Mucilaginibacter lutimaris sp. nov., isolated from a tidal flat sediment

Abstract

A Gram-staining-negative, non-spore-forming, facultatively aerobic, non-motile, rod-shaped bacterial strain, BR-3 T , was isolated from a tidal flat on the western coast of Korea, and subjected to a polyphasic study. Strain BR-3 T grew optimally at 25 6C, at pH 6.5-7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BR-3 T fell within the clade comprising species of the genus Mucilaginibacter, joining the type strain of Mucilaginibacter rigui, with which it exhibited highest 16S rRNA gene sequence similarity (98.2 %). 16S rRNA gene sequence similarity values between strain BR-3 T and the type strains of the other species of the genus Mucilaginibacter were in the range 93.8-95.9 %. A mean DNA-DNA relatedness value between strain BR-3 T and M. rigui KCTC 12534 T was 21 %. Strain BR-3 T contained MK-7 as the predominant menaquinone and C 16 : 1 v7c and/or iso-C 15 : 0 2-OH and iso-C 15 : 0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and an unidentified aminophospholipid. The DNA G+C content was 49.8 mol%. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain BR-3 T demonstrated that this strain is separate from M. rigui as well as the other species of the genus Mucilaginibacter. On the basis of the data presented, strain BR-3 T is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter lutimaris sp. nov. is proposed. The type strain is BR-3 T (5KCTC 23461 T 5CCUG 60742 T ).The genus Mucilaginibacter was first proposed by Pankratov et al. (2007) with the descriptions of two novel species, Mucilaginibacter paludis and Mucilaginibacter gracilis, and phylogenetically belongs to the phylum Bacteroidetes. Männistö et al., 2010). Members of the genus Mucilaginibacter have been isolated from peat bogs, soils, dried rice straw and wetland freshwater (Pankratov et al., 2007;Urai et al., 2008;An et al., 2009;Baik et al., 2010;Madhaiyan et al., 2010). In this study, a Mucilaginibacter-like bacterial strain, designated BR-3 T , isolated from a tidal flat sediment on the western coast of the Korean peninsula, is described. The aim of the present work was to determine the exact taxonomic position of strain BR-3 T by using a polyphasic characterization that included the determination of phenotypic properties, a detailed phylogenetic investigation based on 16S rRNA gene sequences and genetic analysis.Strain BR-3 T was isolated by the dilution plating technique on R2A agar (Difco) at 25 u C. M. rigui KCTC 12534 T , which was used as a reference strain for phenotypic characterization, fatty acid and polar lipid analyses and DNA-DNA hybridization, was obtained from the Korean Collection for Type Cultures (KCTC), Taejon, Republic of Korea. M. paludis (type species of the genus Mucilaginibacter) KACC 13450 T , which was used as a reference strain for fatty acid and polar lipid analyses, was obtained from the Korean Agricultural Culture Collection (KACC), Suwon...

Mucilaginibacter ginsengisoli sp. nov., isolated from a ginseng-cultivated soil

“…Strain B4Y-8 T contained phosphatidylethanolamine and an unidentified aminophospholipid as the major polar lipids (Fig. S2), which was also shown for M. litoreus (Yoon et al , 2012), M. lutimaris (Kim et al , 2012b), M. rigui (Kim et al , 2012b) and M. defluvii (Hwang et al , 2014). Strain B4Y-8 T also contained an unidentified aminolipid, unidentified phospholipids and unidentified polar lipids.…”

“…The genus Mucilaginibacter is a member of the family Sphingobacteriaceae , in the phylum Bacteroidetes . Strains of the genus Mucilaginibacter are Gram-negative, non-motile rods that produce a large amount of extracellular polymeric substance (EPS); they are strictly aerobic or facultatively anaerobic chemo-organotrophs; they have menaquinone 7 (MK-7) as the predominant quinone although some species contain MK-6 as well; and the G+C content of the DNA varies between 39.1 and 49.8 mol% (Baik et al , 2010; Chen et al , 2014; Kim et al , 2012b; Pankratov et al , 2007; Urai et al , 2008).…”

“…At the time of this writing, the genus comprised 33 recognized species, which were isolated from soil (Jiang et al , 2012; Kang et al , 2011; Luo et al , 2009; Urai et al , 2008), rhizosphere (Kim et al , 2010, 2012a; Madhaiyan et al , 2010), live or dead plants (Kämpfer et al , 2014; Khan et al , 2013), wetlands such as a rice paddy (Jeon et al , 2009), and peat bog (Pankratov et al , 2007), freshwater (Joung et al , 2014) and marine environments (Kim et al , 2012b; Yoon et al , 2012).…”

Mucilaginibacter aquaedulcis sp. nov., isolated from fresh water

“…Strains of Mucilaginibacter may contain iso-C 15 : 0 as the major lipid and also contain monounsaturated fatty acids (summed feature 3, comprising C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) as another major fatty acid component. Species of the genus Mucilaginibacter have been isolated from various environmental sources including soil (Jiang et al , 2012; Kang et al , 2011), dried rice straw(An et al , 2009), fresh water (Baik et al , 2010; Joung & Joh, 2011), rhizosphere (Han et al , 2012; Kim et al , 2012a) and tidal flat sediment (Yoon et al , 2012; Kim et al , 2012b). The genus Mucilaginibacter comprises 31 species at the time of writing, including some most recently added: Mucilaginibacter polytrichastri (Chen et al , 2014), Mucilaginibacter defluvii (Fu et al , 2014), Mucilaginibacter soyangensis (Joung et al , 2014b), Mucilaginibacter flavus (Joung et al , 2014a), Mucilaginibacter auburnensis (Kämpfer et al , 2014), Mucilaginibacter pineti (Paiva et al , 2014), Mucilaginibacter koreensis (Park et al , 2014).…”

Mucilaginibacter vulcanisilvae sp. nov., isolated from a volcanic forest

“…The genus Mucilaginibacter includes Gram-negative, non-motile rods producing exopolysaccharide, containing straight and branched-chain saturated fatty acids as the major cellular fatty acids, menaquinone 7 (MK-7) as the major isoprenoid quinone and phosphatidylethanolamine as the major polar lipid, and having 39.1–47.2 mol% DNA G+C content (Pankratov et al , 2007; Urai et al , 2008; Baik et al , 2010; Chen et al , 2014). At the time of writing, the genus Mucilaginibacter comprised 32 recognized species (Parte, 2014), which have been isolated from various environmental habitats such as peat bog (Pankratov et al , 2007), tundra soil (Jiang et al , 2012), rice straw (An et al , 2009), rice paddy soil (Jeon et al , 2009), wetland (Baik et al , 2010; Joung et al , 2014a), freshwater (Joung et al , 2014b), rhizosphere (Madhaiyan et al , 2010; Han et al , 2012; Kim et al , 2012a; Lee et al , 2013), decaying lichen (Männistö et al , 2010; Khan et al , 2013a, b), moss (Chen et al , 2014), leaf mould (Park et al , 2014), plant stem (Kämpfer et al , 2014; Paiva et al , 2014), wastewater sewage (Hwang et al , 2014) and marine environments (Kim et al , 2012b; Yoon et al , 2012; Kang et al , 2013).…”