Vesicular aptasensor for the detection of thrombin

Müller, Andreas; König, Burkhard

doi:10.1039/c4cc05221h

Cited by 16 publications

(19 citation statements)

References 50 publications

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“…These efforts,with potential sensing applications,t ypically rely on coembedding the receptors and the fluorescent reporters in the vesicle bilayer. [104][105][106] Upon binding,r eceptor sites are spatially reorganized, thus affecting the optical properties of coembedded reporters and triggering ac hange in the vesicle emission properties.T his strategy has been employed to create vesicular aptasensors for the detection of thrombin [106] and adenosine. [107] Molecular recognition and signal-transduction processes at the surface of vesicles have also been harnessed to control enzymatic activity.…”

Section: Membrane Binding and Downstream Eventsmentioning

confidence: 99%

Vesicles in Nature and the Laboratory: Elucidation of Their Biological Properties and Synthesis of Increasingly Complex Synthetic Vesicles

et al. 2017

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The important role of vesicles in many aspects of cell function is well-recognized, but only recently have sophisticated imaging techniques begun to reveal their ubiquity in nature. While we further our understanding of the biological properties of vesicles and their physiological functions, increasingly elegant artificial vesicles are being developed for a wide range of technological applications and basic research. Herein, we examine the state of the art of biological and synthetic vesicles and place their biological features in the context of recent synthetic developments, thus providing a unique overview of these complex and rapidly developing fields. The challenges and opportunities associated with future biological and synthetic studies of vesicles are also presented.

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Section: Membrane Binding and Downstream Eventsmentioning

confidence: 99%

Vesicles in Nature and the Laboratory: Elucidation of Their Biological Properties and Synthesis of Increasingly Complex Synthetic Vesicles

et al. 2017

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“…B. eine Änderung des Fluoreszenzsignals) umzuwandeln. Diese Versuche, die für mögliche Sensoranwendungen von Bedeutung sind, beruhen meist auf der gemeinsamen Einbettung der Rezeptoren und Fluoreszenzsensoren in die Vesikeldoppelschicht . Nach der Bindung werden die Rezeptoren räumlich reorganisiert und beeinflussen so die optischen Eigenschaften der mit ihnen eingebetteten Sensoren, was zu einer Änderung der Emissionseigenschaften des Vesikels führt.…”

Section: Membranbindung Und Nachgelagerte Ereignisseunclassified

“…Diese Strategie wurde z. B. bei der Erzeugung vesikulärer Aptasensoren für den Nachweis von Thrombin und Adenosin verwendet. Die molekularen Erkennungs‐ und Signalübertragungsprozesse an der Vesikeloberfläche wurden auch zur Steuerung enzymatischer Aktivität genutzt …”

Section: Membranbindung Und Nachgelagerte Ereignisseunclassified

Vesikel in der Natur und im Labor: die Aufklärung der biologischen Eigenschaften und die Synthese zunehmend komplexer synthetischer Vesikel

Fernández-Trillo¹,

Grover²,

Stephenson-Brown³

et al. 2017

Angewandte Chemie

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Die wichtige Rolle von Vesikeln in vielen Bereichen der Zellfunktion ist gut bekannt, aber erst seit kurzem offenbart sich mithilfe neuer, komplexerer Bildgebungsverfahren ihre Allgegenwart in der Natur. Während die biologischen Eigenschaften von Vesikeln und ihre physiologischen Funktionen immer besser verstanden werden, werden auch vermehrt sehr elegante, künstliche Vesikel für eine Reihe technischer Anwendungen und die Grundlagenforschung entwickelt. Hier wollen wir den Stand der Technik von biologischen und synthetischen Vesikeln darstellen und ihre biologischen Eigenschaften in Zusammenhang mit aktuellen Syntheseentwicklungen bringen, um so einen Überblick über diese komplexen, rasch aufstrebenden Fachgebiete zu geben. Darüber hinaus werden die Herausforderungen und Möglichkeiten für die zukünftige biologische und präparative Entwicklung von Vesikeln präsentiert.

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“…Until now, probe functionalized aptamers have been selected for targeting various analytes such as cancer cells (Zhang et al ., ), tumor markers (Cha et al ., ) and metal ions (Zhang et al ., ). Although the aptamer, as an effective recognition element, has been investigated extensively in the construction of various biosensors for electrochemical (Miodek et al ., ), photoelectrochemical (Zhao et al ., ), electrochemiluminescent (Li et al ., ), fluorescent (Müller and König, ) and colorimetric analysis (Geng et al ., ), the CE‐based aptamer assay is still in its infancy. On the other hand, the small detection volume in CE often results in poor concentration detection sensitivity; achieving high sensitivity has long been a major goal in CE, especially in the monitoring of rare analytes in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Dual‐signal amplification strategy for ultrasensitive chemiluminescence detection of PDGF–BB in capillary electrophoresis

Cao

Wang

Ren

et al. 2015

Biomedical Chromatography

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Many efforts have been made toward the achievement of high sensitivity in capillary electrophoresis coupled with chemiluminescence detection (CE-CL). This work describes a novel dual-signal amplification strategy for highly specific and ultrasensitive CL detection of human platelet-derived growth factor-BB (PDGF-BB) using both aptamer and horseradish peroxidase (HRP) modified gold nanoparticles (HRP-AuNPs-aptamer) as nanoprobes in CE. Both AuNPs and HRP in the nanoprobes could amplify the CL signals in the luminol-H2 O2 CL system, owing to the excellent catalytic behavior of AuNPs and HRP in the CL system. Meanwhile, the high affinity of aptamer modified on the AuNPs allows detection with high specificity. As proof-of-concept, the proposed method was employed to quantify the concentration of PDGF-BB from 0.50 to 250 fm with a detection limit of 0.21 fm. The applicability of the assay was further demonstrated in the analysis of PDGF-BB in human serum samples with acceptable accuracy and reliability. The result of this study exhibits distinct advantages, such as high sensitivity, good specificity, simplicity, and very small sample consumption. The good performances of the proposed strategy provide a powerful avenue for ultrasensitive detection of rare proteins in biological sample, showing great promise in biochemical analysis.

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Vesicular aptasensor for the detection of thrombin

Abstract: Self-assembled phospholipid vesicles are functionalized with thrombin-binding aptamers using a thiol-click reaction. The resulting aptasensors signal the binding of the analyte to the vesicle surface by changes of the emission properties of membrane co-embedded reporter dyes.

Cited by 16 publications

References 50 publications

Vesicles in Nature and the Laboratory: Elucidation of Their Biological Properties and Synthesis of Increasingly Complex Synthetic Vesicles

Vesicles in Nature and the Laboratory: Elucidation of Their Biological Properties and Synthesis of Increasingly Complex Synthetic Vesicles

Vesikel in der Natur und im Labor: die Aufklärung der biologischen Eigenschaften und die Synthese zunehmend komplexer synthetischer Vesikel

Dual‐signal amplification strategy for ultrasensitive chemiluminescence detection of PDGF–BB in capillary electrophoresis

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