Viability and Fertility of Human Hepatic Hydatid Cysts

Manterola, Carlos; Vial, Manuel; Melo, Angélica; Oberg, C; Fonseca, Flery

doi:10.1007/s00268-005-0215-6

Cited by 23 publications

(17 citation statements)

References 18 publications

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“…Todos, pusieron en evidencia falencias metodológi-cas similares que cuestionan la validez interna y externa del estudio, hecho que a su vez hace poner en duda la lógica de la aplicabilidad de este protocolo. Especialmente cuando por otro lado, existe evidencia suficiente que no respalda el uso de este tipo de tratamiento, basado en: la escasa concentración que se obtiene a nivel intraquístico, asociado a la nula diferencia en términos de viabilidad de escólices entre pacientes que reciben este fármaco y un grupo control 8 ; la baja tasa de respuesta que oscila entre un 20% y un 30%; los efectos adversos que incluyen disfunción hepática, pancitopenia e incluso agranulocitosis 7 ; lo que incrementa ostensiblemente los costos con una respuesta final que a la luz de lo analizado es al menos cuestionable.…”

Section: Discussionunclassified

“…Los esquemas terapéuticos utilizados son diversos en dosificación, duración y administración. Se ha utilizado en el pre y postoperatorio, durante días o meses [5][6][7] ; sin embargo, su eficacia y utilidad no gozan aún de sólido soporte científico 8 . Más aún, su efectividad no supera el 30%; su uso se ha asociado a reacciones adversas hasta en el 20% de los casos, y a recurrencia de la enfermedad después de dos años del tratamiento 7,9 .…”

Section: Introductionunclassified

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Uso de Albendazol como tratamiento de la equinococcosis. Toma de decisiones basadas en la validez interna y externa de un estudio concerniente

et al. 2008

Rev Chil Cir

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Section: Discussionunclassified

Section: Introductionunclassified

Uso de Albendazol como tratamiento de la equinococcosis. Toma de decisiones basadas en la validez interna y externa de un estudio concerniente

et al. 2008

Rev Chil Cir

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“…We didn't find any significant relation between viability and cyst size. The difference in viability of protoscoleces can be due to the various factors, including the impact of different situations, such as temperature and humidity, and difference of research methods, for example time of sampling, viability assessment method and genetic variations of Echinococcus granulosus (Manterola et al, 2006;Dicker, Tinar, & Senlik, 2007).…”

Section: Resultsmentioning

confidence: 99%

Fertility of Hydatid Cysts and Viability of Protoscoleces in Slaughtered Animals in Qazvin, Iran

Shahnazi¹,

Jafari²,

Javadi³

et al. 2012

JAS

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This study was conducted to estimate the fertility of hydatid cysts and viability of protoscoleces based on the location, size and type of cysts in slaughtered animals in Qazvin, Iran. Cysts were collected from the livers and lungs of 247 sheep and 275 cattle infected with hydatid cysts. Fertility of the cysts was assessed by examining the cysts' fluid for the presence of protoscoleces and the viability of the protoscoleces was determined using staining with an aqueous solution of 0.1% eosin. The highest and lowest rate of fertility was observed in hepatic cysts of sheep (81.05%), and cattle (1.27%) respectively. Most fertile cysts had medium size (45.03%), while the lowest fertility rate belonged to the small cysts (22.8%) . Relationship between fertility of the cysts and type of infected organs, type of animals and size of cysts were significant (p<0.05). The highest rate of viability was found in cattle's lungs (88.06%) and the lowest rate was seen in sheep's liver cysts (46.49%). There was significant difference in viability of fertile cysts between cattle and sheep organs (p<0.05). Most of sterile, suppurative and calcified cysts were found in cattle's lungs (84.7%), cattle's liver (89.87%) and sheep's liver (6.58%) respectively.In conclusion it can be said that fertility rates of liver and lung hydatid cysts of sheep and viability of their protoscoleces is considerable. In addition, although the fertility of cysts in cattle was low, but they had high viability rate.

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“…However, the metabolites of certain drugs including benzimidazole mebendazole, albendazole and albendazole sulfoxide are potentially toxic in some subjects (Polat et al, 2005;Mandal and Mandal, 2012). Manterola et al (2006) reported that the prevalence of fertile human hydatid cyst was low and fertility is associated with the type of cyst and the presence of biliary communications. The viability assessment in CE is important for in vitro and in vivo studies and also drug experiments.…”

Section: Discussionmentioning

confidence: 99%

Viability loss and ultrastructural changes on protoscolices of human hydatid cysts induced by retinoic acid

Yones¹,

Mahmoud²,

Othman³

et al. 2014

J. Parasitol. Vector Biol.

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Surgical removal of intact hydatid cyst is the most effective treatment for hydatid disease. Recurrence of hydatid cyst is mainly due to dissemination of protoscolices (PSCs) rich fluid during the surgical operation. Therefore, preoperative instillation of a scolicidal agent into the cyst is a common practice with adverse side effects of the used drugs. All-trans retinoic acid (atRA) is the physiological mediator of most of the functions of vitamin A, particularly as a cellular differentiation and apoptosis regulating factor. We hypothesized that instillation of atRA could provide an alternative safe scolicidal approach. We tested the scolicidal effects and ultrastructural changes imposed by atRA on human hydatid cyst PSCs in vitro. Freshly isolated hydatid cyst PSCs were subjected to atRA (at 16.7, 1.67, 0.167 µM and 16.7 nM/L). Changes in protoscolices viability (0.1% eosin exclusion) and morphology (scanning and transmission electron microscopy; SEM and TEM) were investigated. Dose-dependent PSCs death within few minutes to 7 days of exposure to atRA was observed. SEM demonstrated ultrastructural damages including rosteller disorganization, loss of hooks and distortion of hooks morphology. TEM revealed loss of the integrity of the internal tissues of PSCs, an increased vacuolization, formation of large lipid droplets in the distal cytoplasm and aberrant, rounded abnormally large sized mitochondria. atRA is a promising alternative to the available synthetic and chemical scolicidal agents. However, in vivo scolicidal activities of atRA and the possible side effects necessitate further studies.

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Viability and Fertility of Human Hepatic Hydatid Cysts

Abstract: The prevalence of fertility found was low. The main prevalence of fertility was observed in MVCs. Fertility is associated with the type of cyst and the presence of biliary communications.

Cited by 23 publications

References 18 publications

Uso de Albendazol como tratamiento de la equinococcosis. Toma de decisiones basadas en la validez interna y externa de un estudio concerniente

Uso de Albendazol como tratamiento de la equinococcosis. Toma de decisiones basadas en la validez interna y externa de un estudio concerniente

Fertility of Hydatid Cysts and Viability of Protoscoleces in Slaughtered Animals in Qazvin, Iran

Viability loss and ultrastructural changes on protoscolices of human hydatid cysts induced by retinoic acid

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