Virgibacillus byunsanensis sp. nov., isolated from a marine solar saltern

Abstract

A Gram-variable, motile, endospore-forming and rod-shaped bacterial strain, ISL-24T, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by a polyphasic study. Strain ISL-24T grew optimally at pH 7.0–8.0, at 30–37 °C and in the presence of 8 % (w/v) NaCl. It contained MK-7 as the predominant menaquinone and anteiso-C15 : 0 as the predominant fatty acid. The DNA G+C content was 37.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-24T fell within the genus Virgibacillus, clustering with Virgibacillus carmonensis LMG 20964T and Virgibacillus necropolis LMG 19488T, with a bootstrap resampling value of 92.3 %, and exhibiting 97.3 and 97.4 % 16S rRNA gene sequence similarity, respectively, to these strains. Strain ISL-24T exhibited 94.8–96.8 % 16S rRNA gene sequence similarity to the type strains of the other Virgibacillus species. Mean DNA–DNA relatedness values between strain ISL-24T and V. carmonensis DSM 14868T and V. necropolis DSM 14866T were 11 and 19 %, respectively. Differential phenotypic properties of strain ISL-24T, together with the phylogenetic and genetic distinctiveness, revealed that this strain is different from recognized Virgibacillus species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-24T represents a novel species of the genus Virgibacillus, for which the name Virgibacillus byunsanensis sp. nov. is proposed. The type strain is ISL-24T (=KCTC 13259T =CCUG 56754T).

Noncontiguous finished genome sequence and description of Virgibacillus massiliensis sp. nov., a moderately halophilic bacterium isolated from human gut

“…in 1998 and currently consists of mainly Gram-positive, motile, spore-forming, rod-shaped bacteria that are moderately halophilic [11] . Members of the genus Virgibacillus are found in various environments including sediment of a saline lake [12] , [13] , [14] , [15] , traditional salt-fermented seafood [16] , a permafrost core collected from the Canadian high Arctic [17] , a marine solar saltern [18] , [19] , [20] , [21] , biofilm formation on mural paintings [22] , seawater [23] , [24] , field soil, a dairy product sample [25] , a saline mud sample [26] , residual wash water produced during the processing of Spanish-style green table olive sewage [27] , salt crust [28] and fermented fish [29] .…”

Virgibacillus senegalensis sp. nov., a new moderately halophilic bacterium isolated from human gut

“…These bacteria are positive, Gram-variable rods which are ellipsoidal to oval endospores and have DNA G + C content ranging from 36% to 43% [20] . These species were isolated from sediments of a salt lake [20] , [21] , [22] , [23] , fermented seafood in traditional salt [24] , a permafrost core collected from the Canadian high Arctic [25] , a navy solar salt marsh [26] , [27] , soil [28] , seawater [29] , field soil, a dairy product [30] , residual wash water produced during processing wastewater, Spanish-style green table olives [31] , saline sample of mud, salt crust [32] and Thai fermented fish [33] . Here we present a brief classification and a set of features for strain SK-1T (= CSUR P1101 = DSM 28585), with a description of the complete genome sequence and annotation.…”

Biodeterioration Risk Threatens the 3100 Year Old Staircase of Hallstatt (Austria): Possible Involvement of Halophilic Microorganisms

“…The DNA profiles obtained allow discrimination at the subspecies level, based on the DNA diversity in the entire bacterial genome, offering a broad spectrum of genetic variation [ 17 , 40 ]. Sequencing results showed that most of the isolated strains were moderately halophilic bacteria growing optimally in media containing 3–10% (w/v) salt, as the species of Idiomarina , Psychrobacter [ 41 ], Kocuria [ 42 ] Staphylococcus , Virgibacillus [ 43 ] Planococcus [ 44 ] and Janibacter [ 45 ], and some species of the genera Bacillus . Interestingly, some strains were isolated only in media containing the highest NaCl concentrations (10–20% (w/v) NaCl), as those related to species of Halovibrio , Salicola , Marinococcus and some species of Bacillus [ 46 ].…”