This study explores the potential of online traffic safety education for adolescent motorcyclists in Pakistan. An e-learning platform, “Route 2 School” (R2S), was developed focusing on traffic knowledge, situation awareness, risk detection, and risk management. Male students (14–18 years) who commute to school by motorcycle were divided into an experimental group (EG) and a control group (CG), both completing pre- and post-measurement questionnaires. The EG showed significant improvement in knowledge, risk detection, and risk management compared to the CG, but not in situation awareness. Participants reported increased traffic safety awareness and suggested adding more interactive elements. The R2S platform’s scores revealed better performance in risk detection and risk management modules than situation awareness. Time spent on modules varied, with situation awareness requiring the most time. Adolescents expressed satisfaction with the platform, acknowledging its role in increasing traffic awareness. This study provides initial insights into the effectiveness of online traffic safety education in Pakistan, highlighting the potential to address the lack of comprehensive traffic safety education in schools. Further research and stakeholder engagement are recommended to integrate such platforms into formal education, potentially reducing traffic-related injuries among adolescent motorcyclists in developing countries.