Viabilité des globules rouges humains conservés pendant 35 jours après déplétion en leucocytes (étude in vitro)

Angue, Marion; Chatelain, Pierre; Fiabane, S.; Domy, M.; Guignier, F.; Richaud, Pierre

doi:10.1016/s1140-4639(89)80050-4

Cited by 20 publications

(6 citation statements)

References 16 publications

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“…Prestorage WBC reduction did not significantly affect any of the in vitro storage properties of the RBCCs, although the level of hemolysis at the end of storage was markedly lower in the filtered units when compared to the control group, as also witnessed in other studies [14,[16][17][18]. Therefore our results further confirm that the process of prestorage leukocyte filtration does not reduce the quality of erythrocytes stored over a 49-day period.…”

Section: Discussionsupporting

confidence: 89%

“…As reviewed by Piertersz et al [30], these results favor an early WBC depletion of the blood components before storage. Prestorage filtration can be defined in a number of different ways, for instance in-line [14,15,17,18], sterile dock [16,30], immediately after blood component preparation [14][15][16][17][18][19], 24 h afterwards [30], or any combination of the above. From our results, whole blood filtration prior to component preparation seems to offer another promising alternative.…”

Section: Discussionmentioning

confidence: 99%

“…There is evidence that WBC and platelet fragmentation is rapid during storage and that degradation products such as histamine, elastase, acid phosphatase and serotonin accumulate if WBC and platelets are not removed before storage [11][12][13]. Reports indicate that prestorage WBC depletion of red blood cell concentrates (RBCC) improves the storage characteristics of the units: less hemolysis, lower supernatant potassium, better poststorage 2,3-DPG and ATP values [14][15][16][17]. Recently, in-line filtration has been introduced, which allows early WBC depletion of RBCC in closed systems before storage [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

Rapaille¹,

Moore²,

Flament³

et al. 1997

Vox Sanguinis

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Objectives: Prestorage filtration of blood components appears to be an effective method to reduce leukocyte-induced adverse reactions and other complications. To determine whether it is better to filter whole blood before component separation, we compared the efficiency of in-line filtration of whole blood with that of postseparation filtration. Methods: Blood was collected from normal, healthy donors into either regular triple-bag containers or into whole-blood integral- filter container systems. We then compared the in vitro storage values of leukocyte-depleted red blood cell concentrates (RBCC) kept at 4°C, and plasma frozen for 1 year with nonfiltered blood components as control. Results: All counts of white blood cells after filtration were < 1 X106 per unit. For almost all storage parameters no significant differences were found between leukocyte-reduced RBCC and control units. The plasma fibrinopeptide A values below 30 ng/ ml prior to freezing indicate that filtration does not activate the coagulation factors. Furthermore, the filtration did not influence either the biological values or the coagulation factors of plasma units. Conclusions: Whole blood filtration prior to component preparation seems to offer a useful alternative technique for obtaining leukocyte-reduced RBCC and plasma.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

Rapaille¹,

Moore²,

Flament³

et al. 1997

Vox Sanguinis

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“…Leucocyte depletion decreases the incidence of adverse transfusion outcomes such as HLA alloimmunisation or febrile non-haemolytic reactions [3, 4, 5, 6]. Furthermore, pre-storage leucocyte depletion reduces supernatant levels of leucocyte enzymes [7, 8]and improves biochemical parameters of stored human red blood cells (RBCs) [1, 9, 10, 11, 12]. …”

Section: Introductionmentioning

confidence: 99%

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

et al. 2000

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Background and Objectives: Leucocyte depletion improves the quality of stored blood units. We have studied its role on blood viscosity. Materials and Methods: Viscosity of CPDA-1 blood units was measured in a Couette viscometer at shear rates of 94.5 and 0.1 s^–1 prior to and following filtration with the Leukotrap^® A1 system on day 0 and after 21 days at +4°C. Results: On day 0, high but not low shear viscosity was significantly decreased. The red blood cell morphology was unaffected. On day 21, blood viscosity was increased similarly for unfiltered and filtered samples at both shear rates. The echinocytosis observed after storage correlated with the increase in viscosity. Conclusion: Leucocyte depletion is associated with a decrease in high shear viscosity. This effect is, however, completely lost after 21 days.

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“…Early studies of red cell storage using in-line filtration as an alternative to leucodepleted red cells prior to storage showed that not only could > 1 loglo leucodepletion be consistently obtained, but also that storage-induced potassium leakage and haemolysis could be significantly reduced and the oxygen dissociation characteristics of stored red cells were better maintained (Lovric et al, 1981). Subsequent studies not only confirmed marginally better 2,3diphosphoglycerate (DPG) preservation, but also showed reduced glucose consumption/lactate production during storage, though it was not clear whether this was due to leucocyte metabolism or to a secondary effect of leucocyte enzymes on the membranes of the stored red cells (Angue et al, 1989).…”

mentioning

confidence: 99%

Effects of 3‐5 log₁₀ pre‐storage leucocyte depletion on red cell storage and metabolism

et al. 1994

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A new, in-line high-efficiency 3-5 log10 leucodepletion filter system (Leukotrap RC system) was used to investigate the effect of pre-storage white cell removal on the quality of AS-3 red cell concentrates stored for 42 d at 4 degrees C. Median residual white cell content was 4 x 10(5) when filtration was performed at 22 degrees C within 8 h of phlebotomy (n = 20) and 3.2 x 10(4) when filtration was performed at 4 degrees C 12-24 h after phlebotomy (n = 24). None exceeded 1 x 10(6) WBC per red cell product. Filtration was rapid (median 28 min), and red cell loss averaged (mean +/- 1 SD) 6.4 +/- 0.7%. In a paired study design, post-transfusion recoveries of 42 d stored red cells in the filtered units averaged 84 +/- 6% v 82 +/- 8% for unfiltered units (P < 0.05) and post-storage haemolysis. ATP, osmotic fragility, K+ and pH were significantly (P < 0.05) better in the filtered units. Reduced glycolytic activity was also observed in the filtered units, and there was a correlation between osmotic fragility, glucose consumption, and lactate produced in standard units that was not present in leucodepleted units. In conclusion, this study suggests that leucodepletion of AS-3 red cell concentrates prior to storage results in better maintenance of the integrity of the red cell membrane with reduced glycolytic activity. There was a modest improvement in post-infusion viability sufficient to offset the filtration-induced loss and to result in an equivalent red cell product.

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Viabilité des globules rouges humains conservés pendant 35 jours après déplétion en leucocytes (étude in vitro)

Cited by 20 publications

References 16 publications

Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

Effects of 3‐5 log₁₀ pre‐storage leucocyte depletion on red cell storage and metabolism

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Viabilité des globules rouges humains conservés pendant 35 jours après déplétion en leucocytes (étude in vitro)

Cited by 20 publications

References 16 publications

Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

Effects of 3‐5 log10 pre‐storage leucocyte depletion on red cell storage and metabolism

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Effects of 3‐5 log₁₀ pre‐storage leucocyte depletion on red cell storage and metabolism