Vertical transmission of nucleopolyhedrovirus in the silkworm, Bombyx mori L

Khurad, Arun M.; Mahulikar, A.; Rathod, M. K.; Rai, M.M.; Kanginakudru, Sriramana; Nagaraju, Javaregowda

doi:10.1016/j.jip.2004.05.008

Cited by 55 publications

(47 citation statements)

References 13 publications

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“…Histopathology studies have confirmed the presence of virions in rudimentary spermatocytes and oocytes in the developing gonads in late-instar Bombyx mori larvae, and mating between healthy and infected adults resulted in reduced fecundity, possibly due to improper oocyte vitellogenesis (17). As observed in the present study, egg hatchability (fertility) was also adversely affected following OB treatments in other pest moth species (22,27), which together with reduced fecundity, further lowers the reproductive capacity of these insects.…”

Section: Discussionsupporting

confidence: 83%

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Intra- and Intergenerational Persistence of an Insect Nucleopolyhedrovirus: Adverse Effects of Sublethal Disease on Host Development, Reproduction, and Susceptibility to Superinfection

Cabodevilla

Villar

Virto

et al. 2011

Appl Environ Microbiol

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Sublethal infections by qPCR analysis indicated a consistently higher prevalence of sublethal infection than RT-PCR.Sublethal infection was associated with significant reductions in pupal weight, adult emergence, fecundity, and fertility (egg hatch) and significant increases in larval development time and duration of the preoviposition period. Insects taken from a persistently infected experimental population were significantly more susceptible to the OB inoculum than control insects that originated from the same virus-free colony as the persistently infected insects. We conclude that OB treatment results in rapid establishment of sublethal infections that persist between generations and which incur costs in the development and reproductive capacity of the host insect.

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Section: Discussionsupporting

confidence: 83%

“…A growing body of molecular evidence indicates that insect pathogenic viruses can persist in adult insects that were sublethally infected as larvae (2,17,39). Molecular techniques have been based on the detection of viral DNA or mRNA transcripts (3,(11)(12)(13)32) or, more recently, proteomics-based techniques (1).…”

mentioning

confidence: 99%

Intra- and Intergenerational Persistence of an Insect Nucleopolyhedrovirus: Adverse Effects of Sublethal Disease on Host Development, Reproduction, and Susceptibility to Superinfection

Cabodevilla

Villar

Virto

et al. 2011

Appl Environ Microbiol

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“…The second group, silkworms were inoculated with the bacteria by smearing the bacterial solution (7 × 10 7 cells/ml) on a known weight (~20 g) of mulberry leaf surface and dried in shade then fed to larvae. The same procedure was done for viral infection with polyhedral occlusion bodies (OBs) (2 × 10 5 OBs/ml) (the third group) as recommended by Khurad et al (2004). The control group (first group) was fed with distilled water smeared mulberry leaves.…”

Section: Pathogen Inoculationsmentioning

confidence: 99%

Metabolic profiles of pathogen-challenged mulberry silkworm, Bombyx mori L. as a tool for disease diagnosis

Taha¹,

Ismail²,

Abdel-Gawad³

2018

Biochem Biotechnol Res

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Metabolic profiles were detected in healthy and flacherie (inoculated with Bacillus sp.) and grasserie (inoculated with polyhedral bodies)-diseased Bombyx mori larvae. Free amino acids were separated and quantitatively determined by using hydrolysis method. Further, nitrogen (N) percentage was estimated with micro-Kieldahl method. Whereas, phosphor-molybdate method was applied for phosphorus (P) analysis. Results revealed that distinct differences in the amounts of eighteen individual amino acids in healthy and diseased silkworm 5 th larval instar were found. The highest amino acids concentration was recorded in healthy larvae (224.05 mg/g tissue), while the lowest concentration was found in Gr-diseased larvae (177.77 mg/g tissue). Fl-diseased larvae inoculated with Bacillus sp. showed elevation in certain amino acids comparing with Gr-diseased larvae inoculated with of Nuclear Polyhedrosis Virus (NPV) occluded bodies. N and P percentages in Fl and Gr diseases recorded (N = 2.57%, P = 0.29%), (N = 3.86%, P = 0.32%) respectively, compared with healthy larvae (N = 1.63%, P = 0.23%). The present study reveals that a decrease in amino acids in fifth-larval stage implies an increase in N and P percentages in both bacterial and viral infected larvae. It is concluded that the determination of biochemical responses in silkworm against infections may be considered as bio-analytical markers, which are useful for early disease diagnosis and developing disease resistant breeds.

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“…To prepare occlusion bodies for the infection of the B. mori larvae, the transgenic BmN cells were infected with the polh gene negative viruses recombinant baculoviruses BmPAK6 (the mutant virus with lac Z gene instead of polh gene) and BmGFP (the mutant virus with gfp gene instead of polh gene) at 27°C, respectively [16]. Seventy-two hours after infection, virus-infected cells were observed using fluorescent microscopy.…”

Section: Recombinant Virus Infection In the Transgenic Bmn Cellsmentioning

confidence: 99%

The Transgenic BmN Cells with Polyhedrin Gene: A Potential Way to Improve the Recombinant Baculovirus Infection Per Os to Insect Larvae

Shen²,

et al. 2008

Appl Biochem Biotechnol

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The principle of baculovirus expression system is that substitute exogenous gene for polyhedrin (polh) gene, and the recombinant baculovirus lacks the ability to infect insect larvae by oral inoculation. In this study, we cloned the polh gene with immediate early gene 1 (ie1) promoter of Bombyx mori nucleopolyhedrovirus (BmNPV) into transposon pigA3GFP vector, transported it into BmN cells by lipofectamine and obtained the transgenic BmN cell line. The mRNA transcription of the polyhedrin gene was demonstrated by reverse transcription-polymerase chain reaction. Then the polh gene negative viruses (BmPAK6 and BmGFP), infected the transgenic BmN cells and Polyhedrin-like structures were observed in the infected cells. Subsequently, the viruses (vBmPAK6 and vBmGFP) from infected cells were used to orally inoculated the fifth instar larvae of B. mori, respectively. The results showed that B. mori larvae could be infected per os with the recombinant baculoviruses vBmPAK6 and vBmGFP, respectively. These results suggest that the products of polh gene expressed in the transgenic BmN cells could package the recombinant baculoviruses when the viruses infected the cells and raise the pathogenicity of the recombinant virus in orally infected B. mori larvae.

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Vertical transmission of nucleopolyhedrovirus in the silkworm, Bombyx mori L

Cited by 55 publications

References 13 publications

Intra- and Intergenerational Persistence of an Insect Nucleopolyhedrovirus: Adverse Effects of Sublethal Disease on Host Development, Reproduction, and Susceptibility to Superinfection

Intra- and Intergenerational Persistence of an Insect Nucleopolyhedrovirus: Adverse Effects of Sublethal Disease on Host Development, Reproduction, and Susceptibility to Superinfection

Metabolic profiles of pathogen-challenged mulberry silkworm, Bombyx mori L. as a tool for disease diagnosis

The Transgenic BmN Cells with Polyhedrin Gene: A Potential Way to Improve the Recombinant Baculovirus Infection Per Os to Insect Larvae

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