear whether there is any association between parasite genotypes and disease phenotypes. In this study, a total of 27 T. gondii strains were isolated from humans with congenital toxoplasmosis in Minas Gerais state, Brazil. The genetic variability was assessed by restricted fragment length polymorphism in 11 loci (SAG1, 5= plus 3= SAG2, alternative [alt.] SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). Genetic analysis of 24 strains revealed 14 different genotypes, including 7 previously identified from animals and 7 new types. The widespread genotype BrII accounted for 29% (7/24) of the isolates and was the dominant genotype involved in this study. This is the first report of genotyping of T. gondii isolates obtained from blood samples from newborns with congenital toxoplasmosis. Genotypic characterization of these isolates suggests high genetic diversity of T. gondii in this human population in Brazil. Future studies are needed to determine the source of contamination of this human population.

show abstract

Vertical toxoplasmosis in a murine model. Protection after immunization with antigens of Toxoplasma gondii incorporated into liposomes

Cited by 29 publications

References 19 publications

Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-³ and IL-10

Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-³ and IL-10

Detection of high levels of congenital transmission of Toxoplasma gondii in natural urban populations of Mus domesticus

Genetic Characterization of Toxoplasma gondii Revealed Highly Diverse Genotypes for Isolates from Newborns with Congenital Toxoplasmosis in Southeastern Brazil

Contact Info

Product

Resources

About