Chitinimonas viridis sp. nov., isolated from a mesotrophic artificial lake

Abstract

A Gram-staining-negative, rod-shaped bacterium, strain HMD2169 T , was isolated from a mesotrophic artificial lake in Korea. Strain HMD2169T grew in the presence of 0-3.0 % (w/v) NaCl, at pH 5-10 and at 20-37 6C. The predominant quinone of strain HMD2169 T was ubiquinone (UQ)-8. The major fatty acids were summed feature 3 (comprising C 16 : 1 v7c and/or C 16 : 1 v6c), C 16 : 0 and summed feature 8 (comprising C 18 : 1 v7c and/or C 18 : 1 v6c). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 59.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD2169 T was a representative of a lineage within the genus Chitinimonas. Strain HMD2169 T was closely related to Chitinimonas taiwanensis (95.8 % sequence similarity) and Chitinimonas koreensis (94.6 %). On the basis of the evidence presented in this study, strain HMD2169

Chitinimonas arctica sp. nov., isolated from Arctic tundra soil

“…The predominant respiratory isoprenoid quinone of strain R3-44 T was ubiquinone-8 (Q-8), which was consistent with other members of the genus Chitinimonas [1][2][3][4][5][6]. The major fatty acids (relative amount >7.0 %) of strain R3-44 T were summed feature 3 (comprising C 16 : 1 ω7c and/or C 16 : 1 ω6c, 40.6 %) and C 16 : 0 (29.3 %).…”

“…The genome contained a wide variety of ABC transporters genes, including those involved in ferric iron, manganese, molybdenum, pyrimidine, urea, vitamin B12 and zinc transport, which may be closely related to the colonization and survival of strain R3-44 T in the polar region environment. Thirty-eight oxidative stress proteins were found in the genome, which may help strain R3-44 T adapt to the extreme [3]. †Data from Padakandla and Chae.…”

“…net/ chitinimonas. html), including Chitinimonas taiwanensis [1], Chitinimonas koreensis [2], Chitinimonas viridis [3], Chitinimonas naiadis [4], Chitinimonas lacunae [5] and Chitinimonas prasina [6]. Members of the genus Chitinimonas are aerobic, rod-shaped, Gram-stain-negative, chitinolytic-active, motile by polar flagellum, negative for indole production and glucose fermentation, and may or may not hydrolyse aesculin, gelatin and urea [1,2].…”

Mycoavidus cysteinexigens gen. nov., sp. nov., an endohyphal bacterium isolated from a soil isolate of the fungus Mortierella elongata

“…The predominant Table 1. Differential characteristics of strain B1-EB T and genera in the family Burkholderiaceae Taxa: 1, B1-EB T ; 2, Burkholderia (Gillis et al, 1995;Yabuuchi et al, 1992); 3, Pandoraea (Anandham et al, 2010;Coenye et al, 2000;Sahin et al, 2011);4, Lautropia (Daneshvar et al, 2001;Gerner-Smidt et al, 1994); 5, Limnobacter (Lu et al, 2011;Spring et al, 2001); 6, Cupriavidus (Vandamme & Coenye, 2004;Vaneechoutte et al, 2004); 7, Ralstonia (Coenye et al, 2003;Yabuuchi et al, 1992); 8, Paucimonas (Jendrossek, 2001); 9, Chitinimonas (Chang et al, 2004;Joung et al, 2014;Kim et al, 2006;Li et al, 2014); 10, Polynucleobacter (Hahn et al, 2009;Hahn et al, 2012). Because of the unexpected affiliation with the family Aquificaceae (from: http://www.bacterio.net/thermothrix.html, 'List of Prokaryotic names with Standing in Nomenclature'), the genus Thermothrix described in the family Burkholderiaceae is excluded from the 49.8 59.0-69.5 61.9-65.8 64.6-65.4 55-59 63-69 63.9-66.6 59¡2 59.8-65.0 40.3-49.4 *Growth at 42 8C.…”

Rubrivirga profundi sp. nov., isolated from deep-sea water, and emended description of the genus Rubrivirga

“…The DNA G+C content was determined by using the thermal denaturation fluorimetic method described by Gonzalez & Saiz-Jimenez (2002) and Joung et al (2014). For the DNA G+C content analysis, a real-time PCR was carried out using the iQ5 thermocycler (Bio-Rad).…”