Very long-chain-fatty acids enhance adipogenesis through coregulation of Elovl3 and PPARγ in 3T3-L1 cells

Kobayashi, Takeshi; Fujimori, Ko

doi:10.1152/ajpendo.00623.2011

Cited by 52 publications

(38 citation statements)

References 45 publications

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“…Because unsaturated fatty acids and other lipids have been shown to modulate the expression of several genes such as TAG lipases and others through the activation of the nuclear receptors PPARs (17,30,46), we next tested the hypothesis that these receptors are involved in the positive effects of palmitoleic acid on lipolysis. Accordingly, pharmacological inhibition or genetic deletion of PPAR␣, but not PPAR␥, completely abolished the positive modulation of lipolysis and lipase expression induced by palmitoleic acid.…”

Section: Discussionmentioning

confidence: 99%

Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPARα-dependent manner

Lopes

Festuccia

Farias

et al. 2013

American Journal of Physiology-Endocrinology and Metabolism

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We investigated whether palmitoleic acid, a fatty acid that enhances whole body glucose disposal and suppresses hepatic steatosis, modulates triacylglycerol (TAG) metabolism in adipocytes. For this, both differentiated 3T3-L1 cells treated with either palmitoleic acid (16:1n7, 200 μM) or palmitic acid (16:0, 200 μM) for 24 h and primary adipocytes from wild-type or PPARα-deficient mice treated with 16:1n7 (300 mg·kg(-1)·day(-1)) or oleic acid (18:1n9, 300 mg·kg(-1)·day(-1)) by gavage for 10 days were evaluated for lipolysis, TAG, and glycerol 3-phosphate synthesis and gene and protein expression profile. Treatment of differentiated 3T3-L1 cells with 16:1n7, but not 16:0, increased basal and isoproterenol-stimulated lipolysis, mRNA levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and protein content of ATGL and pSer(660)-HSL. Such increase in lipolysis induced by 16:1n7, which can be prevented by pharmacological inhibition of PPARα, was associated with higher rates of PPARα binding to DNA. In contrast to lipolysis, both 16:1n7 and 16:0 increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose without affecting glyceroneogenesis and glycerokinase expression. Corroborating in vitro findings, treatment of wild-type but not PPARα-deficient mice with 16:1n7 increased primary adipocyte basal and stimulated lipolysis and ATGL and HSL mRNA levels. In contrast to lipolysis, however, 16:1n7 treatment increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose in both wild-type and PPARα-deficient mice. In conclusion, palmitoleic acid increases adipocyte lipolysis and lipases by a mechanism that requires a functional PPARα.

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Section: Discussionmentioning

confidence: 99%

Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPARα-dependent manner

Lopes

Festuccia

Farias

et al. 2013

American Journal of Physiology-Endocrinology and Metabolism

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“…Oil-Red O staining was performed as described previously [28]. Quantification of triglyceride (TG) using the TG E-test Kit (Wako) was performed as described previously [29].…”

Section: T3-l1 Adipocyte Cell Culturementioning

confidence: 99%

13‐Oxo‐9(Z),11(E),15(Z)‐octadecatrienoic Acid Activates Peroxisome Proliferator‐Activated Receptor γ in Adipocytes

Takahashi

Hara

Goto

et al. 2014

Lipids

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Peroxisome proliferator-activated receptor (PPAR)γ is expressed in adipose tissue and plays a key role in the regulation of adipogenesis. PPARγ activators are known to have potent antihyperglycemic activity and are used to treat insulin resistance associated with diabetes. Therefore, many natural and synthetic agonists of PPARγ are used in the treatment of glucose disorders. In the present study, we found that 13-oxo-9(Z),11(E),15(Z)-octadecatrienoic acid (13-oxo-OTA), a linolenic acid derivative, is present in the extract of tomato (Solanum lycopersicum), Mandarin orange (Citrus reticulata), and bitter gourd (Momordica charantia). We also found that 13-oxo-OTA activated PPARγ and induced the mRNA expression of PPARγ target genes in adipocytes, thereby promoting differentiation. Furthermore, 13-oxo-OTA induced secretion of adiponectin and stimulated glucose uptake in adipocytes. To our knowledge, this is the first study to report that 13-oxo-OTA induces adipogenesis through PPARγ activation and to present 13-oxo-OTA as a valuable food-derived compound that may be applied in the management of glucose metabolism disorders.

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“…activity that seems to be due to a reduction of 25% in cellular ATP levels associated to an 352 increase in the activity of acyl-CoA synthase and fatty acid reesterification into TAG (17).…”

Section: Ppard Mediates the Effects Of Desnutrin-catalyzed Lipolysis mentioning

confidence: 99%

“…Estudos in vitro e in vivo (roedores e humanos) encontraram que o ácido palmitoleico possui ações importantes no músculo esquelético e fígado aumentando a captação de glicose e a sensibilidade à insulina, bem como reduzindo a esteatose hepática, respectivamente (1,(16)(17)(18). Mais especificamente, estudos verificaram que células musculares esqueléticas tratadas com ácido palmitoleico apresentam aumento na captação de glicose, maior conteúdo proteico de transportadores de glicose 1 e 4 (GLUTs) na membrana celular e aumento na fosforilação da AKT, do receptor de insulina (IR) e dos substratos do receptor de insulina 1 -2 (IRS), além de aumento na oxidação de glicose (1,17,19).…”

Section: Introductionunclassified

“…Mais especificamente, estudos verificaram que células musculares esqueléticas tratadas com ácido palmitoleico apresentam aumento na captação de glicose, maior conteúdo proteico de transportadores de glicose 1 e 4 (GLUTs) na membrana celular e aumento na fosforilação da AKT, do receptor de insulina (IR) e dos substratos do receptor de insulina 1 -2 (IRS), além de aumento na oxidação de glicose (1,17,19). Interessantemente, Dimopoulos et al (2006) demonstraram que o tratamento com ácido palmitoleico bloqueou os efeitos deletérios do ácido palmítico no metabolismo da glicose e sensibilidade à insulina em células musculares L6 (17).…”

Section: Introductionunclassified

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Efeitos do ácido palmitoleico na captação e metabolismo de glicose e triacilglicerol em adipócitos brancos.

Lopes¹

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Very long-chain-fatty acids enhance adipogenesis through coregulation of Elovl3 and PPARγ in 3T3-L1 cells

Abstract: Kobayashi T, Fujimori K. Very long-chain-fatty acids enhance adipogenesis through coregulation of Elovl3 and PPAR␥ in 3T3-L1 cells.

Cited by 52 publications

References 45 publications

Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPARα-dependent manner

Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPARα-dependent manner

13‐Oxo‐9(Z),11(E),15(Z)‐octadecatrienoic Acid Activates Peroxisome Proliferator‐Activated Receptor γ in Adipocytes

Efeitos do ácido palmitoleico na captação e metabolismo de glicose e triacilglicerol em adipócitos brancos.

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