Vibrio azureus sp. nov., a luminous marine bacterium isolated from seawater

Abstract

Two luminous marine bacterial strains, LC2-005 T and LC2-102, were isolated from seawater at Kuroshio Region and Sagami Bay in Japan, respectively. These bacteria were Gram-negative, oxidase-positive, catalase-positive, motile and rod-shaped. On the basis of 16S rRNA gene sequence analysis, strains LC2-005 T and LC2-102 formed a cluster within the Vibrio harveyi species group. However, multilocus sequence analysis using five loci (pyrH, ftsZ, mreB, gyrB and gapA) and DNA-DNA hybridization experiments indicated that these strains were distinct from the currently known Vibrio species. Additionally, these strains differ from related Vibrio species in utilization of glucose, mannitol, inositol, sorbitol, rhamnose, sucrose, melibiose and arabinose, production of lysine decarboxylase, ornithine decarboxylase, tryptophan deaminase, esterase (C4), lipase (C4), chymotrypsin, acid phosphatase, a-glucosidase, b-glucosidase and N-acetyl-bglucosaminidase and the ability to reduce nitrate to nitrite. The major fatty acids were C 15 : 0 iso 2-OH and/or C 16 : 1 v7c, C 16 : 0 , C 18 : 1 v7c and C 14 : 0 . The DNA G+C contents of strains LC2-005 T and LC2-102 were 45.2 and 45.5 mol%, respectively. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains LC2-005 T and LC2-102 belong to the same genospecies and represent a novel species of the genus Vibrio, for which the name Vibrio azureus sp. nov. is proposed. The type strain is LC2-005 T (5NBRC 104587 T 5KCTC 22352 T ).

Vibrio sagamiensis sp. nov., luminous marine bacteria isolated from sea water

“…1 and 2 V. alginolyticus, V. campbellii, V. mytili, V. natriegens, V. parahaemolyticus, V. rotiferianus and V. azureus (Sawabe et al, 2007;Yoshizawa et al, 2009) (Fig. 3 and Fig.…”

Refined identification of Vibrio bacterial flora from Acanthasther planci based on biochemical profiling and analysis of housekeeping genes

“…campbellii, V. rotiferianus, V. alginolyticus, V. parahaemolyticus, V. mytili, V. natriegens, the newly described species V. azureus (Yoshizawa et al 2009), V. owensii (CanoGomez et al 2010), and V. sagamiensis (Yoshizawa et al 2010). Among this clade, the V. harveyi-related species belonging to the so-called V. harveyi group (V. harveyi, V. campbellii, V. rotiferianus, and V. owensii) are phenotypically and genetically indistinguishable, sharing almost identical biochemical profiles and 16S rRNA gene sequences (Gomez-Gil et al 2004, Cano-Gomez et al 2010.…”

Unique and conserved genome regions in Vibrio harveyi and related species in comparison with the shrimp pathogen Vibrio harveyi CAIM 1792

“…Many vibrios have two types of flagellum: one polar flagellum which is produced under liquid conditions and several lateral flagella that are produced in more viscous environments. Within this genus, one of the main groups of species is the Harveyi clade, which contains Vibrio alginolyticus, V. azureus, V. campbellii, V. harveyi, V. jasicida, V. mytili, V. natriegens, V. owensii, V. rotiferianus, V. parahaemolyticus and V. sagamiensis Hoffmann et al, 2013;Sawabe et al, 2007;Yoshizawa et al, 2009). The clade includes important opportunistic pathogens of cultured aquatic organisms and strains of medical relevance (Lin et al, 2010;Thompson et al, 2004).…”