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Halobacillus campisalis sp. nov., containing mesodiaminopimelic acid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus T was found to have cell-wall peptidoglycan based on meso-diaminopimelic acid instead of L-ornithine, as found in other members of the genus Halobacillus. Accordingly, the aim of the present study was to determine the exact taxonomic position of strain ASL-17T by a polyphasic characterization that included determination of the phenotypic and chemotaxonomic properties, detailed phylogenetic analysis based on 16S rRNA gene sequences and genetic analysis. Strain ASL-17T was isolated from a sediment collected from a marine solar saltern of the Yellow Sea, Korea, by means of the dilution-plating technique at 30 u C on marine agar 2216 (MA; Difco) supplemented with 8 % (w/v) NaCl. The type strains of nine Halobacillus species were used as reference strains for DNA-DNA hybridization: H. T and H. yeomjeoni MSS-402 T were obtained in our laboratory (Yoon et al., 2003(Yoon et al., , 2004(Yoon et al., , 2005; and H. aidingensis JCM 12771T and H. dabanensis JCM 12772T were obtained from the Japan Collection of Microorganisms (JCM), Saitama, Japan.The morphological, physiological and biochemical characteristics of strain ASL-17 T were investigated using routine cultivation on MA supplemented with 6 % (w/v) NaCl at 37 u C. Cell morphology was examined by light microscopy (Nikon E600) and transmission electron microscopy. Flagellation was determined by using a Philips CM-20 transmission electron microscope with cells from exponentially growing cultures: for this purpose, the cells were negatively stained with 1 % (w/v) phosphotungstic acid and the grids were examined after being air-dried. Growth under anaerobic conditions wasThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain T is EF486356.

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A Gram-negative, non-motile and pleomorphic bacterial strain, SMK-114 T , which belongs to the class Alphaproteobacteria, was isolated from a tidal flat sample collected in Byunsan, Korea. Strain SMK-114 T grew optimally at pH 7.0-8.0 and 25-30 6C and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain SMK-114 T formed a cluster with Octadecabacter species, with which it exhibited 16S rRNA gene sequence similarity values of 95.2-95.4 %. This cluster was part of the clade comprising Thalassobius species with a bootstrap resampling value of 76.3 %. Strain SMK-114 T exhibited 16S rRNA gene sequence similarity values of 95.1-96.3 % to members of the genus Thalassobius. It contained Q-10 as the predominant ubiquinone and C 18 : 1 v7c as the major fatty acid. The DNA G+C content was 60.0 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain SMK-114 T is considered to represent a novel species in a new genus for which the name Gaetbulicola byunsanensis gen. nov., sp. nov. is proposed. The type strain of Gaetbulicola byunsanensis is SMK-114 T (5KCTC 22632 T 5CCUG 57612 T ).

A Gram-staining-negative, non-spore-forming, facultatively aerobic, non-motile, rod-shaped bacterial strain, BR-3 T , was isolated from a tidal flat on the western coast of Korea, and subjected to a polyphasic study. Strain BR-3 T grew optimally at 25 6C, at pH 6.5-7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BR-3 T fell within the clade comprising species of the genus Mucilaginibacter, joining the type strain of Mucilaginibacter rigui, with which it exhibited highest 16S rRNA gene sequence similarity (98.2 %). 16S rRNA gene sequence similarity values between strain BR-3 T and the type strains of the other species of the genus Mucilaginibacter were in the range 93.8-95.9 %. A mean DNA-DNA relatedness value between strain BR-3 T and M. rigui KCTC 12534 T was 21 %. Strain BR-3 T contained MK-7 as the predominant menaquinone and C 16 : 1 v7c and/or iso-C 15 : 0 2-OH and iso-C 15 : 0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and an unidentified aminophospholipid. The DNA G+C content was 49.8 mol%. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain BR-3 T demonstrated that this strain is separate from M. rigui as well as the other species of the genus Mucilaginibacter. On the basis of the data presented, strain BR-3 T is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter lutimaris sp. nov. is proposed. The type strain is BR-3 T (5KCTC 23461 T 5CCUG 60742 T ).The genus Mucilaginibacter was first proposed by Pankratov et al. (2007) with the descriptions of two novel species, Mucilaginibacter paludis and Mucilaginibacter gracilis, and phylogenetically belongs to the phylum Bacteroidetes. Männistö et al., 2010). Members of the genus Mucilaginibacter have been isolated from peat bogs, soils, dried rice straw and wetland freshwater (Pankratov et al., 2007;Urai et al., 2008;An et al., 2009;Baik et al., 2010;Madhaiyan et al., 2010). In this study, a Mucilaginibacter-like bacterial strain, designated BR-3 T , isolated from a tidal flat sediment on the western coast of the Korean peninsula, is described. The aim of the present work was to determine the exact taxonomic position of strain BR-3 T by using a polyphasic characterization that included the determination of phenotypic properties, a detailed phylogenetic investigation based on 16S rRNA gene sequences and genetic analysis.Strain BR-3 T was isolated by the dilution plating technique on R2A agar (Difco) at 25 u C. M. rigui KCTC 12534 T , which was used as a reference strain for phenotypic characterization, fatty acid and polar lipid analyses and DNA-DNA hybridization, was obtained from the Korean Collection for Type Cultures (KCTC), Taejon, Republic of Korea. M. paludis (type species of the genus Mucilaginibacter) KACC 13450 T , which was used as a reference strain for fatty acid and polar lipid analyses, was obtained from the Korean Agricultural Culture Collection (KACC), Suwon...