Very rapid induction of a cold shock protein by temperature downshift in Thermus thermophilus

Mega, Ryosuke; Manzoku, Miho; Shinkai, Akeo; Nakagawa, Noriko; Kuramitsu, Seiki; Masui, Ryoji

doi:10.1016/j.bbrc.2010.07.065

Cited by 24 publications

(31 citation statements)

References 33 publications

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“…It was previously reported that expression of tthb071 was dramatically up-regulated when wild-type T. thermophilus HB8 was subjected to a rapid temperature downshift (from 70 to 45°C) (41). In our oxidative stress experiments, we could not exclude the possibility that cells were exposed to such a cold shock during experimental manipulations.…”

Section: Resultsmentioning

confidence: 56%

In Vivo, in Vitro, and X-ray Crystallographic Analyses Suggest the Involvement of an Uncharacterized Triose-phosphate Isomerase (TIM) Barrel Protein in Protection against Oxidative Stress

Nakane

Wakamatsu

Masui

et al. 2011

Journal of Biological Chemistry

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Background: Characterization of conserved hypothetical proteins is essential for finding novel biological phenomena. Results: A hypothetical protein, TTHB071, has a phosphatase-like trinuclear zinc center and bound reductants in a zincmediated manner. Disruption of tthb071 increased the sensitivity to oxidative stress. Conclusion: TTHB071 would protect cells from oxidative stress through controlling the metabolism of reductants. Significance: Our findings may explain how cells avoid oxidative damage.

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Section: Resultsmentioning

confidence: 56%

In Vivo, in Vitro, and X-ray Crystallographic Analyses Suggest the Involvement of an Uncharacterized Triose-phosphate Isomerase (TIM) Barrel Protein in Protection against Oxidative Stress

Nakane

Wakamatsu

Masui

et al. 2011

Journal of Biological Chemistry

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“…Most offer a binary response to the ambient environment, with translation either being completely stalled by a stable secondary RNA structure preventing access of ribosomes to the nascent mRNA, or being active when the RNA structure melts, allowing protein synthesis. There are other emerging examples of thermometers which promote translation at lower temperatures in microbes as divergent as Thermus thermophilus (45) and E. coli to mediated cold shock or adaptive responses (46); this post-transcriptional response is mediated by secondary structures in the 5′ UTR of the mRNA which form at lower temperatures to either protect the transcript from degradation, or enable more efficient access of ribosomes to the RBS.…”

Section: Discussionmentioning

confidence: 99%

Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion byNeisseria meningitidis

et al. 2016

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Neisseria meningitidis causes bacterial meningitis and septicemia. It evades the host complement system by upregulating expression of immune evasion factors in response to changes in temperature. RNA thermometers within mRNAs control expression of bacterial immune evasion factors, including CssA, in the 5′-untranslated region of the operon for capsule biosynthesis. We dissect the molecular mechanisms of thermoregulation and report the structure of the CssA thermometer. We show that the RNA thermometer acts as a rheostat, whose stability is optimized to respond in a small temperature range around 37°C as occur within the upper airways during infection. Small increases in temperature gradually open up the structure to allow progressively increased access to the ribosome binding site. Even small changes in stability induced by mutations of imperfect base pairs, as in naturally occurring polymorphisms, shift the thermometer response outside of the desired temperature range, suggesting that its activity could be modulated by pharmacological intervention.

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“…In contrast to the single Tte CspC in T. tengcongensis , T. thermophilus has two Csps: one that mainly contributes to translational control under optimal growth conditions (Csp1) [33], and another that is mainly responsible for the cold shock response (Csp2) [2]. Identifying the genes regulated by Tte CspC and the corresponding mechanisms of regulation will clarify this further.…”

Section: Resultsmentioning

confidence: 99%

“…These include changes in cell wall/membrane composition, protein synthesis rates, energy metabolism, and others [1]. Cold shock responses have been documented in almost all unicellular organisms from thermophiles (such as Thermus thermophilus [2], Thermotoga maritima [3] and Thermus sp. GH5 [4]) to mesophiles (such as Bordetella bronchiseptica [5] and Caulobacter crescentus [6]) to psychrophiles (such as Psychromonas arctica [7] and Pseudoalteromonas haloplanktis [8]).…”

Section: Introductionmentioning

confidence: 99%

“…The number of Eco CspA homologs varies greatly by species. For example, there are nine homologs in E. coli (CspA to CspI) [11]; three in Bacillus subtilis (CspB, CspC, and CspD), with CspB being the major homolog [12]; and two in T. thermophilus HB8, with Csp2 being the major homolog [2]. Cold shock responses of bacteria containing at least two different csp genes have been extensively described; however, studies in bacteria harboring a single csp gene have not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

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RNA-Seq-Based Analysis of Cold Shock Response in Thermoanaerobacter tengcongensis, a Bacterium Harboring a Single Cold Shock Protein Encoding Gene

Liu

Zhang

2014

PLoS ONE

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BackgroundAlthough cold shock responses and the roles of cold shock proteins in microorganisms containing multiple cold shock protein genes have been well characterized, related studies on bacteria possessing a single cold shock protein gene have not been reported. Thermoanaerobacter tengcongensis MB4, a thermophile harboring only one known cold shock protein gene (TtescpC), can survive from 50° to 80°C, but has poor natural competence under cold shock at 50°C. We therefore examined cold shock responses and their effect on natural competence in this bacterium.ResultsThe transcriptomes of T. tengcongensis before and after cold shock were analyzed by RNA-seq and over 1200 differentially expressed genes were successfully identified. These genes were involved in a wide range of biological processes, including modulation of DNA replication, recombination, and repair; energy metabolism; production of cold shock protein; synthesis of branched amino acids and branched-chain fatty acids; and sporulation. RNA-seq analysis also suggested that T. tengcongensis initiates cell wall and membrane remodeling processes, flagellar assembly, and sporulation in response to low temperature. Expression profiles of TtecspC and failed attempts to produce a TtecspC knockout strain confirmed the essential role of TteCspC in the cold shock response, and also suggested a role of this protein in survival at optimum growth temperature. Repression of genes encoding ComEA and ComEC and low energy metabolism levels in cold-shocked cells are the likely basis of poor natural competence at low temperature.ConclusionOur study demonstrated changes in global gene expression under cold shock and identified several candidate genes related to cold shock in T. tengcongensis. At the same time, the relationship between cold shock response and poor natural competence at low temperature was preliminarily elucidated. These findings provide a foundation for future studies on genetic and molecular mechanisms associated with cold shock and acclimation at low temperature.

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Very rapid induction of a cold shock protein by temperature downshift in Thermus thermophilus

Cited by 24 publications

References 33 publications

In Vivo, in Vitro, and X-ray Crystallographic Analyses Suggest the Involvement of an Uncharacterized Triose-phosphate Isomerase (TIM) Barrel Protein in Protection against Oxidative Stress

In Vivo, in Vitro, and X-ray Crystallographic Analyses Suggest the Involvement of an Uncharacterized Triose-phosphate Isomerase (TIM) Barrel Protein in Protection against Oxidative Stress

Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion byNeisseria meningitidis

RNA-Seq-Based Analysis of Cold Shock Response in Thermoanaerobacter tengcongensis, a Bacterium Harboring a Single Cold Shock Protein Encoding Gene

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