Viral, Fungal and Bacterial Disease Resistance in Transgenic Plants

Saharan͙, Vinod; Jain, Devendra; Pareek, Sunil; Pal, Ajay; Kumaraswamy, R.V.; Jakhar, Sarita Kumari; Singh, Manvendra

doi:10.1007/978-3-319-22518-0_17

Cited by 14 publications

(12 citation statements)

References 129 publications

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“…Although Bs4C and Xa10 proteins might function via different cell death signalling from the ER, the ER‐localized executor R proteins function broadly across monocotyledonous and dicotyledonous plants, which made it possible for us to engineer AvrXa10/ EBE AvrXa10 ‐dependent and Bs4C‐mediated transgenic resistance in rice. Transgenic resistance has long been used as a method for the control of plant diseases (Cillo and Palukaitis, ; Saharan et al ., ). Successful genetic engineering by the introduction of R genes from unrelated plant species has been reported to generate broad‐spectrum resistant plants (Tai et al ., ; Thilmony et al ., ; Whitham et al ., ; Zhao et al ., ).…”

Section: Discussionmentioning

confidence: 97%

The pepper Bs4C proteins are localized to the endoplasmic reticulum (ER) membrane and confer disease resistance to bacterial blight in transgenic rice

Wang

Zeng

Tian

et al. 2018

Molecular Plant Pathology

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Transcription activator-like effector (TALE)-dependent dominant disease resistance (R) genes in plants, also referred to as executor R genes, are induced on infection by phytopathogenic bacteria of the genus Xanthomonas harbouring the corresponding TALE genes. Unlike the traditional R proteins, the executor R proteins do not determine the resistance specificity and may function broadly in different plant species. The executor R gene Bs4C-R in the resistant genotype PI 235047 of the pepper species Capsicum pubescens (CpBs4C-R) confers disease resistance to Xanthomonas campestris pv. vesicatoria (Xcv) harbouring the TALE genes avrBsP/avrBs4. In this study, the synthetic genes of CpBs4C-R and two other Bs4C-like genes, the susceptible allele in the genotype PI585270 of C. pubescens (CpBs4C-S) and the CaBs4C-R homologue gene in the cultivar 'CM334' of Capsicum annum (CaBs4C), were characterized in tobacco (Nicotiana benthamiana) and rice (Oryza sativa). The Bs4C genes induced cell death in N. benthamiana. The functional Bs4C-eCFP fusion proteins were localized to the endoplasmic reticulum (ER) membrane in the leaf epidermal cells of N. benthamiana. The Xa10 promoter-Bs4C fusion genes in transgenic rice conferred strain-specific disease resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight in rice, and were specifically induced by the Xa10-incompatible Xoo strain PXO99 (pHM1avrXa10). The results indicate that the Bs4C proteins from pepper species function broadly in rice and the Bs4C protein-mediated cell death from the ER is conserved between dicotyledonous and monocotyledonous plants, which can be utilized to engineer novel and enhanced disease resistance in heterologous plants.

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Section: Discussionmentioning

confidence: 97%

The pepper Bs4C proteins are localized to the endoplasmic reticulum (ER) membrane and confer disease resistance to bacterial blight in transgenic rice

Wang

Zeng

Tian

et al. 2018

Molecular Plant Pathology

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“…Prior to GES development, the genetic engineering of virus resistant plants has been mainly undertaken using viral sequences, which are introduced in the genome of the susceptible plant through genetic transformation methods (Saharan et al, 2016). At least 25 viruses have been used to develop virus resistant plants by inserting the viral sequence itself (Wani and Sanghera, 2010; Saharan et al, 2016).…”

Section: Mechanisms Of Genome Editng Systemsmentioning

confidence: 99%

“…At least 25 viruses have been used to develop virus resistant plants by inserting the viral sequence itself (Wani and Sanghera, 2010; Saharan et al, 2016). Although several techniques for genetic transformation of plants are available, the most popular techniques are Agrobacterium infection and ballistic bombardment (Ye, 2015).…”

Section: Mechanisms Of Genome Editng Systemsmentioning

confidence: 99%

“…Both methods have been used to develop antiviral strategies focused on PDR approach. When the complete sequence of a virus gene is inserted into the host plant genome, to interfere with the life cycle of the target virus, the PDR approach is referred as viral protein mediated resistance (Saharan et al, 2016). Within such approach the major viral proteins used are the coat protein, replicase protein, movement protein, and replication-associated protein (Prins et al, 2008).…”

Section: Mechanisms Of Genome Editng Systemsmentioning

confidence: 99%

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Antiviral Defenses in Plants through Genome Editing

Romay

Bragard

2017

Front. Microbiol.

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Plant–virus interactions based-studies have contributed to increase our understanding on plant resistance mechanisms, providing new tools for crop improvement. In the last two decades, RNA interference, a post-transcriptional gene silencing approach, has been used to induce antiviral defenses in plants with the help of genetic engineering technologies. More recently, the new genome editing systems (GES) are revolutionizing the scope of tools available to confer virus resistance in plants. The most explored GES are zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats/Cas9 endonuclease. GES are engineered to target and introduce mutations, which can be deleterious, via double-strand breaks at specific DNA sequences by the error-prone non-homologous recombination end-joining pathway. Although GES have been engineered to target DNA, recent discoveries of GES targeting ssRNA molecules, including virus genomes, pave the way for further studies programming plant defense against RNA viruses. Most of plant virus species have an RNA genome and at least 784 species have positive ssRNA. Here, we provide a summary of the latest progress in plant antiviral defenses mediated by GES. In addition, we also discuss briefly the GES perspectives in light of the rebooted debate on genetic modified organisms (GMOs) and the current regulatory frame for agricultural products involving the use of such engineering technologies.

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“…HIGS of Verticillium dahliae VdH1 in transgenic cotton plants conferred effective resistance to the wilt disease pathogen V. dahliae [13], and spraying of long dsRNAs targeting three fungal cytochrome P450 lanosterol C-14α-demethylases significantly improved barley resistance to necrotrophic fungus Fusarium graminearum [14]. By studying plant resistance to pathogens, transgenesis by overexpressing disease resistance genes (R genes) or positive regulators of plant defense and silencing or genome editing of disease susceptibility genes (S genes) has been used to control different plant diseases caused by fungi, bacteria, and viruses [15,16]. The revolutionary biotechnology known as genome editing will probably become a standard tool in plant breeding of disease resistant crops in the future [17].…”

Section: Introductionmentioning

confidence: 99%

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

et al. 2020

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As the major postharvest disease of citrus fruit, postharvest green mold is caused by the necrotrophic fungus Penicillium digitatum (Pd), which leads to huge economic losses worldwide. Fungicides are still the main method currently used to control postharvest green mold in citrus fruit storage. Investigating molecular mechanisms of plant–pathogen interactions, including pathogenicity and plant resistance, is crucial for developing novel and safer strategies for effectively controlling plant diseases. Despite fruit–pathogen interactions remaining relatively unexplored compared with well-studied leaf–pathogen interactions, progress has occurred in the citrus fruit–Pd interaction in recent years, mainly due to their genome sequencing and establishment or optimization of their genetic transformation systems. Recent advances in Pd pathogenicity on citrus fruit and fruit resistance against Pd infection are summarized in this review.

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Viral, Fungal and Bacterial Disease Resistance in Transgenic Plants

Cited by 14 publications

References 129 publications

The pepper Bs4C proteins are localized to the endoplasmic reticulum (ER) membrane and confer disease resistance to bacterial blight in transgenic rice

The pepper Bs4C proteins are localized to the endoplasmic reticulum (ER) membrane and confer disease resistance to bacterial blight in transgenic rice

Antiviral Defenses in Plants through Genome Editing

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

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