Vierzig Jahre Evolution im Reagenzglas

Joyce, Gerald F.

doi:10.1002/ange.200701369

Cited by 51 publications

(9 citation statements)

References 103 publications

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“…Aptamers are either DNA or RNA single strands that can fold into certain active conformations and bind to many target structures with usually high affinities and good selectivities and can hence be used for example as inhibitors. [23,24] Therefore we got interested in preparing caged aptamers and teamed up with the group of Günter Mayer at the University of Bonn who had been studying aptamers for quite some time. One extensively studied aptamer is the aptamer HD1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Light-Activatable Nucleic Acids 'Caged' at the Nucleobases

Buff¹,

Mack²,

Heckel³

2009

Chimia

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The attachment of photolabile groups to biologically active molecules offers a very versatile way to put a biological effect under the control of an external trigger signal and thus confers spatiotemporal and dose control to this effect. Over the last years we have prepared a number of oligonucleotide derivatives that are modified in such a way. These derivatives are usually referred to as 'caged compounds' and in particular our photolabile 'caging groups' are located at the nucleobases of oligonucleotides so that the Watson-Crick interaction is temporarily impossible. Thus several nucleic acid-based applications have now become controllable with light.

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Section: Resultsmentioning

confidence: 99%

Light-Activatable Nucleic Acids 'Caged' at the Nucleobases

Buff¹,

Mack²,

Heckel³

2009

Chimia

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“…By taking advantage of the fact that formation of the RNA-ligand complex increases the stability of an RNA structure compared to that of the RNA by itself, several gene regulation systems were engineered in which Shine-Dalgarno (SD) and initiation codon sequences were selectively sequestered depending on the presence of an exogenously added ligand, which altered the structure of the mRNA upon binding. [4] Such results strongly suggested that the RNA World could have benefited from feedback regulatory mechanisms to regulate gene expression.…”

Section: Introductionmentioning

confidence: 95%

“…However, it was repeatedly shown by using SELEX (Systematic Evolution of Ligands by EXponential enrichment) that RNA can be a "good contortionist" at building complex architectures. [3] A large number of artificial RNA aptamers have been isolated that respond to various small molecules, such as theophylline, adenosine triphosphate, amino acids and organic dyes (recently reviewed [4] ). By taking advantage of the fact that formation of the RNA-ligand complex increases the stability of an RNA structure compared to that of the RNA by itself, several gene regulation systems were engineered in which Shine-Dalgarno (SD) and initiation codon sequences were selectively sequestered depending on the presence of an exogenously added ligand, which altered the structure of the mRNA upon binding.…”

Section: Introductionmentioning

confidence: 99%

Riboswitches: Ancient and Promising Genetic Regulators

et al. 2009

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Bait and switch: Metabolite‐sensing riboswitches make use of RNA structural modulation to regulate gene expression, as illustrated in the scheme, in response to subtle changes in metabolite concentrations. This review describes the current knowledge about naturally occurring riboswitches and their growing potential as antibacterial cellular targets and as molecular biosensors.magnified imageNewly discovered metabolite‐sensing riboswitches have revealed that cellular processes extensively make use of RNA structural modulation to regulate gene expression in response to subtle changes in metabolite concentrations. Riboswitches are involved at various regulation levels of gene expression, such as transcription attenuation, translation initiation, mRNA splicing and mRNA processing. Riboswitches are found in the three kingdoms of life, and in various cases, are involved in the regulation of essential genes, which makes their regulation an essential part of cell survival. Because riboswitches operate without the assistance of accessory proteins, they are believed to be remnants of an ancient time, when gene regulation was strictly based on RNA, from which are left numerous “living molecular fossils”, as exemplified by ribozymes, and more spectacularly, by the ribosome. Due to their nature, riboswitches hold high expectations for the manipulation of gene expression and the detection of small metabolites, and also offer an unprecedented potential for the discovery of novel classes of antimicrobial agents.

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“…9,10 DNAzymes can be artificially identified from a randomsequence DNA library by in vitro selection [11][12][13][14] and have been increasingly applied as molecular tools for various applications. [15][16][17][18] One powerful type of DNAzyme is an RNAcleaving fluorogenic DNAzyme (RFD).…”

Section: Introductionmentioning

confidence: 99%

A Catalytic DNA Probe with Stem-loop Motif for Human T47D Breast Cancer Cells

et al. 2015

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In vitro selection methods allow for isolation of DNAzymes (catalytic DNAs) from random DNA pools. Here we describe a fluorogenic DNAzyme, LYF5, isolated using a double-random selection approach: a random DNA pool was selected against a complex molecular mixture derived from a breast cancer cell line, T47D. LYF5 specifically indicates the T47D breast cancer cell line with high sensitivity. After sequence optimization, the second-generation DNAzyme, 2G-LYF5, exhibited an approximately 2-fold higher cleavage percentage. Finally, we have determined that the intramolecular stem-loop motif plays a crucial role in 2G-LYF5 activity. Our findings underscore the capability of singlestranded DNA molecules to perform highly sophisticated functions that are amenable to the development of diagnostic tests for early identification of breast cancer.

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Vierzig Jahre Evolution im Reagenzglas

Cited by 51 publications

References 103 publications

Light-Activatable Nucleic Acids 'Caged' at the Nucleobases

Light-Activatable Nucleic Acids 'Caged' at the Nucleobases

Riboswitches: Ancient and Promising Genetic Regulators

A Catalytic DNA Probe with Stem-loop Motif for Human T47D Breast Cancer Cells

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