Viral glycoproteins: biological role and application in diagnosis

Banerjee, Nilotpal; Mukhopadhyay, Sumi

doi:10.1007/s13337-015-0293-5

Cited by 83 publications

(65 citation statements)

References 87 publications

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“…One of the major inducers of the host immune response is a structural protein that is embedded in the virus membrane known as Spike (S) glycoprotein [15,16]. The glycosylated S protein facilitates the entry of the virus into the host cell [17,18]. The S protein binds to the host cell membrane receptor, Angiotensin-Converting Enzyme-2 (ACE-2), and further its invasion and fusion inside the host cell membrane is mediated by host Transmembrane Serine Protease (TMPRSS2) [19,20].…”

Section: Introductionmentioning

confidence: 99%

In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19

Kumar

Jena

2020

Preprint

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The recent outbreak of the novel coronavirus (SARS-CoV-2) in the Wuhan province of China has taken millions of lives worldwide. In this pandemic situation and absence of known drugs and vaccines against novel coronavirus disease (COVID-19), there is an urgent need for the repurposing of the existing drugs against it. So, here we have examined a safe and cheap alternative against this virus by screening hundreds of nutraceuticals compounds against known therapeutic targets of SARS-COV-2 by molecular docking .The virtual screening results were then analyzed for binding energy and interactive residues in the best binding pose. All these analyses of this study strongly predicted the potential of Folic acid and its derivates like Tetrahydrofolic acid and 5-methyl tetrahydrofolic acid against SARS-COV-2. The strong and stable binding affinity of this water-soluble vitamin and its derivatives against the SARS-COV-2, indicating that they could be valuable drugs against the management of this COVID-19 pandemic. This study could serve as the starting point for further investigation of these molecules through in-vitro and in-vivo assays.

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Section: Introductionmentioning

confidence: 99%

In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19

Kumar

Jena

2020

Preprint

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“…During viral evolution, glycosylation sites are often added and deleted. With this diversity of modification, the complexity of viral glycoproteins that exists in an individual host or host population is increased [10]. Alteration of glycosylation site(s) can have dramatic impacts on virus survival and transmissibility [11,12].…”

Section: Introductionmentioning

confidence: 99%

Glycosylation of viral surface proteins probed by mass spectrometry

Hargett

Renfrow

2019

Current Opinion in Virology

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Viral Fusion Protein Structures and N-glycosylation Sites (NGS). Viral fusion proteins are typically trimers of heterodimers with a globular head domain and a stalk/transmembrane domain. A GlucNAc 1 or GlucNac 2 are modeled at each NGS (Blue). (a) Lassa virus (LASV) glycoprotein (GP) complex contains 11 (NGS) with 7 in GP1 and 4 in GP2 (PDB: 5VK2) [27]. (b) Human immunodeficiency virus (HIV-1) Envelope (Env) GP contains around 29 NGS with 25 in gp120 the outer Env domain and 4 in gp41 the transmembrane domain (PDB: 5FYK) [49 ]. (c) Ebola virus (EBOV) GP has 17 NGS with 15 on GP1 and 2 on GP2 (PDB: 6G9B) [101]. (d) Coronavirus (CoV) spike GP has 26 NGS with 15 in subunit 1 and 11 in subunit 2 (PDB: 6BFU) [89]. (e) Influenza virus hemagglutinin is a homotrimer with 11 NGS (PDB: 4FNK) [92].

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“…Influenza viruses belong to Orthomyxoviridae type A and type B, which cause diseases in humans. Both types of viruses are responsible for the seasonal influenza epidemics; nevertheless, type B causes milder symptoms and is less frequent (2). The predominant hosts for influenza type B viruses are humans, whereas the primary source for influenza type A viruses are birds.…”

Section: Introductionmentioning

confidence: 99%

Design of a Universal Influenza A Vaccine Candidate Based on M2e.FliC; Immunoinformatics Analysis, Protein Modeling, and Its Expression in Escherichia coli

Kolour

Behzadian

Farahmand

et al. 2018

Jundishapur J Microbiol

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Background: Due to the rapid accumulation of mutations in influenza virus and the unpredictability of new influenza, the current influenza vaccines require an almost yearly reformulation. The extracellular domain of matrix protein 2 (M2e) of influenza A viruses is conserved and is an attractive alternative approach to be used as a vaccine with a broad cross-protection. Objectives: In this study, a vector containing three repeats of M2e gene of influenza A virus fused with molecular adjuvant of FliC was constructed. Methods: In silico analysis of 3M2e.FliC chimeric polypeptide was performed based on 3M2e.FliC sequence, virtual fusion construction translation, linear epitope prediction of 3M2e.FliC, 3M2e.FliC modeling, and validation score consideration through immunoinformatics approaches. Expression of 3M2e.FliC was carried out in two strains of Escherichia coli (BL21 [DE3] and ER2566). The fidelity of expression in both hosts was analyzed through a time course of sampling by SDS-PAGE and confirmed by western blotting. Results: The immunoinformatics results indicated that M2e and FliC epitopes were at the surface of protein, which would be accessible for the immune system. The expression results demonstrated that the 3M2e.FliC construct was expressed well in both strains of E. coli, although the efficiency of expression in ER2566 strain was higher than that of BL21 (DE3) strain. Conclusions: The 3M2e.Flic protein as a recombinant antigen may be considered as a universal influenza vaccine candidate after its evaluation and assessment in animal models.

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Viral glycoproteins: biological role and application in diagnosis

Cited by 83 publications

References 87 publications

In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19

In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against COVID-19

Glycosylation of viral surface proteins probed by mass spectrometry

Design of a Universal Influenza A Vaccine Candidate Based on M2e.FliC; Immunoinformatics Analysis, Protein Modeling, and Its Expression in Escherichia coli

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