PURPOSE This study was to evaluate the effect of rinsing time on the accuracy of interim crowns fabricated by digital light processing. MATERIALS AND METHODS The maxillary right first molar master die was duplicated using a silicone material, while a study die was produced using epoxy resin. Scans of the epoxy resin die were used in combination with CAD software to design a maxillary right first molar interim crown. Based on this design, 24 interim crowns were fabricated with digital light processing. This study examined the trueness and precision of products that were processed with one of the three different postprocessing rinsing times (1 min, 5 min, and 10 min). Trueness was measured by superimposing reference data with scanned data from external, intaglio, and marginal surfaces. Precision was measured by superimposing the scan data within the group. The trueness and precision data were analyzed using Kruskal-Wallis, nonparametric, and post-hoc tests, and were compared using a Mann-Whitney U test with Bonferroni correction (α=.05). RESULTS The trueness of the external and intaglio surfaces of crowns varied significantly among the different rinsing times ( P =.004, P =.003), but there was no statistically significant difference in terms of trueness measurements of the marginal surfaces ( P =.605). In terms of precision, statistically significant differences were found among the external, intaglio, and marginal surfaces ( P =.001). CONCLUSION Interim crowns rinsed for 10 minutes showed high accuracy.

A novel bacterial strain designated HME7863T was isolated from a freshwater reservoir in

A non-motile, yellow-orange-pigmented bacterial strain, designated HME6664 T , was isolated from Lake Soyang, Republic of Korea. The major fatty acids of strain HME6664 T were summed feature 3 (comprising C 16 : 1 v6c and/or C 16 : 1 v7c; 44.7 %) and iso-C 15 : 0 (20.2 %). The DNA G+C content was 40.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME6664 T formed a lineage within the genus Mucilaginibacter. Strain HME6664 T was closely related to Mucilaginibacter ximonensis (96.7 %), Mucilaginibacter dorajii (96.5 %) and Mucilaginibacter lappiensis (96.3 %). On the basis of the evidence presented in this study, strain HME6664 T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter soyangensis sp. nov., is proposed. The type strain is HME6664 T (5KCTC 23261 T 5CECT 7824 T ).The genus Mucilaginibacter was first proposed by Pankratov et al. (2007) as a member of the family Sphingobacteriaceae. Members of the genus Mucilaginibacter may contain iso-C 15 : 0 as the major fatty acid component along with a number of different hydroxy fatty acids as additional major components. Species of the genus of have been isolated from wide range of terrestrial sites including soil, dried rice straw, freshwater, wetland water, rhizosphere and tidal flat sediment Baik et al., 2010;Jeon et al., 2009;Joung & Joh, 2011;Kang et al., 2011;Kim et al., 2010; Männistö et al., 2010;Madhaiyan et al., 2010;Urai et al., 2008;Yoon et al., 2012). At the time of writing, the genus Mucilaginibacter contains at least 22 species with validly published names, including six recently described species: In the course of a study on the microbial community of Lake Soyang, Republic of Korea (38 u 029 240N 128 u 099 150 E), a yellow-orange-pigmented colony was isolated on R2A agar at 25 u C. The isolate, designated HME6664 T , was incubated on R2A agar at 25 u C and the culture was suspended in water containing 20 % glycerol (w/v) for storage at -80 u C.An almost-complete sequence of the 16S rRNA gene (1330 bp) was obtained for strain HME6664 T . Identification of phylogenetic neighbours and calculation of pairwise 16S rRNA gene sequence similarity were performed by using the EzTaxon-e server (Kim et al., 2012c). Phylogenetic and molecular evolutionary analyses were conducted by using MEGA software version 5 (Tamura et al., 2011). Phylogenetic trees were inferred using the maximum-likelihood (Felsenstein, 1981), maximum-parsimony (Fitch, 1971) and neighbour-joining algorithms (Saitou & Nei, 1987). The robustness of the topologies for the maximum-likelihood and neighbour-joining trees was evaluated by means of bootstrap analysis (Felsenstein, 1985) based on 1000 resamplings of the sequences. All of the phylogenetic trees generated in this study (Fig. 1 The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HME6664 T is HM590829.

A Gram-staining-negative, rod-shaped bacterium, strain HMD2169 T , was isolated from a mesotrophic artificial lake in Korea. Strain HMD2169T grew in the presence of 0-3.0 % (w/v) NaCl, at pH 5-10 and at 20-37 6C. The predominant quinone of strain HMD2169 T was ubiquinone (UQ)-8. The major fatty acids were summed feature 3 (comprising C 16 : 1 v7c and/or C 16 : 1 v6c), C 16 : 0 and summed feature 8 (comprising C 18 : 1 v7c and/or C 18 : 1 v6c). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 59.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD2169 T was a representative of a lineage within the genus Chitinimonas. Strain HMD2169 T was closely related to Chitinimonas taiwanensis (95.8 % sequence similarity) and Chitinimonas koreensis (94.6 %). On the basis of the evidence presented in this study, strain HMD2169

Lacihabitans soyangensis gen. nov., sp. nov., a new member of the family Cytophagaceae, isolated from a freshwater reservoir A Gram-staining-negative, non-motile and orange-pigmented bacterium, designated strain HME6675 T , was isolated from freshwater of a reservoir in Korea. The major fatty acids of strain HME6675 T were iso-C 15 : 0 (33.4 %) and summed feature 3 (comprising C 16 : 1 v6c and/or C 16 : 1 v7c; 31.3 %). The major respiratory quinone was MK-7. The polar lipids were phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and three unidentified polar lipids. The DNA G+C content of strain HME6675 T was 37.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME6675 T formed a lineage within the family Cytophagaceae and was related to Leadbetterella byssophila 4M15 T (93.0 % sequence similarity), Fluviimonas pallidilutea TQQ6 T (90.6 %) and Emticicia oligotrophica GPTSA100-15 T (89.1 %). On the basis of the evidence presented in this study, strain HME6675 T represents a novel genus and species of the family Cytophagaceae, for which the name Lacihabitans soyangensis gen. nov., sp. nov. is proposed. The type strain of Lacihabitans soyangensis is HME6675 T (5KCTC 23259 T 5CECT 7826 T ).