Viability of Human Blood Leukocytes Compared with Their Respective Cell Lines after Plasma Treatment

Abstract

Non-thermal plasma application has become a promising field of investigation in chronic wound healing research over the past few decades. In addition to its well-characterized antibacterial effects, plasma potentially promotes the growth of eukaryotic cells. To date, mainly epithelial skin cells have been examined regarding the impact of plasma treatment on chronic wound healing. However, immune cells also are involved in wound healing as well as the removal of pathogens. Therefore, we compared the survival behavior of 2 human leukocyte cell lines (a monocyte and a CD4 + T helper cell line) and their respective human blood counterparts after exposure to plasma. Measurements of early and late apoptotic cells demonstrate that freshly isolated blood cells were more susceptible to apoptosis induction than the cell lines. Furthermore, blood and cell line monocytes tolerated longer plasma exposure compared with blood and cell line CD4 + T helper cells.

Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion

“…[ 29 ] While many reports point to a selectivity of gas plasma treatment to induce toxic effects in tumor cells over non‐malignant cells, [ 30–33 ] more comprehensive studies revealed that selectivity depended on the type of tumor cell investigated and the cell line used for comparison. [ 34–37 ] Notwithstanding, increased expression of pro‐immunogenic surface markers in ROS‐treated cells was observed in malignant over non‐malignant cells. [ 38 ]…”

Non-thermal plasma treatment induces MAPK signaling in human monocytes

“…expression, leading to increased survival and apoptosis evasion [32]. Treatment with the apoptosis-inducing agent H 2 O 2 [33] led to an up-regulation of JNK 1/2 to 3.1-and 4.7-(p < 0.05, *) fold of the control as described in the literature [34]. Next to JNK 1/2, p38 MAPK was examined regarding its activation after non-thermal plasma treatment.…”

Redox‐regulation of activator protein 1 family members in blood cancer cell lines exposed to cold physical plasma‐treated medium

“…All experiments were performed with THP‐1 cells, a human monocytic cell line derived from an acute monocytic leukemia patient (CLS, Germany), and Jurkat T cells, an immortalized line of human CD4+ T lymphocyte cells (DSMZ, Germany) as previously described . Briefly, cells were cultivated once a week in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with either 8% (Jurkat) or 10% (THP‐1) fetal bovine serum, 2 mM l ‐glutamine, 0.1 mg/L streptomycin, and 100 U/ml penicillin (all Sigma–Aldrich, Germany) at 37 °C in 95% relative humidity and 5% CO 2 (Binder, Germany).…”