Violaceols Function as Actin Inhibitors Inducing Cell Shape Elongation in Fibroblast Cells

Asami, Yukihiro; Jang, Jae‐Hyuk; Oh, Hyuncheol; Sohn, Jae Hak; Kim, Jong Won; Moon, Dong Oh; Kwon, Oh‐Seung; Kawatani, Masahito; Osada, Hiroyuki; Kim, Bo Yeon; Ahn, Jong Seog

doi:10.1271/bbb.120074

Cited by 12 publications

(8 citation statements)

References 43 publications

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“…53 β-Actin is the main component of cytoskeletal filaments, playing an important role in cell shape elongation. 54 Our results indicated that the biofunctionalized hydrogel micropatterns not only regulated the cell orientation, but also obviously increased the expression of both BDNF and β-actin inside the cells. The prepared hydrogel was also found to improve the secretion of neurotropic factors, such as GDNF, NGF, and BDNF, which are essential during nerve regeneration.…”

Section: Discussionmentioning

confidence: 54%

“…BDNF promotes the survival of neurons and promotes the growth and differentiation of new neurons and synapses . β-Actin is the main component of cytoskeletal filaments, playing an important role in cell shape elongation . Our results indicated that the biofunctionalized hydrogel micropatterns not only regulated the cell orientation, but also obviously increased the expression of both BDNF and β-actin inside the cells.…”

Section: Discussionmentioning

confidence: 63%

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Construction of Biofunctionalized Anisotropic Hydrogel Micropatterns and Their Effect on Schwann Cell Behavior in Peripheral Nerve Regeneration

Li¹,

Zhang³

et al. 2019

ACS Appl. Mater. Interfaces

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Hydrogels have promising application in tissue regeneration due to their excellent physicochemical and biocompatible properties, whereas anisotropic micropatterns are been proven to directionally induce cell alignment and accelerate cell migration. However, an effect of biofunctionalized anisotropic hydrogel micropatterns on nerve regeneration has rarely been reported. In this study, the anisotropic polyacrylamide (PAM) hydrogel micropatterns with aligned ridge/ groove structures were first prepared via in situ free radical polymerization and micromolding, and then biofunctionalized using YIGSR peptide for better promoting cell growth. The morphology, swelling ratio, wettability, mechanical properties, and stability of the prepared hydrogel were characterized. The successful immobilization of YIGSR peptide on the PAM hydrogel was monitored using FTIR, immunofluorescence staining, and ELISA. The effects on adhesion, directional growth, and biological function of Schwann cells were evaluated. The results displayed that the anisotropic PAM hydrogel micropatterns with inner porous structure possessed good stability, swelling, and mechanical properties. The YIGSR peptide could be well immobilized on hydrogel micropatterns with a percentage of 62.6%. The biofunctionalized anisotropic hydrogel micropatterns could effectively regulate the orientation growth of Schwann cells, and obviously up-regulate BDNF (40%) and β-actin (50%) expression compared with single hydrogel micropatterns, without negatively affecting the normal secretion of neurotropic factors by Schwann cells. To the best of our knowledge, this is the first time to study the construction and effect of biofunctionalized anisotropic hydrogel micropatterns on nerve regeneration, which may provide an experimental and theoretical basis for the design and development of artificial implants for nerve regeneration application.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 63%

Construction of Biofunctionalized Anisotropic Hydrogel Micropatterns and Their Effect on Schwann Cell Behavior in Peripheral Nerve Regeneration

Li¹,

Zhang³

et al. 2019

ACS Appl. Mater. Interfaces

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“…[10,11], Leathesia nana [12], Phyllanthus atropurpureus [13], Symphyocladia latiuscula [14], and so on. Diphenyl ethers exhibit a wide range of interesting biological activities, such as antimicrobial [1,3,6,8,10], cytotoxic [2,6,9], radical-scavenging [11], enzyme inhibitory [5], actin inhibitory [15], anti-HSV-1 [2], and phytocidal activities [16,17]. In early chemical investigation, we reported the isolation of two diphenyl ethers, 2-isopentenyldiorcinol (9) and diorcinol (10), from a strain of endolichenic fungus Aspergillus sp.…”

Section: Introductionmentioning

confidence: 99%

Diphenyl ethers from Aspergillus sp. and their anti-Aβ42 aggregation activities

et al. 2014

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“…It is sometimes difficult to reveal a mode of action directly from an activity-guided investigation, and thus it is necessary to design detailed assay systems [3][4][5]. In an effort to determine the mechanisms of biological activity, we screened these active compounds by using a rat 3Y1 fibroblast cell morphological change assay and were able to identify unique tubulin and actin inhibitors as well as visualize phenotypes, including disrupted cell-cell interaction, cellular elongation, and cellular outgrowth [6]. In the course of this investigation, CRM646-A was identified as a compound that quickly induced nucleus condensation, plasma membrane disruption and morphological changes by increasing intracellular Ca…”

Section: Introductionmentioning

confidence: 99%

CRM646-A, a Fungal Metabolite, Induces Nucleus Condensation by Increasing Ca2+ Levels in Rat 3Y1 Fibroblast Cells

Asami

Kim

Jang

et al. 2020

Journal of Microbiology and Biotechnology

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We previously identified a new heparinase inhibitor fungal metabolite, named CRM646-A, which showed inhibition of heparinase and telomerase activities in an in vitro enzyme assay and antimetastatic activity in a cell-based assay. In this study, we elucidated the mechanism by which CRM646-A rapidly induced nucleus condensation, plasma membrane disruption and morphological changes by increasing intracellular Ca 2 + levels. Furthermore, PD98059, a mitogen-activated protein kinase (MEK) inhibitor, inhibited CRM646-A-induced nucleus condensation through ERK1/2 activation in rat 3Y1 fibroblast cells. We identified CRM646-A as a Ca 2 + ionophore-like agent with a distinctly different chemical structure from that of previously reported Ca 2 + ionophores. These results indicate that CRM646-A has the potential to be used as a new and effective antimetastatic drug.

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Violaceols Function as Actin Inhibitors Inducing Cell Shape Elongation in Fibroblast Cells

Cited by 12 publications

References 43 publications

Construction of Biofunctionalized Anisotropic Hydrogel Micropatterns and Their Effect on Schwann Cell Behavior in Peripheral Nerve Regeneration

Construction of Biofunctionalized Anisotropic Hydrogel Micropatterns and Their Effect on Schwann Cell Behavior in Peripheral Nerve Regeneration

Diphenyl ethers from Aspergillus sp. and their anti-Aβ42 aggregation activities

CRM646-A, a Fungal Metabolite, Induces Nucleus Condensation by Increasing Ca2+ Levels in Rat 3Y1 Fibroblast Cells

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