Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage

Barrios, Melina Elizabeth; Fernández, María Dolores Blanco; Cammarata, Robertina Viviana; Torres, Carolina; Mbayed, Viviana Andrea

doi:10.1016/j.jviromet.2018.10.002

Cited by 24 publications

(14 citation statements)

References 45 publications

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“…Recoveries of spiked whole process control ranged 3.6-40.9% (Lab1) and 3.2-66.6% (Lab2) (Table 4) suggesting that the method was suitable for viral concentration (Haramoto et al 2018;ISO 15216-1:2017). Overall, previous studies showed similar wide recovery ranges in surface and waste waters by using different viruses as process control such as MgV (Farkas et al 2018;Miura et al 2016), PP7 bacteriophage, or AdV (Kundu et al 2013;Prevost et al 2015;Barrios et al 2018). Average recovery percentages ranging from 38 to 49% were obtained in different studies by using adsorption-elution on electro-charged (either positive or negative) filters and summarized in Cashdollar and Wymer (2013)'s review.…”

Section: Interlaboratory Study For the Detection And Quantification Of Potentially Infectious Enteric Viruses In Influent And Effluent Wamentioning

confidence: 82%

Interlaboratory Comparative Study to Detect Potentially Infectious Human Enteric Viruses in Influent and Effluent Waters

Randazzo

Piqueras²,

Evtoski

et al. 2019

Food Environ Virol

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Wastewater represents the main reusable water source after being adequately sanitized by wastewater treatment plants (WWTP). In this sense, only bacterial quality indicators are usually checked to this end, and human pathogenic viruses usually escape from both sanitization procedures and controls, posing a health risk on the use of effluent waters.In this study, we evaluated a protocol based on aluminium adsorption-precipitation to concentrate several human enteric viruses, including norovirus genogroup I (NoV GI), NoV GII, hepatitis A virus (HAV), astrovirus (HAstV), and rotavirus (RV), with limits of detection of 4.08, 4.64, 5.46 log genomic copies/L, 3.31, and 5.41 log PCR units (PCRU)/L, respectively. Furthermore, the method was applied in two independent laboratories to monitor the presence of NoV GI, NoV GII, and HAV in effluent and influent waters collected from five WWTPs at two different sampling dates.Concomitantly, a viability PMAxx-RT-qPCR was applied to all the samples to get information on the potential infectivity of both influent and effluent waters. The range of the titers in influent waters for NoV GI, NoV GII, RV and HAstV was 4.80-7.56, 5.19-7.31 log genomic copies/L, 5.41-6.52, and 4.59-7.33 log PCRU/L, respectively. In effluent waters, the titers ranged between 4. 08-6.27, 4.64-6.08 log genomic copies/L, <5.51, and 3.31-5.58 log PCRU/L. Moreover, the viral titers detected by viability RT-qPCR showed statistical differences with RT-qPCR alone, suggesting the potential viral infectivity of the samples despite some observed reductions. The proposed method could be applied in ill-equipped laboratories, due to the lack of a requirement for a specific apparatus (i.e., ultracentrifuge).

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Section: Interlaboratory Study For the Detection And Quantification Of Potentially Infectious Enteric Viruses In Influent And Effluent Wamentioning

confidence: 82%

Interlaboratory Comparative Study to Detect Potentially Infectious Human Enteric Viruses in Influent and Effluent Waters

Randazzo

Piqueras²,

Evtoski

et al. 2019

Food Environ Virol

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“…dPCR quantifies the viral genome and its population count in wastewaters without depending on known and calibrated standards [134] , [135] . RT-PCR, RT-qPCR, and nested PCR use specific primers and probes for detection and quantification [136] . The detection rates depend on the primer and probes used to detect target virus which should be revised frequently to assure the novel strain detection [77] .…”

Section: Detection Of Virus In Environmental Matricesmentioning

confidence: 99%

SARS-CoV-2 in environmental perspective: Occurrence, persistence, surveillance, inactivation and challenges

Mohan

Hemalatha

Kopperi

et al. 2021

Chemical Engineering Journal

125

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“…Human JC (JCPyV) and BK (BKPyV) polyomaviruses were quantitively detected by qPCR using the oligonucleotides and reaction conditions described by Barrios et al (2018). On the other hand, a fragment of the gene encoding for the human Ribonuclease P (RNase P) was quantitively detected by qPCR using the system described by the US Centers for Disease Control and Prevention (CDC, 2020).…”

Section: Normalization With Human Polyomaviruses (Hpyv) and Ribonuclease P (Rnase P) As Human Input Indicatorsmentioning

confidence: 99%

“…Table 1. Oligonucleotides: forward (F) and reverse (R) primers and Taq-Man® probes (P) used for the detection of SARS-CoV-2 N gene (CDC, 2020), HPyV (Barrios et al, 2018) and RNase P gene (CDC, 2020). All the extracted nucleic acids from the concentrated water samples were analyzed for HPyV and RNaseP, by duplicate, using positive (plasmid) and negative (ultrapure water) controls.…”

Section: Normalization With Human Polyomaviruses (Hpyv) and Ribonuclease P (Rnase P) As Human Input Indicatorsmentioning

confidence: 99%

Tracking SARS-CoV-2 in rivers as a tool for epidemiological surveillance

Maidana-Kulesza

Poma

Sanguino-Jorquera

et al. 2021

Preprint

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The aim of this work was to evaluate if rivers could be used for SARS-CoV-2 surveillance to support health authorities. Five sampling points from three rivers (AR-1 and AR-2 in Arenales River, MR-1 and MR-2 in Mojotoro River, and CR in La Caldera River) from the Province of Salta (Argentina), two of them receiving the discharges of the wastewater plants (WWTP) of the city of Salta, were monitored from July to December 2020 during the first wave of COVID-19. Fifteen water samples from each point (75 samples in total) were collected and characterized physico-chemically and microbiologically and SARS-CoV-2 was quantitatively detected by RT-qPCR. In addition, two targets linked to human contributions, human polyomavirus (HPyV) and RNase P, were quantified and used to normalize the SARS-CoV-2 concentration, which was ultimately compared to the active reported COVID-19 cases. Statistical analyses allowed us to verify the correlation between SARS-CoV-2 and the concentration of fecal indicator bacteria (FIB), as well as to find similarities and differences between sampling points. La Caldera River showed the best water quality, and FIB were within acceptable limits for recreational activities. Although Mojotoro River receives the discharge of the northern WWTP of the city, it did not affect the water quality. Instead, the Arenales River presented the poorest water quality and the river at AR-2 was negatively affected by the discharges of the southern WWTP, which contributed to the significant increase of fecal contamination. SARS-CoV-2 was only found in about half of the samples and in low concentrations in La Caldera and Mojotoro Rivers, while it was high and persistent in the Arenales River. None of the two human tracers was detected in CR, only HPyV was found in MR-1, MR-2 and AR-1, and both were quantified in AR-2. The experimental and the normalized (using the two tracers) viral concentrations strongly correlated with the curve of active reported COVID-19 cases; thus, the Arenales River at AR-2 reflected the epidemiological situation of the city. This is, to the best of our knowledge, the first study that showed the dynamic of SARS-CoV-2 concentration in an urban river highly impacted by wastewater and proved that can be used for SARS-CoV-2 surveillance to support health authorities.

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Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage

Cited by 24 publications

References 45 publications

Interlaboratory Comparative Study to Detect Potentially Infectious Human Enteric Viruses in Influent and Effluent Waters

Interlaboratory Comparative Study to Detect Potentially Infectious Human Enteric Viruses in Influent and Effluent Waters

SARS-CoV-2 in environmental perspective: Occurrence, persistence, surveillance, inactivation and challenges

Tracking SARS-CoV-2 in rivers as a tool for epidemiological surveillance

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